Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
- FuncS
Lab
Functional Studies - Uric Acid Assay Kit (AB65344)
Uric acid measured in biological fluid (duplicates; +/- SD).
- FuncS
Unknown
Functional Studies - Uric Acid Assay Kit (AB65344)
Example of Fluormetic Standard Curve.
Uric acid fluorimetric standard curve.
- FuncS
Lab
Functional Studies - Uric Acid Assay Kit (AB65344)
Uric acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (duplicates; +/- SD).
- FuncS
Supplier Data
Functional Studies - Uric Acid Assay Kit (AB65344)
Uric acid colorimetric standard curve.
- Other
Supplier Data
Other - Uric Acid Assay Kit (AB65344)
Representative image of Uric Acid Assay Kit ab65344
Components shown from left to right :
- OxiRed Probe
- Uric Acid Standard
- Uric Acid Enzyme Mix
- Assay Buffer 2
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.
Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
Uric acid assay protocol summary:
- add reaction mix to samples and standards
- incubate for 30 min
- analyze on microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K608 Uric Acid Colorimetric/Fluorometric Assay Kit. K608-100 is the same size as the 100 test size of ab65344.
Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Uric acid plays a negligible role outside its status as a metabolic product. It does not participate in any known physiological complexes but serves as an antioxidant in the bloodstream neutralizing free radicals and potentially preventing cellular damage. Despite this its protective benefits are overshadowed by its tendency to cause issues when accumulated in high concentrations forming crystals in joints and tissues.
Pathways
Uric acid creation involves the degradation of purine nucleotides through a multi-step enzymatic process. Xanthine oxidase an important enzyme in these pathways converts hypoxanthine to xanthine and then to uric acid. This pathway overlaps with the salvage pathway which includes the enzyme HGPRT (hypoxanthine-guanine phosphoribosyltransferase) involved in recycling purines highlighting the balance between synthesis and degradation of nucleotides.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (38)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com