Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay
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(1 Publication)
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix.
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Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU
- FuncS
Supplier Data
Functional Studies - Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay (AB108916)
uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)
- FuncS
Supplier Data
Functional Studies - Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay (AB108916)
uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)
Reactivity data
Product details
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
ab108916 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The amidolytic activity of uPA is quantitated using a highly specific uPA substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the uPA enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Supplementary information
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Biological function summary
Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.
Pathways
Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.
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Publications (1)
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PloS one 17:e0273811 PubMed36174075
2022
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AB108915
Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit
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