Wound Healing Assay Kit ab242285 contains 2 x 24-well plates each containing 12 proprietary treated plastic inserts.
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- Pharmaceutics 16:2024Innovative Multilayer Electrospun Patches for the Slow Release of Natural Oily Extracts as Dressings to Boost Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoemi Fiaschini,Fiorella Carnevali,Stephen Andrew Van der Esch,Roberta Vitali,Mariateresa Mancuso,Maria Sulli,Gianfranco Diretto,Anna Negroni,Antonio RinaldiPubMed 38399220
- Molecular reproduction and development 90:758-7702023lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in pre-eclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengyun Wan,Jia Huang,Wenting Liu,Xiaoyan Su,Bei Zhao,Xianggang Wang,Lu ZhaoPubMed 37548351
- BioFactors (Oxford, England) 49:1158-11732023SUMO2/3 promotes the progression and oxaliplatin resistance of colorectal cancer through facilitating the SUMOylation at Ku80-K307.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Feng,Jinsong He,Min Yuan,Qing Chen,Xi Zeng,Qilin Zhou,Jian Wu,Bin HanPubMed 37338025
- Diabetic medicine : a journal of the British Diabetic Association 40:e151252023The interface targeting hnRNPA2B1 regulates the repression of transthyretin against human retinal microvascular endothelial cells in high-glucose environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYikun Tian,Lu Ye,Jun Shao,Yu XinPubMed 37186403
- Scientific reports 12:178562022Co-alterations of circadian clock gene transcripts in human placenta in preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoli Zhou,Emily Winn,Duong Nguyen,Eric P Kasten,Margaret G Petroff,Hanne M HoffmannPubMed 36284122
- Science advances 8:eabq82972022loss transforms -deficient cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorea Valcarcel-Jimenez,Connor Rogerson,Cissy Yong,Christina Schmidt,Ming Yang,Monica Cremades-Rodelgo,Victoria Harle,Victoria Offord,Kim Wong,Ariane Mora,Alyson Speed,Veronica Caraffini,Maxine Gia Binh Tran,Eamonn R Maher,Grant D Stewart,Sakari Vanharanta,David J Adams,Christian FrezzaPubMed 36269833
- Annals of translational medicine 9:16242021Hyperglycemia-induced increasing of /circ_0008590 in NF-κB pathway is repressed by miR-1243 in human retinal microvascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Shao,Jiping Cai,Yong Yao,Hui ZhuPubMed 34926668
- Biomedicines 9:2021Exonic Variant rs9479297 Contributes to Concurrent Hepatocellular and Transitional Cell Carcinoma Double Primary Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-De Chu,Kwong-Ming Kee,Wey-Ran Lin,Ming-Wei Lai,Sheng-Nan Lu,Wen-Hung Chung,See-Tong Pang,Chau-Ting YehPubMed 34944636
- Aging 12:8549-85642020Exosomal miRNA-34 from cancer-associated fibroblasts inhibits growth and invasion of gastric cancer cells and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Shi et. al.PubMed 32391804
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Adherent cells
- Assay type
Cell-based
What's included?
Recommended products
Wound Healing Assay Kit ab242285 contains 2 x 24-well plates each containing 12 proprietary treated plastic inserts.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Adherent cells
- Assay type
Cell-based
- Assay Platform
Microscope
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
Multi
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Wound Healing Assay Kit (ab242285) contains 2 x 24-well plates each containing 12 proprietary treated plastic inserts. The inserts create a wound field with a defined gap of 0.9 mm for measuring the migratory and proliferation rates of cells. Migratory cells are able to extend protrusions and ultimately invade and close the wound field.
Cell proliferation and migration rates can be determined using manual fixing and microscopic imaging. A fixing solution is provided for stopping cells at specific time points. Cell stain and DAPI stain are also provided for viewing results with light and fluorescence microscopy.
Product promise
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5 product images
Cellular Activity - Wound Healing Assay (ab242285)
Wound healing.
Schematic Diagram - Wound Healing Assay (ab242285)
Assay Principle.
Schematic Diagram - Wound Healing Assay (ab242285)
Wound Healing Assay Plate Assembly.
Cellular Activity - Wound Healing Assay (ab242285)
Example of Wound Field Surface Area.
Cellular Activity - Wound Healing Assay (ab242285)
Percent Closure of MEF/STO Cells.
STO cells were tested using ab242285. Cells were cultured 24 hours until a monolayer formed at which time the inserts were removed to begin the wound healing assay. Cells were monitored under phase contrast (not shown), DAPI labeling, and cell staining for determining percent closure (0, 50, 75, and 100%).
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