Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Foods (Basel, Switzerland) 12:2023Polyphenol-Rich Extracts Obtained by Conventional, MAE and UAE Methods: Exploring the Bioactive Potential and Safety for Use a Medicine Plant as Food and Nutraceutical Ingredient.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna A Vilas-Boas,Ricardo Goméz-García,Manuela Machado,Catarina Nunes,Sónia Ribeiro,João Nunes,Ana L S Oliveira,Manuela PintadoPubMed 38137266
- BioFactors (Oxford, England) 49:1038-10602023Skincare potential of a sustainable postbiotic extract produced through sugarcane straw fermentation by Saccharomyces cerevisiae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Duarte,Maria João Carvalho,Nelson Mota de Carvalho,João Azevedo-Silva,Adélia Mendes,Inês Pinto Ribeiro,João Carlos Fernandes,Ana L S Oliveira,Carla Oliveira,Manuela Pintado,Ana Amaro,Ana Raquel MadureiraPubMed 37317790
- Molecules (Basel, Switzerland) 28:2023Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSônia Nair Báo,Manuela Machado,Ana Luisa Da Silva,Adma Melo,Sara Cunha,Sérgio S Sousa,Ana Rita Malheiro,Rui Fernandes,José Roberto S A Leite,Andreanne G Vasconcelos,João Relvas,Manuela PintadoPubMed 36770886
- Foods (Basel, Switzerland) 11:2022Sugarcane Straw Polyphenols as Potential Food and Nutraceutical Ingredient.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna L S Oliveira,Maria João Carvalho,Diana Luazi Oliveira,Eduardo Costa,Manuela Pintado,Ana Raquel MadureiraPubMed 36553767
- Chemistry & biodiversity 19:e2022002722022Chemical Composition, Antiaging Activities and Molecular Docking Studies of Essential Oils from Acca sellowiana (Feijoa).Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeba A S El-Nashar,Mai Adel,Mohamed El-Shazly,Ibrahim S Yahia,Hamdy S El Sheshtawy,Adel A Almalki,Nehal IbrahimPubMed 35938449
- Molecules (Basel, Switzerland) 27:2022A New Xanthone Glycoside from L.: Physicochemical Properties and In Vitro Anti-Skin Aging Activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeba A S El-Nashar,Eman M El-Labbad,Mahmood A Al-Azzawi,Naglaa S AshmawyPubMed 35565960
- International journal of molecular sciences 21:2020Tyrosinase-Targeting Gallacetophenone Inhibits Melanogenesis in Melanocytes and Human Skin-Equivalents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Young Lee et. al.PubMed 32365630
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity
Target data
Function
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (By similarity). Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (By similarity).
Alternative names
Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
What's included?
Recommended products
Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of kojic acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The assay is also adaptable to a 384-well format.
This product is manufactured by BioVision, an Abcam company and was previously called K575 Tyrosinase Inhibitor Screening Kit (Colorimetric). K575-100 is the same size as the 100 test size of ab204715.
Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosinase also known as monophenol monooxygenase is a copper-containing enzyme with a mass of approximately 60-70 kDa. It catalyzes the oxidation of phenols such as tyrosine to quinones which are important precursors for melanin synthesis. Tyrosinase is predominantly expressed in melanocytes the cells responsible for pigment production in skin hair and eyes. Its expression also occurs in other tissues like the brain where it might play different roles.
- Biological function summary
Within melanin production tyrosinase functions as the rate-limiting enzyme initiating the synthesis path by converting tyrosine into dopaquinone. This enzyme works as part of the melanogenic complex interacting with other enzymes such as tyrosinase-related proteins 1 and 2 to regulate melanin synthesis. The presence and activity of tyrosinase significantly impacts pigmentation processes and skin coloration.
- Pathways
Relating tyrosinase to melanin biosynthetic and regulatory pathways reveal its central role. The enzyme is vital in the Raper-Mason pathway for melanin production where it interacts with proteins such as dopachrome tautomerase. It bridges the gap between external stimuli like UV radiation and cellular responses transforming them into pigmentation changes. The L-tyrosine and catecholamine biosynthesis pathways also influence tyrosinase activity linking it to broader metabolic processes.
- Associated diseases and disorders
Issues with tyrosinase can lead to conditions like albinism and melanoma. In oculocutaneous albinism type 1 (OCA1) a genetic mutation in the tyrosinase gene results in reduced or absent enzymatic activity causing hypopigmentation. In melanoma abnormal regulation of tyrosinase and its pathway associates with tumor progression and metastasis often in connection with factors such as microphthalmia-associated transcription factor (MITF) that modulate its expression.
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1 product image
Inhibition Assay - Tyrosinase Inhibitor Assay Kit (Colorimetric) (ab204715)
Inhibition of Tyrosinase Enzymatic Activity with Kojic Acid.
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