Uric Acid Assay Kit ab65344 provides a convenient means for detecting uric acid in biological samples such as serum and urine.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nephron 148:333-3442023Penehyclidine Hydrochloride Improves Rhabdomyolysis-Mediated Acute Kidney Injury by Inhibiting Ferroptosis through the HIF-1α/MT1G Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,ShaSha Luo,HongBao TanPubMed 37839409
- Heliyon 9:e180692023Evaluation of anti-hyperuricemic effects of Miq. (Keladi Candik) extracts in potassium oxonate induced rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFerid Abdulhafiz,Arifullah Mohammed,Mohd Farhan Hanif Reduan,Zulhazman Hamzah,Zulhisyam Abdul Kari,Guillermo Téllez-IsaíasPubMed 37483701
- PLoS neglected tropical diseases 17:e00111642023Increased hepatic interleukin-1, arachidonic acid, and reactive oxygen species mediate the protective potential of peptides shared by gut cysteine peptidases against Schistosoma mansoni infection in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHatem Tallima,Rashika El RidiPubMed 36920999
- Nutrients 15:2023Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Li,Yushi Du,Panyin Xiang,Guanyu Chen,Xiaoxian Qian,Shuangshuang Li,Yihui Mao,Wenhua Ling,Dongliang WangPubMed 36839190
- Current issues in molecular biology 45:1183-11962023Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNancy S YounisPubMed 36826023
- BMC complementary medicine and therapies 22:2782022Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangxing Shui,Zheyi Cai,Feng Wang,Ting Chen,Xueyuan Huang,Yun Cai,Xiuhua MiPubMed 36271349
- Scientific reports 12:140562022Effects of Thai native chicken breast meat consumption on serum uric acid level, biochemical parameters, and antioxidant activities in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrapassorn Potue,Petcharat Chiangsaen,Putcharawipa Maneesai,Juthamas Khamseekaew,Poungrat Pakdeechote,Vibuntita Chankitisakul,Wuttigrai Boonkum,Natthaya Duanghaklang,Monchai DuangjindaPubMed 35982129
- Antioxidants (Basel, Switzerland) 11:2022Renal Ischemia/Reperfusion Mitigation via Geraniol: The Role of Nrf-2/HO-1/NQO-1 and TLR2,4/MYD88/NFκB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaged E Mohamed,Mohammad A Elmorsy,Nancy S YounisPubMed 36009287
- Brain sciences 12:2022Uric Acid and Cortisol Levels in Plasma Correlate with Pre-Competition Anxiety in Novice Athletes of Combat Sports.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuis Fernando Garcia de Oliveira,Tácito Pessoa Souza-Junior,Juliane Jellmayer Fechio,José Alberto Fernandes Gomes-Santos,Ricardo Camões Sampaio,Cristina Vasconcelos Vardaris,Rafael Herling Lambertucci,Marcelo Paes de BarrosPubMed 35741598
- Nature immunology 23:543-5552022Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunfeng Li,Audrey Lee,Lilit Grigoryan,Prabhu S Arunachalam,Madeleine K D Scott,Meera Trisal,Florian Wimmers,Mrinmoy Sanyal,Payton A Weidenbacher,Yupeng Feng,Julia Z Adamska,Erika Valore,Yanli Wang,Rohit Verma,Noah Reis,Diane Dunham,Ruth O'Hara,Helen Park,Wei Luo,Alexander D Gitlin,Peter Kim,Purvesh Khatri,Kari C Nadeau,Bali PulendranPubMed 35288714
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Assay time
40m
Target data
What's included?
Recommended products
Uric Acid Assay Kit ab65344 provides a convenient means for detecting uric acid in biological samples such as serum and urine.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Assay time
40m
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.
Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
Uric acid assay protocol summary:
- add reaction mix to samples and standards
- incubate for 30 min
- analyze on microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K608 Uric Acid Colorimetric/Fluorometric Assay Kit. K608-100 is the same size as the 100 test size of ab65344.
Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uric acid a compound with a molecular mass of approximately 168.11 g/mol is an end product of purine metabolism in humans and other primates. It forms primarily in the liver and is then transported via the bloodstream to the kidneys where it is excreted in urine. This small nitrogenous waste product serves as a marker of kidney function and metabolic health but it is not a protein. Uric acid exists in higher concentrations in the blood and urine and can crystallize when concentrations exceed its solubility leading to disorders such as gout.
- Biological function summary
Uric acid plays a negligible role outside its status as a metabolic product. It does not participate in any known physiological complexes but serves as an antioxidant in the bloodstream neutralizing free radicals and potentially preventing cellular damage. Despite this its protective benefits are overshadowed by its tendency to cause issues when accumulated in high concentrations forming crystals in joints and tissues.
- Pathways
Uric acid creation involves the degradation of purine nucleotides through a multi-step enzymatic process. Xanthine oxidase an important enzyme in these pathways converts hypoxanthine to xanthine and then to uric acid. This pathway overlaps with the salvage pathway which includes the enzyme HGPRT (hypoxanthine-guanine phosphoribosyltransferase) involved in recycling purines highlighting the balance between synthesis and degradation of nucleotides.
- Associated diseases and disorders
Elevated levels of uric acid are connected to gout a type of inflammatory arthritis characterized by sudden severe pain redness and swelling in the joints. This condition arises from the deposition of monosodium urate crystals particularly in joints and surrounding tissues. Uric acid levels also link to kidney stones where excess urate accumulates leading to renal complications. Proteins such as ABCG2 (ATP-binding cassette sub-family G member 2) influence the excretion of uric acid and mutations in these proteins can contribute to hyperuricemia and related disorders.
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4 product images
Functional Studies - Uric Acid Assay Kit (ab65344)
Uric acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (duplicates; +/- SD).
Functional Studies - Uric Acid Assay Kit (ab65344)
Uric acid measured in biological fluid (duplicates; +/- SD).
Functional Studies - Uric Acid Assay Kit (ab65344)
Example of Fluormetic Standard Curve.
Uric acid fluorimetric standard curve.
Functional Studies - Uric Acid Assay Kit (ab65344)
Uric acid colorimetric standard curve.
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