Urea Assay Kit

• Biochemical assay

PubMed

Biochemical assay - Urea Assay Kit (AB83362)

Antar et al. used Urea Assay kit ab83362 to investigate the effect of metformin therapeutic activity on doxorubicin-induced nephrotoxicity .

Bar-dot plot of the prophylactic effect of Met on DOX-induced renal dysfunction. Prophylactic treatment with Met for 3 weeks decreased urea in the DOX-nephrotoxic mice. Student's t-test was used for statistical comparisons. Data are expressed as mean ± SEM. Colored dots indicate each individual value and colored curves represent the distribution of each data point. *** p < 0.001 vs. control group and ### p < 0.001 vs. DOX group.

According to the commercially available kits, urea was assessed in accordance with the manufacturer's instructions (ab83362; Abcam, Cambridge, UK).

• Biochemical assay

Lab

Biochemical assay - Urea Assay Kit (AB83362)

Diagram showing the principles of the Urea assay method.

• Biochemical assay

Supplier Data

Biochemical assay - Urea Assay Kit (AB83362)

Diagram showing the principles of the Urea Assay pathway

Urea initiates a cascade of reactions ultimately reacting with OxiRed, resulting in a colorimetric product with absorbance at 570 nm.

• FuncS

Collaborator

Functional Studies - Urea Assay Kit (AB83362)

Urea measured in cell culture supernatants and control medium (MEM), background signal subtracted (duplicates +/- SD).

The samples were tested neat; 1 : 4; 1 : 16 and 1 : 64. Only the latter two gave reliable results.

• FuncS

Lab

Functional Studies - Urea Assay Kit (AB83362)

Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

• FuncS

Lab

Functional Studies - Urea Assay Kit (AB83362)

Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).

• FuncS

Supplier Data

Functional Studies - Urea Assay Kit (AB83362)

Example of Urea standard curve using ab83362

• FuncS

Lab

Functional Studies - Urea Assay Kit (AB83362)

Urea measured in biological fluids, background signal subtracted (duplicates; +/- SD).

• FuncS

Unknown

Functional Studies - Urea Assay Kit (AB83362)

ab83362 was used as a BUN assay (Blood urea nitrogen assay) to measure urea levels in 80 female Sprague-Dawley rats (age 10-12 months-old).

Howie, R Nicole et al., PloS one: vol. 10,7 e0132520., Fig 6, doi:10.1371/journal.pone.0132520

• Biochemical assay

PubMed

Biochemical assay - Urea Assay Kit (AB83362)

Ramachandra et al. used Urea Assay kit ab83362 to measure urea in brain tissue and to investigate the potential of embelin and levodopa combination therapy for Parkinson’s disease treatment.

Antioxidant potential of embelin (E) and embelin plus LD therapies in mice treated with rotenone, based on the measurement of brain (a) LPO, denoted by MDA, (b) NO, (c) peroxynitrite (PN), and (d) brain urea (BU) levels. The values represent the mean ± standard error (n = 6). For the embelin groups, the number indicates the dose (in mg kg 1 body weight).

• Biochemical assay

PubMed

Biochemical assay - Urea Assay Kit (AB83362)

Aguilar et al. used Urea Assay kit ab83362 to investigate how the carcinogenic bacterium Helicobacter pylori attaches to gastric cells in a gastric organoid cell culture model.

Quantification of urea concentration in gastric organoid-derived monolayer conditioned media. Fold over control differentiation media (grey, ENRWFGTi_) Organoids were cultured in three different types of media : Orange ENRWFGTiNi. Gray ENRWFGTi_. Green ENR_FGTi_.

The urea concentration in the conditioned-organoid media was measured using a Urea Assay kit (ab83362, Abcam) and following the manufacturer’s instructions. Samples were collected after 24 h, centrifuged for 15 min at 1500 x g to remove cell debris, and diluted 1/100 in the urea kit buffer prior to quantification.

• Biochemical assay

PubMed

Biochemical assay - Urea Assay Kit (AB83362)

Jian et al. used Urea Assay kit ab83362 and Albumin ELISA Kit ab179887 to investigate liver function markers in a HepaRG/HUVECs/LX-2-laden invitro liver model.

Hepatic function evaluations of 2D and 3D models. Determination of the total ALB and urea secretions in the supernatant at days 1, 4 and 7 during culture. The asterisks in figure indicate the level of significant difference (*p < 0.05, **p < 0.01).

For hepatic function measurements, the ALB secretion and urea synthesis were quantified using the corresponding assay kits following the protocol from the manufacturer. At days 1, 4, and 7 during culture, the supernatant was collected and stored at -80°C. The insoluble matter of the sample was centrifugally removed prior to testing. ALB and urea secretions in the supernatant were determined using the ELISA kit (ab179887, Abcam) and urea assay kit (ab83362, Abcam), respectively. ALB and urea contents were assayed by absorbance at 450 nm and 462 nm on a microplate reader, respectively.

• Biochemical assay

PubMed

Biochemical assay - Urea Assay Kit (AB83362)

Urea Assay Kit ab83362 used to measure blood urea nitrogen with mouse serum, and Mouse Albumin ELISA kit ab108792 and Creatinine Assay Kit ab65340 used to measure albumin-creatinine ratio with mouse urine.

Widjaja et al subjected IL11 null mice and wild type controls to acute kidney injury and ran a blood urea nitrogen assay (Urea Assay Kit ab83362), and measured urine albumin-creatinine ratio using Mouse Albumin ELISA kit ab108792 and Creatinine Assay Kit ab65340.

Red indicates acute kidney injury. AKI was induced by intraperitoneal (IP) injection of folic acid (FA, 200 mg/kg) in vehicle (0.3 M NaHCO3) to 10–12-week-old male mice; control mice were administered vehicle alone.

All ELISA and colorimetric assays were performed according to the manufacturer’s protocol.

• Biochemical assay

PubMed

Biochemical assay - Urea Assay Kit (AB83362)

Lee et al. used Urea Assay kit ab83362, AST Activity Assay Kit ab105135, and ALT Assay Kit ab105134 to investigate the pathology in various tissues of K18-hACE2 mice after SARS-CoV-2 infection.

Eight-week-old male K18-hACE2 mice (five mice per group) were infected intranasally with 2 x 10³ PFU of SARS-CoV-2 WT (blue symbols) or β variant (B.1.351) (red symbols). Body weight was monitored. Mice were sacrificed at 4 and 6 dpi. Serum and tissue samples were collected. Uninfected mice (-) (black symbols) were used as controls. Multiple-organ damage evaluated by measuring serum markers in WT- and β variant-infected mice at 6 dpi : Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Blood urea nitrogen (BUN). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. NS, not significant. (One-way ANOVA with Tukey's multiple-comparison test was performed.)

Multiple-organ damage was evaluated by determining the levels of serum AST and ALT (markers of liver injury) and BUN (a marker of kidney injury). AST, ALT, and BUN levels were measured using an AST activity assay kit (no. ab105135), ALT assay kit (no. ab105134), and urea assay kit (no. ab83362) (Abcam), respectively, according to the manufacturer's instructions.

• Other

Supplier Data

Other - Urea Assay Kit (AB83362)

Representative image of Urea Assay Kit ab83362

Components shown from left to right :

- OxiRed Probe

- Urea Standard

- Converter Mix D

- Developer Mix A

- Developer Mix L

- Assay Buffer 14

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.