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AB206386

TUNEL Assay Kit - HRP-DAB

3

(2 Reviews)

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(213 Publications)

TUNEL Assay Kit - HRP-DAB ab206386 allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, frozen tissue sections, or in preparations of single cell suspensions fixed on slides. The TUNEL assay is widely used to detect and quantify DNA fragmentation and apoptosis.

- Cited in over 200 publications
3 Images
Immunohistochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)
  • IHC

PubMed

Immunohistochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)

TUNEL assay staining.

Gao Y et al used In situ Apoptosis Detection Kit / TUNEL assay ab206386 to analyze tissue sections from mouse ovaries.

a. Section treated with DNase I as positive control

b. Negative control without TdT enzyme

c and f. representative experimental images.

Nuclei stained with the TUNEL assay are brown. Sections were counter-stained with Methyl Green.

Gao Y et al. Reproductive Biology and Endocrinology 15:94 (2017) Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Immunocytochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)
  • ICC

Supplier Data

Immunocytochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)

Using paraffin fixed human tonsil tissue, 10 μm sections (1000X)

Immunohistochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)
  • IHC

Supplier Data

Immunohistochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)

Using paraffin fixed human tonsil tissue,10 μm sections (1000X). A] Section processed and counter-stained with methyl green according to the manual. B] Counter-stain step was eliminated to more clearly illustrate the level of positive staining in the germinal centres of tonsil tissue. C] Section treated with DNase I in order to generate a positive control slide. Note all nuclei stain positive. The use of DNase I generates free 3'-OH groups on cellular DNA, these free 3'-OH groups are then labelled with biotin-nucleotide by the TdT in the kit. D] Negative control, the TdT enzyme step was eliminated thereby generating a negative slide.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Adherent cells

Assay type

Cell-based (qualitative)

Assay time

5h

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Product details

TUNEL Assay Kit ab206386 is used to detect DNA fragmentation, such as in apoptosis. TUNEL assay kits are sometimes also called In Situ Cell Death Detection Kits.

How the TUNEL assay works

The TUNEL assay exploits the fact that apoptotic endonucleases not only affect cellular DNA by producing the classical DNA ladder, but also generate free 3’-OH groups at the ends of these DNA fragments. These free 3’-OH groups are end-labeled in the TUNEL assay allowing for the detection of apoptotic cells using a molecular biology-based, end labeling technique.

The TUNEL assays uses terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of deoxynucleotides at the free 3'-hydroxyl ends of fragmented DNA. The deoxynucleotides are then labeled in a variety of ways for detection of the degree of DNA fragmentation.

TUNEL assay protocol summary
In this TUNEL assay protocol:

  • - Terminal deoxynucleotidyl Transferase (TdT) binds to exposed 3'-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of biotin-labeled deoxynucleotides.
  • - Biotinylated nucleotides are bound with a streptavidin-horseradish peroxidase (HRP) conjugate.
  • - Diaminobenzidine (DAB) reacts with the HRP labeled sample to generate an insoluble colored (brown) substrate at the site of DNA fragmentation.
  • - Counterstaining with methyl green aids in the evaluation of normal and apoptotic cells.

Related TUNEL assay products
This kit is designed for chromogenic TUNEL staining with HRP and DAB. To use FITC (Ex/Em = 495/519 nm) as a label, we recommend ab66108 . To use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend ab66110.

How other researchers are using TUNEL assay kit ab206386
This TUNEL assay kit has been cited in several publications in a variety of sample types, including:

  • - Human: Precision-cut intestinal slices (hPCIS)1 (paraffin-embedded sections), human gastric cancer cell culture2 (single cell suspension)
  • - Mouse: abdominal aortic aneurysm lesion3, thyroid gland4, lung5, heart6, pancreas7, ovary8, skin9, liver10 (paraffin-embedded sections), brain11 (frozen sections)
  • - Rat: heart12, brain13 (paraffin-embedded sections)
  • - Other: human xenograft tumours in mice14, tissue from nude mice injected with human pancreatic cancer cell line 15,  pig heart16 (paraffin-embedded sections)

References: 1- PMID: 35428896; 2- PMID: 34440988; 3- PMID: 30651000; 4-  PMID: 30952933; 5- PMID: 40084179;  6- PMID: 31886717; 7- PMID: 36458554; 8- PMID: 29379893, PMID: 39469582; 9- PMID: 33690222; 10- PMID: 36911196; PMID: 35970323; PMID: 35763355; 11- PMID: 34518549; 12- PMID: 34543287; PMID: 32536996; 13- PMID: 38413663; 14- PMID: 35265825; 15- PMID: 30755605; 16- PMID: 32784904

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (213)

Recent publications for all applications. Explore the full list and refine your search

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Se-Weon Park,Joseph H C Lai,Xiongqi Han,Vivian W M Leung,Peng Xiao,Jianpan Huang,Kannie W Y Chan

Nature communications 15:137 PubMed38167344

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p53 suppresses MHC class II presentation by intestinal epithelium to protect against radiation-induced gastrointestinal syndrome.

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Jianming Wang,Chun-Yuan Chang,Xue Yang,Fan Zhou,Juan Liu,Jill Bargonetti,Lanjing Zhang,Ping Xie,Zhaohui Feng,Wenwei Hu

Nature communications 14:8474 PubMed38123539

2023

Mtfp1 ablation enhances mitochondrial respiration and protects against hepatic steatosis.

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Cecilia Patitucci,Juan Diego Hernández-Camacho,Elodie Vimont,Sonny Yde,Thomas Cokelaer,Thibault Chaze,Quentin Giai Gianetto,Mariette Matondo,Anastasia Gazi,Ivan Nemazanyy,David A Stroud,Daniella H Hock,Erminia Donnarumma,Timothy Wai

Cell death & disease 14:848 PubMed38123563

2023

Atypical cell death and insufficient matrix organization in long-bone growth plates from Tric-b-knockout mice.

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Atsuhiko Ichimura,Yuu Miyazaki,Hiroki Nagatomo,Takaaki Kawabe,Nobuhisa Nakajima,Ga Eun Kim,Masato Tomizawa,Naoki Okamoto,Shinji Komazaki,Sho Kakizawa,Miyuki Nishi,Hiroshi Takeshima

Redox biology 69:102984 PubMed38061207

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Dimethyl malonate preserves renal and mitochondrial functions following ischemia-reperfusion via inhibition of succinate dehydrogenase.

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Mattias Carlström,Lucas Rannier Ribeiro Antonino Carvalho,Drielle Guimaraes,Ariela Boeder,Tomas A Schiffer

Molecular neurobiology 61:3397-3408 PubMed37989982

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MiR-184 Mediated the Expression of ZNF865 in Exosome to Promote Procession in the PD Model.

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Chang Liu,Yang Wang,Jing-Wen Li,Xiaoyan Zhu,Hai-Song Jiang,Hong Zhao,Li-Ming Zhang

Development (Cambridge, England) 150: PubMed37800308

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Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis.

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Gina E Merges,Lena Arévalo,Andjela Kovacevic,Keerthika Lohanadan,Dirk G de Rooij,Carla Simon,Melanie Jokwitz,Walter Witke,Hubert Schorle

Frontiers in immunology 14:1254762 PubMed37908354

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Schistosomiasis-associated pulmonary hypertension unveils disrupted murine gut-lung microbiome and reduced endoprotective Caveolin-1/BMPR2 expression.

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Ygor Marinho,Elizabeth S Villarreal,Sammy Y Aboagye,David L Williams,Jun Sun,Claudia L M Silva,Sarah E Lutz,Suellen D Oliveira

Experimental & molecular medicine 55:2220-2237 PubMed37779142

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Oncogenic KRAS mutation confers chemoresistance by upregulating SIRT1 in non-small cell lung cancer.

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Dong Hoon Shin,Jeong Yeon Jo,Minyoung Choi,Kyung-Hee Kim,Young-Ki Bae,Sang Soo Kim

Scientific reports 13:15544 PubMed37731032

2023

Galantamine improves glycemic control and diabetic nephropathy in Lepr mice.

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Species

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Qinghe Meng,Julia Ma,Liye Suo,Napat Pruekprasert,Prithi Chakrapani,Robert N Cooney
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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