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AB65344

Uric Acid Assay Kit

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(38 Publications)

Uric Acid Assay Kit ab65344 provides a convenient means for detecting uric acid in biological samples such as serum and urine.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
5 Images
Functional Studies - Uric Acid Assay Kit (AB65344)
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Lab

Functional Studies - Uric Acid Assay Kit (AB65344)

Uric acid measured in biological fluid (duplicates; +/- SD).

Functional Studies - Uric Acid Assay Kit (AB65344)
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Unknown

Functional Studies - Uric Acid Assay Kit (AB65344)

Example of Fluormetic Standard Curve.

Uric acid fluorimetric standard curve.

Functional Studies - Uric Acid Assay Kit (AB65344)
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Lab

Functional Studies - Uric Acid Assay Kit (AB65344)

Uric acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (duplicates; +/- SD).

Functional Studies - Uric Acid Assay Kit (AB65344)
  • FuncS

Supplier Data

Functional Studies - Uric Acid Assay Kit (AB65344)

Uric acid colorimetric standard curve.

Other - Uric Acid Assay Kit (AB65344)
  • Other

Supplier Data

Other - Uric Acid Assay Kit (AB65344)

Representative image of Uric Acid Assay Kit ab65344

Components shown from left to right :

- OxiRed Probe

- Uric Acid Standard

- Uric Acid Enzyme Mix

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Assay time

40m

Assay Platform

Microplate reader

Product details

Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.

Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.

Uric acid assay protocol summary:
- add reaction mix to samples and standards
- incubate for 30 min
- analyze on microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K608 Uric Acid Colorimetric/Fluorometric Assay Kit. K608-100 is the same size as the 100 test size of ab65344.

Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65344-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Uric Acid Standard", "number":"AB65344-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Uric Acid Enzyme Mix", "number":"AB65344-CMP02", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65344-CMP03", "productcode":"AB309437" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB65344-CMP01", "productcode":"AB136652" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uric acid a compound with a molecular mass of approximately 168.11 g/mol is an end product of purine metabolism in humans and other primates. It forms primarily in the liver and is then transported via the bloodstream to the kidneys where it is excreted in urine. This small nitrogenous waste product serves as a marker of kidney function and metabolic health but it is not a protein. Uric acid exists in higher concentrations in the blood and urine and can crystallize when concentrations exceed its solubility leading to disorders such as gout.
Biological function summary

Uric acid plays a negligible role outside its status as a metabolic product. It does not participate in any known physiological complexes but serves as an antioxidant in the bloodstream neutralizing free radicals and potentially preventing cellular damage. Despite this its protective benefits are overshadowed by its tendency to cause issues when accumulated in high concentrations forming crystals in joints and tissues.

Pathways

Uric acid creation involves the degradation of purine nucleotides through a multi-step enzymatic process. Xanthine oxidase an important enzyme in these pathways converts hypoxanthine to xanthine and then to uric acid. This pathway overlaps with the salvage pathway which includes the enzyme HGPRT (hypoxanthine-guanine phosphoribosyltransferase) involved in recycling purines highlighting the balance between synthesis and degradation of nucleotides.

Elevated levels of uric acid are connected to gout a type of inflammatory arthritis characterized by sudden severe pain redness and swelling in the joints. This condition arises from the deposition of monosodium urate crystals particularly in joints and surrounding tissues. Uric acid levels also link to kidney stones where excess urate accumulates leading to renal complications. Proteins such as ABCG2 (ATP-binding cassette sub-family G member 2) influence the excretion of uric acid and mutations in these proteins can contribute to hyperuricemia and related disorders.

Product protocols

Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40399065

2025

Inhibition of xanthine oxidoreductase with febuxostat, but not allopurinol, prevents inflammasome assembly and IL-1β release.

Applications

Unspecified application

Species

Unspecified reactive species

Lauar de Brito Monteiro,Anne-Sophie Archambault,Lucas F Starchuk,Armando Alcazar,Ju Hee Oh,Joshua A Dubland,Bojana Rakić,Annette E Patterson,C Bruce Verchere,Ramon I Klein Geltink

Frontiers in cell and developmental biology 13:1539496 PubMed40109359

2025

Evaluating oxidative stress targeting treatments in models of placental stress relevant to preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Dinara Afrose,Matt D Johansen,Valentina Nikolic,Natasa Karadzov Orlic,Zeljko Mikovic,Milan Stefanovic,Zoran Cakic,Philip M Hansbro,Lana McClements

Nature communications 15:9729 PubMed39523369

2024

Base-editing corrects metabolic abnormalities in a humanized mouse model for glycogen storage disease type-Ia.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Arnaoutova,Yvonne Aratyn-Schaus,Lisa Zhang,Michael S Packer,Hung-Dar Chen,Cheol Lee,Sudeep Gautam,Francine M Gregoire,Dominique Leboeuf,Steven Boule,Thomas P Fernandez,Victoria Huang,Lo-I Cheng,Genesis Lung,Brianna Bannister,Jeremy Decker,Thomas Leete,Lan S Shuang,Caroline Bock,Prachi Kothiyal,Phil Grayson,Ka W Mok,Jeffrey J Quinn,Lauren Young,Luis Barrera,Giuseppe Ciaramella,Brian C Mansfield,Janice Y Chou

Journal of translational medicine 22:983 PubMed39482719

2024

Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition.

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Unspecified application

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Unspecified reactive species

Zehao Zhuang,Ao Liu,Jinghong Zhang,Shuangjian Han,Lu Tang,Tingting Yu,Yiping Shi,Hui Li,Heng Yang,Peiyuan Bai,Yanhua Tang

Toxicological research 40:97-110 PubMed38223678

2024

Naringenin suppresses aluminum-induced experimental hepato-nephrotoxicity in mice through modulation of oxidative stress and inflammation.

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Unspecified application

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Unspecified reactive species

Ravina Rai,Zaved Ahmad,Subodh Kumar Jain,Deepali Jat,Siddhartha Kumar Mishra

Nephron 148:333-344 PubMed37839409

2023

Penehyclidine Hydrochloride Improves Rhabdomyolysis-Mediated Acute Kidney Injury by Inhibiting Ferroptosis through the HIF-1α/MT1G Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,ShaSha Luo,HongBao Tan

Heliyon 9:e18069 PubMed37483701

2023

Evaluation of anti-hyperuricemic effects of Miq. (Keladi Candik) extracts in potassium oxonate induced rat model.

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Unspecified application

Species

Unspecified reactive species

Ferid Abdulhafiz,Arifullah Mohammed,Mohd Farhan Hanif Reduan,Zulhazman Hamzah,Zulhisyam Abdul Kari,Guillermo Téllez-Isaías

PLoS neglected tropical diseases 17:e0011164 PubMed36920999

2023

Increased hepatic interleukin-1, arachidonic acid, and reactive oxygen species mediate the protective potential of peptides shared by gut cysteine peptidases against Schistosoma mansoni infection in mice.

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Hatem Tallima,Rashika El Ridi

Nutrients 15: PubMed36839190

2023

Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets.

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Unspecified application

Species

Unspecified reactive species

Qing Li,Yushi Du,Panyin Xiang,Guanyu Chen,Xiaoxian Qian,Shuangshuang Li,Yihui Mao,Wenhua Ling,Dongliang Wang

Current issues in molecular biology 45:1183-1196 PubMed36826023

2023

Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy S Younis
View all publications
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