UGT Activity Assay / Ligand Screening Kit (Fluorometric)

Product details

UGT Activity Assay / Ligand Screening Kit (Fluorometric) (ab273331) enables rapid measurement of native or recombinant UGT activity in biological samples such as liver microsomes and can also be used to assess the effect of drugs and other novel compounds on UGT activity. The assay utilizes a highly fluorescent UGT substrate with a large Stokes shift (Ex/Em = 415/502 nm) that allows determination of UGT activity by tracking the drop in fluorescence emission as the substrate is converted into a non-fluorescent glucuronide. The multi-isozyme substrate is glucuronidated by virtually all of the pharmacologically-relevant mammalian UGT1A and UGT2B enzymes. UGT specific activity is calculated by comparing the fluorescence loss versus a control reaction performed in the absence of the required cofactor UDPGA. The kit includes the pore-forming peptide antibiotic Alamethicin, which allows the UGT Substrate and UDPGA to rapidly diffuse across lipid membranes to access the UGT active site located in the lumen of microsomes. For verification of modulation of UGT activity by test ligands, diclofenac, a competitive inhibitor of most human and rodent UGT isozymes, is also included. The assay is highly sensitive, simple to perform and high-throughput adaptable. This assay can detect less than 0.1 mU UGT activity in biological samples. The kit contains a complete set of reagents sufficient for performing 100 reactions at a 100 μl reaction volume.

Note: The UGT Substrate is glucuronidated by the majority of characterized human hepatic and non-hepatic UGT isozymes, aside from UGT1A4. Thus, the calculated UGT activity represents a composite of all of the isozymes expressed in a particular sample. In human liver microsomes, the major isozymes include UGT1A1, 1A3, 1A6, 1A9 and 2B7.

This product is manufactured by BioVision, an Abcam company and was previously called K692 UGT Activity Assay / Ligand Screening Kit (Fluorometric). K692-100 is the same size as the 100 test size of ab273331.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.