Tyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples.
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- Molecules (Basel, Switzerland) 27:2022The Combination of Niacinamide, Vitamin C, and PDRN Mitigates Melanogenesis by Modulating Nicotinamide Nucleotide Transhydrogenase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Jun Park,Kyung-A Byun,Seyeon Oh,Hyoung Moon Kim,Moon Suk Chung,Kuk Hui Son,Kyunghee ByunPubMed 35956878
- Oncology letters 23:1692022Tyrosinase suppresses vasculogenic mimicry in human melanoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Kamo,Ryota Kawahara,Siro SimizuPubMed 35496574
- Theranostics 12:2063-20792022Heterogeneity of tyrosine-based melanin anabolism regulates pulmonary and cerebral organotropic colonization microenvironment of melanoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuefeng Wang,Yu Chen,Bin Lan,Yu Wang,Wansong Lin,Xu Jiang,Jiayin Ye,Bingxue Shang,Chao Feng,Jun Liu,Jingjie Zhai,Muhan Xu,Qing Li,Liangyu Lin,Mingyuan Hu,Fanjun Zheng,Ling Chen,Changshun Shao,Ying Wang,Yufang ShiPubMed 35265199
- Molecules (Basel, Switzerland) 27:2022Radiofrequency Irradiation Mitigated UV-B-Induced Skin Pigmentation by Increasing Lymphangiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyoung Moon Kim,Seyeon Oh,Kyung-A Byun,Jin Young Yang,Hye Jin Sun,Donghwan Kang,Kuk Hui Son,Kyunghee ByunPubMed 35056769
Key facts
- Detection method
Colorimetric
- Sample types
Purified protein, Tissue Lysate, Tissue Homogenate, Cell Lysate
- Assay type
Enzyme activity (quantitative)
Target data
Function
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (By similarity). Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (By similarity).
Alternative names
Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
What's included?
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Tyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples.
Alternative names
Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
Key facts
- Detection method
Colorimetric
- Sample types
Purified protein, Tissue Lysate, Tissue Homogenate, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Tyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K742 Tyrosinase Activity Assay Kit (Colorimetric). K742-100 is the same size as the 100 test size of ab252899.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosinase also known as monophenol monooxygenase is a copper-containing enzyme with a mass of approximately 60-70 kDa. It catalyzes the oxidation of phenols such as tyrosine to quinones which are important precursors for melanin synthesis. Tyrosinase is predominantly expressed in melanocytes the cells responsible for pigment production in skin hair and eyes. Its expression also occurs in other tissues like the brain where it might play different roles.
- Biological function summary
Within melanin production tyrosinase functions as the rate-limiting enzyme initiating the synthesis path by converting tyrosine into dopaquinone. This enzyme works as part of the melanogenic complex interacting with other enzymes such as tyrosinase-related proteins 1 and 2 to regulate melanin synthesis. The presence and activity of tyrosinase significantly impacts pigmentation processes and skin coloration.
- Pathways
Relating tyrosinase to melanin biosynthetic and regulatory pathways reveal its central role. The enzyme is vital in the Raper-Mason pathway for melanin production where it interacts with proteins such as dopachrome tautomerase. It bridges the gap between external stimuli like UV radiation and cellular responses transforming them into pigmentation changes. The L-tyrosine and catecholamine biosynthesis pathways also influence tyrosinase activity linking it to broader metabolic processes.
- Associated diseases and disorders
Issues with tyrosinase can lead to conditions like albinism and melanoma. In oculocutaneous albinism type 1 (OCA1) a genetic mutation in the tyrosinase gene results in reduced or absent enzymatic activity causing hypopigmentation. In melanoma abnormal regulation of tyrosinase and its pathway associates with tumor progression and metastasis often in connection with factors such as microphthalmia-associated transcription factor (MITF) that modulate its expression.
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4 product images
Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (ab252899)
Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (ab252899)
Enzyme kinetics for tyrosinase positive control and for potato lysate (16 μg protein).
Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (ab252899)
Enzyme kinetics for tyrosinase activity in uninduced melanoma cells cultured in EMEM + 10% FBS (15 μg protein), and melanoma cells induced for increased tyrosinase activity by culturing for 4 days in EMEM + 0.5% FBS, supplemented with 500 μM cAMP, 100 μM PDE inhibitor IBMX and 100 μM Cu2+ (10 μg protein).
Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (ab252899)
Tyrosinase specific activity in potato lysate and melanoma cells.
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