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TUNEL Assay Kit - HRP-DAB ab206386 allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, frozen tissue sections, or in preparations of single cell suspensions fixed on slides.

Detection method

Colorimetric

Sample types

Tissue, Adherent cells

Assay type

Cell-based (qualitative)

Assay time

5h

Images

What's included?

60 Slide
Components
25X Conjugate
1 x 300 µL
Blocking Buffer
1 x 24 mL
DAB Solution 1 (DAB Concentrate)
1 x 300 µL
DAB Solution 2 (Substrate Reaction Buffer)
1 x 8 mL
Methyl Green Counterstain
2 x 3.5 mL
Proteinase K
1 x 100 µL
Stop Buffer
1 x 8 mL
TdT Enzyme
1 x 70 µL
TdT Equilibration Buffer
1 x 8 mL
TdT Labeling Reaction Mix
2 x 1.3 mL

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TUNEL Assay Kit - HRP-DAB ab206386 allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, frozen tissue sections, or in preparations of single cell suspensions fixed on slides.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Adherent cells

Assay type

Cell-based (qualitative)

Assay time

5h

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

TUNEL Assay Kit - HRP-DAB ab206386 allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, frozen tissue sections, or in preparations of single cell suspensions fixed on slides.

The TUNEL assay is used to detect DNA fragmentation, such as in apoptosis. It uses terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of deoxynucleotides at the free 3'-hydroxyl ends of fragmented DNA. The deoxynucleotides are then labeled in a variety of ways for detection of the degree of DNA fragmentation.

In this TUNEL assay protocol:
- terminal deoxynucleotidyl Transferase (TdT) binds to exposed 3'-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of biotin-labeled deoxynucleotides
- biotinylated nucleotides are bound with a streptavidin-horseradish peroxidase (HRP) conjugate
- diaminobenzidine (DAB) reacts with the HRP labeled sample to generate an insoluble colored (brown) substrate at the site of DNA fragmentation
- counterstaining with methyl green aids in the evaluation of normal and apoptotic cells

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This kit is designed for chromogenic TUNEL staining with HRP and DAB. It was previously called In situ Apoptosis Detection Kit (DAB). To use FITC (Ex/Em = 495/519 nm) as a label, we recommend . To use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend . Find out more about the TUNEL method in the . **The methyl green counterstain is water soluble and so a non-aqueous/organic mounting media needs to be used.** **How other researchers have used HRP-DAB TUNEL Assay Kit ab206386** This TUNEL assay kit has been used in publications in a variety of sample types, including: - Human: LX-2 cell cultures1 - Mouse (paraffin-embedded sections): abdominal aortic aneurysm lesion2, thyroid gland3, lung tissue4, heart5, liver6, ovary tissue7, skin8, liver9 - Rat: paraffin embedded heart10, brain tissue11 - Other: paraffin embedded human xenograft tumors in mice12, paraffin-embedded tissue from nude mice injected with human pancreatic cancer cell line13 References: 1-Park SM et al 2017, 2-Li et al 2019, 3-Iglesias-Osma MC et al 2019, 4-Yuan X et al 2018, 5-Ravi V et al 2018, 6-Jadhav K et al 2018, 7-Gao Y et al 2017, 8-Shin JM et al 2017, 9-Kim MH et al 2017 and 2016, 10-Zhang J et al 2018, 11-Xiao B et al 2017, 12-Nielsen CF et al 2017, 13-Dauer et al 2019 Shipped at 2-8°C.

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