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TUNEL Assay Kit - BrdU-Red ab66110 uses a convenient and sensitive method to detect DNA fragmentation by flow cytometry and fluorescence microscopy in live cells.

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Reactive species

Mammals

Assay time

3h

Images

What's included?

60 Test
Components
7-AAD/RNase Staining Buffer
1 x 30 mL
Anti-BrdU-Red Antibody
1 x 300 µL
Br-dUTP
1 x 480 µL
Negative Control Cells
1 x 5 mL
Positive Control Cells
1 x 5 mL
Reaction Buffer
1 x 600 µL
Rinse Buffer
1 x 120 mL
TdT Enzymes
1 x 45 µL
Wash Buffer
1 x 120 mL

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TUNEL Assay Kit - BrdU-Red ab66110 uses a convenient and sensitive method to detect DNA fragmentation by flow cytometry and fluorescence microscopy in live cells.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Reactive species

Mammals

Assay time

3h

Assay Platform

Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

TUNEL Assay Kit - BrdU-Red ab66110 uses a convenient and sensitive method to detect DNA fragmentation by flow cytometry and fluorescence microscopy in live cells.

The TUNEL assay is used to detect DNA fragmentation, such as in apoptosis. It uses terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of deoxynucleotides at the free 3'-hydroxyl ends of fragmented DNA. The deoxynucleotides are then labeled in a variety of ways for detection of the degree of DNA fragmentation.

This TUNEL assay protocol is based on Br-dUTP (bromolated deoxyuridine triphosphate nucleotide), which can be more readily incorporated into DNA strand breaks by the TdT enzyme than other dUTP labels such as FITC, biotin or dioxigenin. The greater incorporation rate produces a brighter signal when the Br-dUTP sites are detected with an anti-BrdU monoclonal antibody directly labeled with a red fluorochrome.

The BrdU-Red signal can be analyzed at Ex/Em 488/576 nm, with an optional 7-AAD counterstain at Ex/Em 488/655nm.

This TUNEL assay kit includes both negative and positive control cells. It is designed to be suitable for studying DNA fragmentation in GFP-transfected cells.

Tunel assay protocol summary:
- fix cells / tissues with formaldehyde, or deparaffinize and rehydrate if paraffin sections, and wash
- incubate cells in 70% ethanol for 30 min at 4°C, or if tissues incubate with proteinase K solution for 5 min at room temp and refix with formaldehyde
- wash
- incubate in DNA labeling solution for 60 min at 37°C
- wash
- incubate in antibody solution for 30 min at room temp
- add 7-AAD / RNase A solution and incubate for 30 min at room temp
- analyze with flow cytometry or fluorescent microscopy

This kit is BrdU-Red labeled (Ex/Em = 488/576 nm). It was previously called TUNEL Assay Kit - In situ BrdU-Red DNA Fragmentation.To use FITC (Ex/Em = 495/519 nm) as a label, we recommend .For chromogenic TUNEL staining, we recommend .Find out more about the TUNEL method in the .

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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