AB150687

Von Kossa Stain Kit (Calcium Stain)

Name: Von Kossa Stain Kit (Calcium Stain) (ab150687) | Abcam
Brand: Abcam Limited
SKU: AB150687
Price: 150 USD
Availability: InStock

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(56 Publications)

Von Kossa Stain Kit (Calcium Stain) ab150687 is used for staining calcium deposits in paraffin sections.

- Based on modified silver staining method
- Ideal for studying bone tissue and calcification
- Cited in over 50 publications

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687)

Staining of Bone using ab150687 - Calcium Stain Kit (Modified Von Kossa).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687)

ab150687 Von Kossa Stain Kit (Calcium Stain) staining formalin-fixed-paraffin embedded human liver.

Key facts

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Product details

The Von Kossa (Calcium Stain) is intended for use in the histological visualization of calcium deposits in paraffin sections. This method is not specific for calcium itself but tissues are treated with a silver nitrate solution and the silver is deposited by replacing the calcium reduced by the strong light, and so can be visualized as metallic silver.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation

Calcium in mass deposits	Black
Calcium in dispersed deposits	Gray
Nuclei	Red
Cytoplasm	Light Pink

Control Tissue: Any paraffin-embedded tissue that contains calcium deposits.

To prepare bone section without decalcification, the resin is recommended instead of paraffin.

Other Notes

Please note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.

Biological function summary

Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.

Pathways

Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.

Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.

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Product protocols

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Target data

See full target information Calcium

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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:68 PubMed39917001

2025

N-acetyltransferase 10 regulates UNC-51-like kinase 1 to reduce tubular cell injury and kidney stone formation.

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Le Wang,Jinjing Huang,Lei Song,Ben Ke

Cellular and molecular life sciences : CMLS 82:13 PubMed39719538

2024

Trimethylamine-N-oxide accelerates osteoporosis by PERK activation of ATF5 unfolding.

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Yu-Han Lin,Wei-Shiung Lian,Re-Wen Wu,Yu-Shan Chen,Shin-Long Wu,Jih-Yang Ko,Shao-Yu Wang,Holger Jahr,Feng-Sheng Wang

International journal of molecular sciences 25: PubMed39408839

2024

NLRP3 Inflammasome in the Pathogenesis of Miscarriages.

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Wioleta Justyna Omeljaniuk,Marzena Garley,Anna Pryczynicz,Joanna Motyka,Angelika Edyta Charkiewicz,Elżbieta Milewska,Piotr Laudański,Wojciech Miltyk

Nature biomedical engineering 8:1285-1307 PubMed39085645

2024

Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor.

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Vincentius Jeremy Suhardi,Anastasia Oktarina,Mohammed Hammad,Yingzhen Niu,Qingdian Li,Andrew Thomson,Juan Lopez,Jason McCormick,Ugur M Ayturk,Matthew B Greenblatt,Lionel B Ivashkiv,Mathias P G Bostrom,Xu Yang

iScience 27:108857 PubMed38303710

2024

Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9.

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Ting Wang,Chaoran Zhao,Jiahuan Zhang,Shengfa Li,Youming Zhang,Yan Gong,Yingyue Zhou,Lei Yan,Sheng Zhang,Zhongmin Zhang,Hongling Hu,Anling Liu,Xiaochun Bai,Zhipeng Zou

Frontiers in endocrinology 14:1308604 PubMed38169965

2024

Engineering three-dimensional bone macro-tissues by guided fusion of cell spheroids.

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Vinothini Prabhakaran,Ferry P W Melchels,Lyndsay M Murray,Jennifer Z Paxton

Cells 12: PubMed37998362

2023

Osteopontin Activation and Microcalcification in Venous Grafts Can Be Modulated by Dexamethasone.

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Liam W McQueen,Shameem S Ladak,Georgia R Layton,Kerry Wadey,Sarah J George,Gianni D Angelini,Gavin J Murphy,Mustafa Zakkar

JCI insight 8: PubMed37154157

2023

Myocardial fibrosis and calcification are attenuated by microRNA-129-5p targeting Asporin and Sox9 in cardiac fibroblasts.

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Lejla Medzikovic,Laila Aryan,Grégoire Ruffenach,Min Li,Nicoletta Savalli,Wasila Sun,Shervin Sarji,Jason Hong,Salil Sharma,Riccardo Olcese,Gregory Fishbein,Mansoureh Eghbali

Frontiers in cell and developmental biology 11:1158812 PubMed37091976

2023

Kefir peptides attenuate atherosclerotic vascular calcification and osteoporosis in atherogenic diet-fed knockout mice.

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Gary Ro-Lin Chang,Wei-Yuan Cheng,Hueng-Chuen Fan,Hsiao-Ling Chen,Ying-Wei Lan,Ming-Shan Chen,Chih-Ching Yen,Chuan-Mu Chen

American journal of physiology. Heart and circulatory physiology 324:H443-H460 PubMed36763506

2023

Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype.

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Unspecified application

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Yijia Li,Hajime Kubo,Daohai Yu,Yijun Yang,Jaslyn P Johnson,Deborah M Eaton,Remus M Berretta,Michael Foster,Timothy A McKinsey,Jun Yu,John W Elrod,Xiongwen Chen,Steven R Houser

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Von Kossa Stain Kit (Calcium Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Kossa Stain Kit (Calcium Stain) (AB150687)

Key facts

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Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (56)

Product promise