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AB204697

Urease Activity Assay Kit (Colorimetric)

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(5 Publications)

Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to determine urease activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Urease, Jack bean urease, Urea amidohydrolase, JBU

3 Images
Functional Studies - Urease Activity Assay Kit (Colorimetric) (AB204697)
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Supplier Data

Functional Studies - Urease Activity Assay Kit (Colorimetric) (AB204697)

Measurement of Urease activity in soil.

Functional Studies - Urease Activity Assay Kit (Colorimetric) (AB204697)
  • FuncS

Supplier Data

Functional Studies - Urease Activity Assay Kit (Colorimetric) (AB204697)

Typical ammonia Standard calibration curve using Urease Activity Assay Kit (Colorimetric) (ab204697).

Functional Studies - Urease Activity Assay Kit (Colorimetric) (AB204697)
  • FuncS

Supplier Data

Functional Studies - Urease Activity Assay Kit (Colorimetric) (AB204697)

Measurement of Urease activity in Staphylococcus aureus and Saccharomyces cerevisiae (yeast) cell extracts.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Urine, Suspension cells, Adherent cells

Assay type

Enzyme activity

Sensitivity

< 0.001 mU/well

Assay time

1h 10m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.

Urease assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- add additional reagents and incubate for 30 min
- analyze with microplate reader

Other Notes
This product was previously called K378 Biovision Urease Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil samples and fecal matter. Due to ammonia production, urease can play a role in the pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary tract pathogens.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204697-100Test", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Urease Assay Buffer", "number":"AB204697-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix D", "number":"AB204697-CMP04", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"50X Urea Solution", "number":"AB204697-CMP05", "productcode":"" }, { "size":"1 x 4 mL", "name":"Reagent III", "number":"AB204697-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Ammonium Standard II", "number":"AB204697-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Reagent II", "number":"AB204697-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urease also known as urea amidohydrolase is an enzyme with a significant role in nitrogen metabolism. It mechanically catalyzes the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is commonly associated with a molecular mass ranging from 480 kDa to 600 kDa depending on species and isoform. Urease expresses predominantly in bacteria fungi and plants with significant incidence in gastrointestinal tract microorganisms like Helicobacter pylori. It is also present in some invertebrates but not usually in vertebrates.
Biological function summary

Urease plays a significant role in nitrogen metabolism by assisting the recycling of nitrogenous waste products and facilitating nutrient acquisition. It functions mainly as part of a multi-subunit complex usually composed of alpha beta and gamma subunits. These subunits coordinate to form a metalloenzyme that requires nickel ions to be catalytically active. This enzyme's ability to convert urea into ammonia makes it essential for nitrogen economy in various ecological settings.

Pathways

Urease is an integral component of the nitrogen cycle and is active in the urea degradation pathway. This pathway is important for recycling nitrogen in nature affecting soil fertility and plant growth. Urease links to proteins involved in ammonia assimilation such as glutamine synthetase which helps incorporate ammonia into amino acids. This relationship facilitates nitrogen availability for living organisms and enhances ecosystem productivity.

Urease relates to conditions like gastric ulcers and certain kidney stones. Helicobacter pylori employs urease to neutralize stomach acid enabling its survival and leading to gastritis and peptic ulcer disease. Also urease activity contributes to the formation of struvite stones in the urinary tract where urea-splitting bacteria like Proteus mirabilis produce ammonia leading to stone formation. These conditions illustrate urease's involvement with bacterial proteins like flagellar proteins and virulence factors that facilitate colonization and infection.
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Product protocols

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Target data

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism (PubMed : 26690979). Is known to be highly toxic and lethal when given by intravenous route, producing convulsions and other signs of central nervous system intoxication associated with the high levels of ammonia formed in the blood of mice and rabbits (PubMed : 26690979). Is neurotoxic in mammals, when directly injected into hippocampus (PubMed : 33631299). It may induce seizures by acting at a neuronal network level, thereby disturbing electroencephalographic rhythms and causing metabolic alterations in key areas related to epileptogenesis and to neurogenic pulmonary edema (PubMed : 33631299). It increases calcium influx and neuronal firing rate in the hippocampus (PubMed : 33631299). Is able to insert itself into lipid bilayers, altering physicochemical properties of artificial membranes, and forming cation-selective ion channels (PubMed : 24583269). In vitro, has the ability to induce platelet aggregation, platelet granules secretion and release of ATP (PubMed : 11696010). In contrast to canatoxin, another urease from C.ensiformis, is not lethal to mice when intraperitoneally injected (PubMed : 11696010).
See full target information Urease
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Environmental science and pollution research international : PubMed40011333

2025

Application of Sporosarcina pasteurii for the biomineralization of calcite in the treatment of waste concrete fines.

Applications

Unspecified application

Species

Unspecified reactive species

Kristyna Klikova,Petr Holecek,Vaclav Nezerka,Zdenek Prosek,Dana Konakova,Katerina Demnerova,Hana Stiborova

Animal bioscience 35:1592-1605 PubMed35468273

2022

Three-step in vitro digestion model for evaluating and predicting fecal odor emission from growing pigs with different dietary protein intakes.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Hua Lo,Ching-Yi Chen,Han-Tsung Wang

Frontiers in microbiology 11:596681 PubMed33414773

2020

Functional Changes of the Community of Microbes With Ni-Dependent Enzyme Genes Accompany Adaptation of the Ruminal Microbiome to Urea-Supplemented Diets.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyan Lu,Zhihui Xu,Lingmeng Kong,Hong Shen,Jörg R Aschenbach

Frontiers in microbiology 11:573907 PubMed33193181

2020

Diverse Enzymes With Industrial Applications in Four Thraustochytrid Genera.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiu-Chin Lin,Wei-Hao Li,Chi-Chih Chen,Tien-Hsing Cheng,Yu-Hsuan Lan,Ming-Der Huang,Wen-Ming Chen,Jo-Shu Chang,Hsin-Yang Chang

Frontiers in microbiology 10:2417 PubMed31736886

2019

Rewiring of Metabolic Network in During Adaptation to Different Stresses.

Applications

Unspecified application

Species

Unspecified reactive species

Arshad Rizvi,Arvind Shankar,Ankita Chatterjee,Tushar H More,Tungadri Bose,Anirban Dutta,Kannan Balakrishnan,Lavanya Madugulla,Srikanth Rapole,Sharmila S Mande,Sharmistha Banerjee,Shekhar C Mande
View all publications
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