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AB102531

Trypsin Activity Assay Kit (Colorimetric)

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(26 Publications)

Trypsin Activity Assay Kit (Colorimetric) ab102531 is a simple and fast assay to quantify trypsin activity. Readout on any colorimetric (405 nm) plate reader.

- Kit includes standard curve for quantitation, inhibitor controls, and positive control enzyme

View Alternative Names

Trypsin

5 Images
Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)
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Lab

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

p-NA standard curve measured in duplicates ((+/- SD), background subtracted) after 60 minutes incubation.

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)
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Lab

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

Trypsin levels were kinetically measured in commercially purchased trypsin (1x); background signal subtracted (duplicates +/- SD).

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)
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Supplier Data

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

Example of Trypsin sample timeline obtained using ab102531

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)
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Supplier Data

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

Example of standard curve obtained using ab102531, following assay protocol. The curve should be used as guideline only.

Schematic Diagram - Trypsin Activity Assay Kit (Colorimetric) (AB102531)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

Representative image of Trypsin Activity Assay Kit (Colorimetric) ab102531

Components shown from left to right :

- pNA Standard II

- Trypsin Substrate

- Trypsin Positive Control

- Trypsin Inhibitor

- Chymotrypsin Inhibitor II

- Assay Buffer 1

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Sensitivity

= 10 µU

Assay time

2h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

How the assay works

In the trypsin activity assay protocol, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.

Trypsin Activity Assay Kit (Colorimetric) protocol summary :

- Add samples, positive control and standards to wells
- Add chymotrypsin inhibitor to sample and positive control wells and incubate for 10 min at room temp
- Add reaction mix and incubate 25°C protected from light
- Measure optical density (OD 405nm) in a kinetic mode at 25°C for 1 – 2 hours protected from light

How other researchers are using

Trypsin Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse intestinal tissues 1
Mouse Cecal tissue 2
Mouse Pancreatic Acinar Cells 3
References:

1-Bianchimano P et al. 2023
2-Bolsega S et al. 2023
3-Yang L et al. 2023

Related and recommended products

For fluorometric detection, we recommend Trypsin Activity Assay Kit (Fluorometric) ab282916

This product is manufactured by BioVision, an Abcam company and was previously called K771 Trypsin Activity Colorimetric Assay Kit. K771-100 is the same size as the 100 test size of ab102531.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab102531-2000Test", "assetComponentDetails": [ { "size":"20 x 200 µL", "name":"Trypsin Substrate", "number":"AB102531-CMP06" }, { "size":"20 x 1 Vial", "name":"Trypsin Positive Control", "number":"AB102531-CMP03" }, { "size":"20 x 100 µL", "name":"Trypsin Inhibitor", "number":"AB102531-CMP05" }, { "size":"20 x 100 µL", "name":"Chymotrypsin Inhibitor II", "number":"AB102531-CMP02" }, { "size":"20 x 25 mL", "name":"Assay Buffer 1", "number":"AB102531-CMP04" }, { "size":"20 x 400 µL", "name":"pNA Standard II", "number":"AB102531-CMP01" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102531-100Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Trypsin Substrate", "number":"AB102531-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Trypsin Positive Control", "number":"AB102531-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Trypsin Inhibitor", "number":"AB102531-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Chymotrypsin Inhibitor II", "number":"AB102531-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 1", "number":"AB102531-CMP04", "productcode":"" }, { "size":"1 x 400 µL", "name":"pNA Standard II", "number":"AB102531-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.
Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.
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Product protocols

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Target data

See full target information TRYP_PIG
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1499339 PubMed40510743

2025

Embryo-derived trypsin-induced calcium entry is inhibited by endometrial infertility factor, LEFTY2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqi Yang,Jing Yan,Steffen Kull,Md Alauddin,Sara Y Brucker,Melanie Henes,Florian Lang,Madhuri S Salker

Journal of microbiology and biotechnology 35:e2412035 PubMed40081888

2025

HP7 Improves Gastric Emptying by Modulating Digestive Factors in a Loperamide-Induced Functional Dyspepsia Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Woong Jeong,Daehyeop Lee,Hyeonji Kim,Hyeonjun Gwon,Kippeum Lee,Joo-Yun Kim,Jae-Jung Shim,Jae-Hwan Lee

Aquaculture nutrition 2025:7291857 PubMed40066367

2025

Expanding the Ingredient Basket in Aquaculture: Growth Performance and Feed Utilization of Australian Hybrid Abalone () Fed Methanotrophic Single Cell Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Salini,Weifang Wang,Thomas S Mock,Muhammad A B Siddik,Matthew K Jago,Kelsey L F Bews,David S Francis

Journal of proteome research 23:3791-3805 PubMed38980715

2024

The Spatial Extracellular Proteomic Tumor Microenvironment Distinguishes Molecular Subtypes of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jade K Macdonald,Harrison B Taylor,Mengjun Wang,Andrew Delacourt,Christin Edge,David N Lewin,Naoto Kubota,Naoto Fujiwara,Fahmida Rasha,Cesia A Marquez,Atsushi Ono,Shiro Oka,Kazuaki Chayama,Sara Lewis,Bachir Taouli,Myron Schwartz,M Isabel Fiel,Richard R Drake,Yujin Hoshida,Anand S Mehta,Peggi M Angel

Animals : an open access journal from MDPI 14: PubMed38929368

2024

Effect of a Multi-Species Direct-Fed Microbial on Growth Performance, Nutrient Digestibility, Intestinal Morphology and Colonic Volatile Fatty Acids in Weanling Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Panumas Kongpanna,John A Doerr,Dachrit Nilubol,Uttra Jamikorn

Biomolecules 14: PubMed38927046

2024

Redefining Immune Dynamics in Acute Pancreatitis: The Protective Role of Galectin-3 Deletion and Treg Cell Enhancement.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Milivojcevic Bevc,Danijela Tasic-Uros,Bojana S Stojanovic,Ivan Jovanovic,Milica Dimitrijevic Stojanovic,Nevena Gajovic,Milena Jurisevic,Gordana Radosavljevic,Jelena Pantic,Bojan Stojanovic

Molecular & cellular proteomics : MCP 23:100745 PubMed38447790

2024

Universal Pretreatment Development for Low-input Proteomics Using Lauryl Maltose Neopentyl Glycol.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Konno,Masaki Ishikawa,Daisuke Nakajima,Yusuke Endo,Osamu Ohara,Yusuke Kawashima

Journal of the American Society for Mass Spectrometry 35:487-497 PubMed38329320

2024

Differential Protease Specificity by Collagenase as a Novel Approach to Serum Proteomics That Includes Identification of Extracellular Matrix Proteins without Enrichment.

Applications

Unspecified application

Species

Unspecified reactive species

Jade K Macdonald,Cassandra L Clift,Janet Saunders,Stephen C Zambrzycki,Anand S Mehta,Richard R Drake,Peggi M Angel

Aquaculture nutrition 2023:8887768 PubMed37885797

2023

Sustainable Aquafeed Formulations Containing Insect Larval Meal and Grape Marc for the New Zealand Farmed Abalone.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Bullon,Andrea C Alfaro,Moganakumaar Manivannan,Seyedehsara Masoomi Dezfooli,Ali Seyfoddin

iScience 26:108143 PubMed37915599

2023

Oral vancomycin treatment suppresses gut trypsin activity and preserves intestinal barrier function during EAE.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Bianchimano,Kacper Iwanowski,Emma M Smith,Adam Cantor,Paola Leone,Gerold Bongers,Carlos G Gonzalez,Yoon Hongsup,Joshua Elias,Howard L Weiner,Jose C Clemente,Stephanie K Tankou
View all publications
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