Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric)
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(3 Publications)
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples.
View Alternative Names
TPI, TPI1, Triosephosphate isomerase, TIM, Methylglyoxal synthase, Triose-phosphate isomerase
- FuncS
Supplier Data
Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001)
Relative TPI activity was calculated in lysates prepared from rat muscle (0.5 μg), MCF-7 cells (0.8 μg), and HeLa cells (0.84 μg).
- FuncS
Supplier Data
Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001)
Typical NADH Standard Curve obtained following assay protocol.
- FuncS
Supplier Data
Functional Studies - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001)
Kinetic measurement of Triose Phosphate Isomerase (TPI) activity in various samples.
- Schematic Diagram
Supplier Data
Schematic Diagram - Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (AB197001)
Representative image of Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) ab197001
Components shown from left to right :
- NADH Standard I
- TPI Substrate
- Developer Solution III
- TPI Enzyme Mix
- TPI Positive Control
- Assay Buffer 2
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Reactivity data
Product details
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.
TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min
This product is manufactured by BioVision, an Abcam company and was previously called K670 Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit. K670-100 is the same size as the 100 test size of ab197001.
Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.
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Biological function summary
The enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.
Pathways
Triosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.
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Publications (3)
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ACS chemical biology 17:2769-2780 PubMed35951581
2022
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Frontiers in endocrinology 13:896558 PubMed35903269
2022
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Acta neuropathologica communications 8:65 PubMed32381112
2020
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