Von Kossa Stain Kit (Calcium Stain) ab150687 is used for staining calcium deposits in paraffin sections.
- Based on modified silver staining method
- Ideal for studying bone tissue and calcification
- Cited in over 50 publications
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- iScience 27:1088572024Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Wang,Chaoran Zhao,Jiahuan Zhang,Shengfa Li,Youming Zhang,Yan Gong,Yingyue Zhou,Lei Yan,Sheng Zhang,Zhongmin Zhang,Hongling Hu,Anling Liu,Xiaochun Bai,Zhipeng ZouPubMed 38303710
- Frontiers in endocrinology 14:13086042024Engineering three-dimensional bone macro-tissues by guided fusion of cell spheroids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVinothini Prabhakaran,Ferry P W Melchels,Lyndsay M Murray,Jennifer Z PaxtonPubMed 38169965
- Cells 12:2023Osteopontin Activation and Microcalcification in Venous Grafts Can Be Modulated by Dexamethasone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiam W McQueen,Shameem S Ladak,Georgia R Layton,Kerry Wadey,Sarah J George,Gianni D Angelini,Gavin J Murphy,Mustafa ZakkarPubMed 37998362
- JCI insight 8:2023Myocardial fibrosis and calcification are attenuated by microRNA-129-5p targeting Asporin and Sox9 in cardiac fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLejla Medzikovic,Laila Aryan,Grégoire Ruffenach,Min Li,Nicoletta Savalli,Wasila Sun,Shervin Sarji,Jason Hong,Salil Sharma,Riccardo Olcese,Gregory Fishbein,Mansoureh EghbaliPubMed 37154157
- Frontiers in cell and developmental biology 11:11588122023Kefir peptides attenuate atherosclerotic vascular calcification and osteoporosis in atherogenic diet-fed knockout mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGary Ro-Lin Chang,Wei-Yuan Cheng,Hueng-Chuen Fan,Hsiao-Ling Chen,Ying-Wei Lan,Ming-Shan Chen,Chih-Ching Yen,Chuan-Mu ChenPubMed 37091976
- American journal of physiology. Heart and circulatory physiology 324:H443-H4602023Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYijia Li,Hajime Kubo,Daohai Yu,Yijun Yang,Jaslyn P Johnson,Deborah M Eaton,Remus M Berretta,Michael Foster,Timothy A McKinsey,Jun Yu,John W Elrod,Xiongwen Chen,Steven R HouserPubMed 36763506
- Cell biology and toxicology 39:2665-26842023Herpud1 deficiency alleviates homocysteine-induced aortic valve calcification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqing Xie,Yue Shan,Zhuonan Wu,Nan Liu,Jinjin Yang,Hanlin Zhang,Shiming Sun,Jufang Chi,Weizhong Feng,Hui Lin,Hangyuan GuoPubMed 36746840
- Biomedicines 11:2023Micro Ribonucleic Acid-29a (miR-29a) Antagonist Normalizes Bone Metabolism in Osteogenesis Imperfecta (OI) Mice Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJih-Yang Ko,Feng-Sheng Wang,Sung-Hsiung Chen,Shu-Jui KuoPubMed 36831000
- Frontiers in molecular neuroscience 16:10737232023T-cell infiltration in the central nervous system and their association with brain calcification in -deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang,Yaqiong Ren,Yueni Zhang,Ying Li,Chao Xu,Ziyue Peng,Ying Jia,Shupei Qiao,Zitong Zhang,Lei ShiPubMed 36741925
- Frontiers in physiology 13:10372302022The mechanosensitive Piezo1 channels contribute to the arterial medial calcification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLászló Szabó,Norbert Balogh,Andrea Tóth,Ágnes Angyal,Mónika Gönczi,Dávid Máté Csiki,Csaba Tóth,Ildikó Balatoni,Viktória Jeney,László Csernoch,Beatrix DienesPubMed 36439266
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Alternative names
Ca++, Ca2+
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Von Kossa Stain Kit (Calcium Stain) ab150687 is used for staining calcium deposits in paraffin sections.
- Based on modified silver staining method
- Ideal for studying bone tissue and calcification
- Cited in over 50 publications
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Von Kossa (Calcium Stain) is intended for use in the histological visualization of calcium deposits in paraffin sections. This method is not specific for calcium itself but tissues are treated with a silver nitrate solution and the silver is deposited by replacing the calcium reduced by the strong light, and so can be visualized as metallic silver.
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Staining Interpretation| Calcium in mass deposits | Black |
| Calcium in dispersed deposits | Gray |
| Nuclei | Red |
| Cytoplasm | Light Pink |
Control Tissue: Any paraffin-embedded tissue that contains calcium deposits.
To prepare bone section without decalcification, the resin is recommended instead of paraffin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.
- Biological function summary
Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.
- Pathways
Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.
- Associated diseases and disorders
Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (ab150687)
Staining of Bone using ab150687 - Calcium Stain Kit (Modified Von Kossa).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (ab150687)
ab150687 Von Kossa Stain Kit (Calcium Stain) staining formalin-fixed-paraffin embedded human liver.
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