Trypsin Activity Assay Kit (Colorimetric)

Trypsin Activity Assay Kit (Colorimetric) ab102531 is simple and fast assay to quantify trypsin activity.

In the trypsin activity assay protocol, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.

Trypsin activity assay protocol summary:
- add samples, positive control and standards to wells
- add chymotrypsin inhibitor to sample and positive control wells and incubate for 10 min at room temp
- add reaction mix
- analyze every 2-3 min for 1-2 hr with microplate reader in kinetic mode at 25°C

This product is manufactured by BioVision, an Abcam company and was previously called K771 Trypsin Activity Colorimetric Assay Kit. K771-100 is the same size as the 100 test size of ab102531.