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AB252899

Tyrosinase Activity Assay Kit (Colorimetric)

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(5 Publications)

Tyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a mix-and-read, one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. Readout on any (510 nm) colorimetric plate reader.

View Alternative Names

Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR

4 Images
Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)
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Supplier Data

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)

Enzyme kinetics for tyrosinase activity in uninduced melanoma cells cultured in EMEM + 10% FBS (15 μg protein), and melanoma cells induced for increased tyrosinase activity by culturing for 4 days in EMEM + 0.5% FBS, supplemented with 500 μM cAMP, 100 μM PDE inhibitor IBMX and 100 μM Cu2+ (10 μg protein).

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)
  • FuncS

Supplier Data

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)

Enzyme kinetics for tyrosinase positive control and for potato lysate (16 μg protein).

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)
  • FuncS

Supplier Data

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)

Chromophore Standard curve

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)
  • FuncS

Supplier Data

Functional Studies - Tyrosinase Activity Assay Kit (Colorimetric) (AB252899)

Tyrosinase specific activity in potato lysate and melanoma cells.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Tissue Homogenate, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Tyrosinase Activity Assay Kit (Colorimetric) (ab252899) is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.

Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K742 Tyrosinase Activity Assay Kit (Colorimetric). K742-100 is the same size as the 100 test size of ab252899.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab252899-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Tyrosinase Substrate", "number":"AB252899-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Tyrosinase", "number":"AB252899-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Tyrosinase Assay Buffer", "number":"AB252899-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"Chromophore Standard", "number":"AB252899-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Tyrosinase Probe", "number":"AB252899-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosinase also known as monophenol monooxygenase is a copper-containing enzyme with a mass of approximately 60-70 kDa. It catalyzes the oxidation of phenols such as tyrosine to quinones which are important precursors for melanin synthesis. Tyrosinase is predominantly expressed in melanocytes the cells responsible for pigment production in skin hair and eyes. Its expression also occurs in other tissues like the brain where it might play different roles.
Biological function summary

Within melanin production tyrosinase functions as the rate-limiting enzyme initiating the synthesis path by converting tyrosine into dopaquinone. This enzyme works as part of the melanogenic complex interacting with other enzymes such as tyrosinase-related proteins 1 and 2 to regulate melanin synthesis. The presence and activity of tyrosinase significantly impacts pigmentation processes and skin coloration.

Pathways

Relating tyrosinase to melanin biosynthetic and regulatory pathways reveal its central role. The enzyme is vital in the Raper-Mason pathway for melanin production where it interacts with proteins such as dopachrome tautomerase. It bridges the gap between external stimuli like UV radiation and cellular responses transforming them into pigmentation changes. The L-tyrosine and catecholamine biosynthesis pathways also influence tyrosinase activity linking it to broader metabolic processes.

Issues with tyrosinase can lead to conditions like albinism and melanoma. In oculocutaneous albinism type 1 (OCA1) a genetic mutation in the tyrosinase gene results in reduced or absent enzymatic activity causing hypopigmentation. In melanoma abnormal regulation of tyrosinase and its pathway associates with tumor progression and metastasis often in connection with factors such as microphthalmia-associated transcription factor (MITF) that modulate its expression.
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Product protocols

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Target data

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed : 28661582).
See full target information Tyrosinase
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 22: PubMed39330302

2024

Fermented Fish Collagen Attenuates Melanogenesis via Decreasing UV-Induced Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung-A Byun,So Young Lee,Seyeon Oh,Sosorburam Batsukh,Jong-Won Jang,Bae-Jin Lee,Kyoung-Min Rheu,Sichao Li,Min-Seok Jeong,Kuk Hui Son,Kyunghee Byun

Molecules (Basel, Switzerland) 27: PubMed35956878

2022

The Combination of Niacinamide, Vitamin C, and PDRN Mitigates Melanogenesis by Modulating Nicotinamide Nucleotide Transhydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Jun Park,Kyung-A Byun,Seyeon Oh,Hyoung Moon Kim,Moon Suk Chung,Kuk Hui Son,Kyunghee Byun

Oncology letters 23:169 PubMed35496574

2022

Tyrosinase suppresses vasculogenic mimicry in human melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Kamo,Ryota Kawahara,Siro Simizu

Theranostics 12:2063-2079 PubMed35265199

2022

Heterogeneity of tyrosine-based melanin anabolism regulates pulmonary and cerebral organotropic colonization microenvironment of melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Wang,Yu Chen,Bin Lan,Yu Wang,Wansong Lin,Xu Jiang,Jiayin Ye,Bingxue Shang,Chao Feng,Jun Liu,Jingjie Zhai,Muhan Xu,Qing Li,Liangyu Lin,Mingyuan Hu,Fanjun Zheng,Ling Chen,Changshun Shao,Ying Wang,Yufang Shi

Molecules (Basel, Switzerland) 27: PubMed35056769

2022

Radiofrequency Irradiation Mitigated UV-B-Induced Skin Pigmentation by Increasing Lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyoung Moon Kim,Seyeon Oh,Kyung-A Byun,Jin Young Yang,Hye Jin Sun,Donghwan Kang,Kuk Hui Son,Kyunghee Byun
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