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Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples.

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Images

Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001), expandable thumbnail
  • Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001), expandable thumbnail
  • Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Suspension cells, Serum, Adherent cells

Assay type

Enzyme activity

Reactive species

Mammals

Assay time

30m

Sensitivity

> 40 mU/mL

Target data

Function

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.

Alternative names

What's included?

100 Test
Components
Assay Buffer II
1 x 25 mL
Developer Solution III
1 x 1 Vial
NADH Standard I
1 x 1 Vial
TPI Enzyme Mix
1 x 1 Vial
TPI Positive Control
1 x 1 Vial
TPI Substrate
1 x 1 Vial

Recommended products

Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Suspension cells, Serum, Adherent cells

Assay type

Enzyme activity

Reactive species

Mammals

Assay time

30m

Assay Platform

Microplate reader

Sensitivity

> 40 mU/mL

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.

The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.

TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min

This product is manufactured by BioVision, an Abcam company and was previously called K670 Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit. K670-100 is the same size as the 100 test size of ab197001.

Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.

In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Triosephosphate isomerase also known as TPI is an enzyme that plays an important role in glycolysis and gluconeogenesis by catalyzing the reversible isomerization of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate (G3P). It has a molecular mass of approximately 27 kDa. This enzyme is widely expressed in many tissues with high presence in red blood cells muscle and brain tissues due to their high energy demands. As a dimer enzyme it exhibits significant catalytic efficiency that supports cellular energy management.

Biological function summary

The enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.

Pathways

Triosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.

Associated diseases and disorders

Mutations or deficiencies in triosephosphate isomerase can lead to conditions such as triosephosphate isomerase deficiency a rare autosomal recessive disorder characterized by progressive neurological dysfunction hemolytic anemia and muscle weakness. This deficiency arises from dysfunctional or unstable isomerase activity leading to an accumulation of DHAP. Additionally research has shown links between this enzyme and Alzheimer's disease where altered glucose metabolism is a common feature. Abnormal triosephosphate isomerase activity may interact with amyloid precursor protein processing in the disease pathology.

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3 product images

  • Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001), expandable thumbnail

    Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001)

    Relative TPI activity was calculated in lysates prepared from rat muscle (0.5 μg), MCF-7 cells (0.8 μg), and HeLa cells (0.84 μg).

  • Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001), expandable thumbnail

    Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001)

    Typical NADH Standard Curve obtained following assay protocol.

  • Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001), expandable thumbnail

    Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001)

    Kinetic measurement of Triose Phosphate Isomerase (TPI) activity in various samples.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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