TUNEL Assay Kit - FITC

Product details

How the assay works

TUNEL assay - FITC ab66108 is used to detect DNA fragmentation in apoptosis.

The TUNEL assay - FITC used to detect DNA fragmentation, such as in apoptosis. It uses terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of deoxynucleotides at the free 3'-hydroxyl ends of fragmented DNA. The deoxynucleotides are then labeled in a variety of ways for detection of the degree of DNA fragmentation.

The TUNEL assay protocol in this kit uses the deoxynucleotide fluorescein-12-dUTP. DNA which has been labeled with fluorescein can then be analyzed by flow cytometry or fluoresence microscopy with Ex/Em 488/520 nm. Propidium iodide is also included in this kit as a counterstain with Ex/Em 488/623 nm.

TUNEL assay protocol summary:

- fix cells with formaldehyde for 15 min on ice
- wash with PBS
- add ice-cold 70% ethanol and incubate for 30 min
- pellet cells and resuspend in wash buffer and wash again
- pellet cells and resuspend in staining solution and incubate for 60 min at 37°C
- add rinse buffer, pellet cells and discard supernatant, and rinse again
- resuspend cells in propidium iodide/RNAse A solution and incubate for 30 min at room temp
- analyze with flow cytometer or fluoresence microscope

To use HRP detection, we recommend TUNEL Assay Kit - HRP-DAB ab206386 . To use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend ab66110.

How other researchers are using

TUNEL Assay Kit - FITC has been used in variety of sample type including:
- Human lung cancer cell lines A549 and Human colon cancer cell lines HCT-116 1
- Mouse lung tissue
- Mouse Mesenchymal stem cell (MSC) 3

References:
1 - Olszewski M et al. 2024; 2 - Zhang P et at 2024; 3 - Zhang T et al. 2023