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AB204715

Tyrosinase Inhibitor Assay Kit (Colorimetric)

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(10 Publications)

Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test to screen tyrosinase inhibitors.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR

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Inhibition Assay - Tyrosinase Inhibitor Assay Kit (Colorimetric) (AB204715)
  • Inhib

Supplier Data

Inhibition Assay - Tyrosinase Inhibitor Assay Kit (Colorimetric) (AB204715)

Inhibition of Tyrosinase Enzymatic Activity with Kojic Acid.

Other - Tyrosinase Inhibitor Assay Kit (Colorimetric) (AB204715)
  • Other

Supplier Data

Other - Tyrosinase Inhibitor Assay Kit (Colorimetric) (AB204715)

Representative image of Tyrosinase Inhibitor Assay Kit (Colorimetric) ab204715

Components shown from left to right :

- Tyrosinase Enhancer

- Tyrosinase

- Tyrosine

- Kojic Acid

- Assay Buffer 42

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate reader

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Product details

Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test to screen tyrosinase inhibitors.

Tyrosinase assay principle
In the tyrosinase assay, tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of kojic acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The absorbance values are used to calculate the tyrosinase activity of the samples

Tyrosinase Inhibitor assay protocol summary
- Screening compound preparation
- Preparation of controls
- Enzyme and substrate solution preparation
- Add enzyme solution and incubate at 25°C for 10 minutes
- Add substrate solution
- Measure absorbance (OD 510 nm) in a kinetic mode for 30-60 minutes at 25°C

How other researchers are using ab204715
Tyrosinase Inhibitor Assay Kit ab204715 has been used with a variety of sample types including:
- Escherichia coli Salmonella enterica and Pseudomonas aeruginosa1
Highly ripe red guava extracts2
Staphylococcus aureus3
References: 1-Vilas-Boas A et al. 2023, 2-Bao S et al. 2023, 3-Oliveira A et al. 2022

Related and recommended products
To quantify Tyrosinase Activity, we recommend Tyrosinase Activity Assay Kit (Colorimetric) ab252899

Other Notes
This product was previously called K575 Biovison Tyrosinase Inhibitor Screening Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204715-100Test", "assetComponentDetails": [ { "size":"1 x 0.5 mL", "name":"Tyrosinase Enhancer", "number":"AB204715-CMP03", "productcode":"" }, { "size":"1 x 1 Each", "name":"Tyrosine", "number":"AB204715-CMP05", "productcode":"" }, { "size":"1 x 1 Each", "name":"Tyrosinase", "number":"AB204715-CMP04", "productcode":"" }, { "size":"1 x 1 Each", "name":"Kojic Acid", "number":"AB204715-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 42", "number":"AB204715-CMP02", "productcode":"AB323969" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosinase also known as monophenol monooxygenase is a copper-containing enzyme with a mass of approximately 60-70 kDa. It catalyzes the oxidation of phenols such as tyrosine to quinones which are important precursors for melanin synthesis. Tyrosinase is predominantly expressed in melanocytes the cells responsible for pigment production in skin hair and eyes. Its expression also occurs in other tissues like the brain where it might play different roles.
Biological function summary

Within melanin production tyrosinase functions as the rate-limiting enzyme initiating the synthesis path by converting tyrosine into dopaquinone. This enzyme works as part of the melanogenic complex interacting with other enzymes such as tyrosinase-related proteins 1 and 2 to regulate melanin synthesis. The presence and activity of tyrosinase significantly impacts pigmentation processes and skin coloration.

Pathways

Relating tyrosinase to melanin biosynthetic and regulatory pathways reveal its central role. The enzyme is vital in the Raper-Mason pathway for melanin production where it interacts with proteins such as dopachrome tautomerase. It bridges the gap between external stimuli like UV radiation and cellular responses transforming them into pigmentation changes. The L-tyrosine and catecholamine biosynthesis pathways also influence tyrosinase activity linking it to broader metabolic processes.

Issues with tyrosinase can lead to conditions like albinism and melanoma. In oculocutaneous albinism type 1 (OCA1) a genetic mutation in the tyrosinase gene results in reduced or absent enzymatic activity causing hypopigmentation. In melanoma abnormal regulation of tyrosinase and its pathway associates with tumor progression and metastasis often in connection with factors such as microphthalmia-associated transcription factor (MITF) that modulate its expression.
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Product protocols

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Target data

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed : 28661582).
See full target information Tyrosinase
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed40143215

2025

Evaluation of the Anti-Aging Properties of Ethanolic Extracts from Selected Plant Species and Propolis by Enzyme Inhibition Assays and 2D/3D Cell Culture Methods.

Applications

Unspecified application

Species

Unspecified reactive species

F Sezer Senol Deniz,Ilkay Erdogan Orhan,Przemyslaw Andrzej Filipek,Abdulselam Ertas,Ronald Gstir,Thomas Jakschitz,Günther Karl Bonn

International journal of molecular sciences 25: PubMed39596290

2024

Exploring Cannabidiol (CBD) and Cannabigerol (CBG) Safety Profile and Skincare Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Luz-Veiga,Adélia Mendes,Diana Tavares-Valente,Manuela Amorim,António Conde,Manuela Estevez Pintado,Helena R Moreira,João Azevedo-Silva,João Fernandes

International journal of molecular sciences 25: PubMed38203191

2024

Anti-Aging Potential of a Novel Ingredient Derived from Sugarcane Straw Extract (SSE).

Applications

Unspecified application

Species

Unspecified reactive species

Maria João Carvalho,Sílvia Santos Pedrosa,Adélia Mendes,João Azevedo-Silva,João Fernandes,Manuela Pintado,Ana L S Oliveira,Ana Raquel Madureira

Foods (Basel, Switzerland) 12: PubMed38137266

2023

Polyphenol-Rich Extracts Obtained by Conventional, MAE and UAE Methods: Exploring the Bioactive Potential and Safety for Use a Medicine Plant as Food and Nutraceutical Ingredient.

Applications

Unspecified application

Species

Unspecified reactive species

Ana A Vilas-Boas,Ricardo Goméz-García,Manuela Machado,Catarina Nunes,Sónia Ribeiro,João Nunes,Ana L S Oliveira,Manuela Pintado

BioFactors (Oxford, England) 49:1038-1060 PubMed37317790

2023

Skincare potential of a sustainable postbiotic extract produced through sugarcane straw fermentation by Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Duarte,Maria João Carvalho,Nelson Mota de Carvalho,João Azevedo-Silva,Adélia Mendes,Inês Pinto Ribeiro,João Carlos Fernandes,Ana L S Oliveira,Carla Oliveira,Manuela Pintado,Ana Amaro,Ana Raquel Madureira

Molecules (Basel, Switzerland) 28: PubMed36770886

2023

Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System.

Applications

Unspecified application

Species

Unspecified reactive species

Sônia Nair Báo,Manuela Machado,Ana Luisa Da Silva,Adma Melo,Sara Cunha,Sérgio S Sousa,Ana Rita Malheiro,Rui Fernandes,José Roberto S A Leite,Andreanne G Vasconcelos,João Relvas,Manuela Pintado

Foods (Basel, Switzerland) 11: PubMed36553767

2022

Sugarcane Straw Polyphenols as Potential Food and Nutraceutical Ingredient.

Applications

Unspecified application

Species

Unspecified reactive species

Ana L S Oliveira,Maria João Carvalho,Diana Luazi Oliveira,Eduardo Costa,Manuela Pintado,Ana Raquel Madureira

Chemistry & biodiversity 19:e202200272 PubMed35938449

2022

Chemical Composition, Antiaging Activities and Molecular Docking Studies of Essential Oils from Acca sellowiana (Feijoa).

Applications

Unspecified application

Species

Unspecified reactive species

Heba A S El-Nashar,Mai Adel,Mohamed El-Shazly,Ibrahim S Yahia,Hamdy S El Sheshtawy,Adel A Almalki,Nehal Ibrahim

Molecules (Basel, Switzerland) 27: PubMed35565960

2022

A New Xanthone Glycoside from L.: Physicochemical Properties and In Vitro Anti-Skin Aging Activities.

Applications

Unspecified application

Species

Unspecified reactive species

Heba A S El-Nashar,Eman M El-Labbad,Mahmood A Al-Azzawi,Naglaa S Ashmawy

International journal of molecular sciences 21: PubMed32365630

2020

Tyrosinase-Targeting Gallacetophenone Inhibits Melanogenesis in Melanocytes and Human Skin-Equivalents.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Young Lee,Jooyun Lee,Daejin Min,Juewon Kim,Hyoung-June Kim,Kyoung Tai No
View all publications
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