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Urea Assay Kit ab83362 is a no-wash Urea assay, used with bodily fluids, cell lysates, tissue extracts, and cell culture supernatants, with one 60 min incubation and readout on colorimetric (570 nm) microplate reader. Urea is acted on by urease to produce ammonia, and following the action of downstream enzymes in the assay, a colored product is produced.

Convenient Urea assay kit designed for success:
Complete kit format with full Urea assay protocol, and standard curve for quantitation
Cited in over 80 publications
Easy scale up with batch sizes up to 500 kits


Images

Functional Studies - Urea Assay Kit (AB83362), expandable thumbnail
  • Functional Studies - Urea Assay Kit (AB83362), expandable thumbnail
  • Functional Studies - Urea Assay Kit (AB83362), expandable thumbnail
  • Functional Studies - Urea Assay Kit (AB83362), expandable thumbnail
  • Functional Studies - Urea Assay Kit (AB83362), expandable thumbnail

Publications

  • Science advances 10:eadn26892024
    An ultrathin membrane mediates tissue-specific morphogenesis and barrier function in a human kidney chip.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Xingrui Mou,Jessica Shah,Yasmin Roye,Carolyn Du,Samira Musah
    PubMed 38838141
  • Journal of tissue engineering 14:204173142312198132023
    Rat liver extracellular matrix and perfusion bioreactor culture promote human amnion epithelial cell differentiation towards hepatocyte-like cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Sara Campinoti,Bruna Almeida,Negin Goudarzi,Stefan Bencina,Fabio Grundland Freile,Claire McQuitty,Dipa Natarajan,I Jane Cox,Adrien Le Guennec,Vamakshi Khati,Giulia Gaudenzi,Roberto Gramignoli,Luca Urbani
    PubMed 38143931

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Cell Lysate

Assay type

Quantitative

Range

0.5 - 5 nmol/well

Assay time

1h

Sensitivity

> 0.5 nmol/well

Associated Products

Select an associated product type

1 products for Alternative Product

What's included?

2000 Test
Components
Assay Buffer XIV
20 x 25 mL
Converter Enzyme V
20 x 1 Vial
Developer I
20 x 1 Vial
Development Enzyme Mix I
20 x 1 Vial
OxiRed Probe
20 x 200 µL
Urea Standard
20 x 100 µL

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Urea Assay Kit ab83362 is a no-wash Urea assay, used with bodily fluids, cell lysates, tissue extracts, and cell culture supernatants, with one 60 min incubation and readout on colorimetric (570 nm) microplate reader. Urea is acted on by urease to produce ammonia, and following the action of downstream enzymes in the assay, a colored product is produced.

Convenient Urea assay kit designed for success:
Complete kit format with full Urea assay protocol, and standard curve for quantitation
Cited in over 80 publications
Easy scale up with batch sizes up to 500 kits

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Cell Lysate

Assay type

Quantitative

Range

0.5 - 5 nmol/well

Assay time

1h

Assay Platform

Microplate reader

Sensitivity

> 0.5 nmol/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Urea Assay Kit ab83362 is a rapid, simple, sensitive, and reliable assay used to quantify urea in a variety of samples such as serum, plasma, and urine, etc. It can be used as a blood urea nitrogen assay kit (BUN assay kit).

In the urea assay protocol, urea is acted on by enzymes to form a product that reacts with a probe to generate color (ODmax=570nm). The absorbance is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 μM of urea.

Urea assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37°C
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K375 Urea Colorimetric Assay Kit. K375-100 is the same size as the 100 test size of ab83362.

Supplementary info

Activity summary

Urea also known as carbamide is a small molecule with a molecular mass of 60.06 g/mol. It results from protein metabolism and is eliminated by the kidneys. Urea plays a central role in the nitrogen cycle in humans. The liver predominantly expresses it where it is then transported to the kidneys for excretion. Blood urea nitrogen (BUN) and plasma urea levels are measured using assays like the BUN kit and the BUN test Boston to assess kidney function.

Biological function summary

In the context of nitrogen waste management urea serves as an essential substance in detoxifying ammonia. Urea is part of the urea cycle a series of biochemical reactions converting ammonia into urea. Ammonia results from amino acid breakdown and its accumulation can be toxic. The urea cycle ensures safe excretion therefore playing a protective role in maintaining nitrogen balance.

Pathways

The urea cycle is interlinked with the citric acid cycle especially in the liver. It involves critical enzymes such as carbamoyl phosphate synthetase and ornithine transcarbamylase. These pathways share intermediates and energy regulation roles ensuring efficient functioning of nitrogen metabolism. The pathways connect with proteins like arginase which facilitate the final step in the conversion of arginine to urea.

Associated diseases and disorders

Improper urea cycle function leads to conditions such as hyperammonemia and acute renal failure. Elevated blood urea nitrogen levels indicate potential kidney dysfunction. Moreover defects in proteins like arginase or ornithine transcarbamylase can disrupt this balance resulting in urea cycle disorders. Monitoring blood urea levels helps in diagnosing and managing these conditions effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Functional Studies - Urea Assay Kit (ab83362), expandable thumbnail
    Howie, R Nicole et al., PloS one?vol. 10,7 e0132520., Fig 6, doi:10.1371/journal.pone.0132520

    Functional Studies - Urea Assay Kit (ab83362)

    ab83362 was used as a BUN assay (Blood urea nitrogen assay) to measure urea levels in 80 female Sprague-Dawley rats (age 10-12 months-old).

  • Functional Studies - Urea Assay Kit (ab83362), expandable thumbnail

    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in cell culture supernatants and control medium (MEM), background signal subtracted (duplicates +/- SD).

    The samples were tested neat; 1:4; 1:16 and 1:64. Only the latter two gave reliable results.

  • Functional Studies - Urea Assay Kit (ab83362), expandable thumbnail

    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Functional Studies - Urea Assay Kit (ab83362), expandable thumbnail

    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).

  • Functional Studies - Urea Assay Kit (ab83362), expandable thumbnail

    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in biological fluids, background signal subtracted (duplicates; +/- SD).

  • Functional Studies - Urea Assay Kit (ab83362), expandable thumbnail

    Functional Studies - Urea Assay Kit (ab83362)

    Example of Urea standard curve using ab83362

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com