Skip to main content

Von Kossa Stain Kit (Calcium Stain) ab150687 is used for staining calcium deposits in paraffin sections.

- Based on modified silver staining method
- Ideal for studying bone tissue and calcification
- Cited in over 50 publications

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687), expandable thumbnail

Publications

Associated Products

Select an associated product type

6 products for Alternative Product

Alternative names

What's included?

100 Test
Components
Nuclear Fast Red Solution
1 x 125 mL
Silver Nitrate Solution
1 x 125 mL
Sodium Thiosulfate Solution (5%)
1 x 125 mL

Recommended products

Von Kossa Stain Kit (Calcium Stain) ab150687 is used for staining calcium deposits in paraffin sections.

- Based on modified silver staining method
- Ideal for studying bone tissue and calcification
- Cited in over 50 publications

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

The Von Kossa (Calcium Stain) is intended for use in the histological visualization of calcium deposits in paraffin sections. This method is not specific for calcium itself but tissues are treated with a silver nitrate solution and the silver is deposited by replacing the calcium reduced by the strong light, and so can be visualized as metallic silver.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation
Calcium in mass deposits Black
Calcium in dispersed deposits Gray
NucleiRed
CytoplasmLight Pink

Control Tissue: Any paraffin-embedded tissue that contains calcium deposits.

To prepare bone section without decalcification, the resin is recommended instead of paraffin.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.

Biological function summary

Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.

Pathways

Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.

Associated diseases and disorders

Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (ab150687), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (ab150687)

    Staining of Bone using ab150687 - Calcium Stain Kit (Modified Von Kossa).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (ab150687), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (ab150687)

    ab150687 Von Kossa Stain Kit (Calcium Stain) staining formalin-fixed-paraffin embedded human liver.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com