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AB242285

Wound Healing Assay

5

(1 Review)

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(15 Publications)

Wound Healing Assay Kit ab242285 is suitable for measuring cell proliferation, cell migration and wound closure in adherent cells. It contains 2 x 24-well plates, each containing 12 proprietary treated plastic inserts for clear staining. Staining solution, fixative and dye also included.

- More consistent results compared to other types of wound healing assays
- Inserts leave no residues that could impede proliferation or migration of cells
- Compatible with light and fluorescence microscopy
5 Images
Functional Studies (Act) - Wound Healing Assay (AB242285)
  • FuncS (Act)

Supplier Data

Functional Studies (Act) - Wound Healing Assay (AB242285)

Wound healing.

Functional Studies (Act) - Wound Healing Assay (AB242285)
  • FuncS (Act)

Supplier Data

Functional Studies (Act) - Wound Healing Assay (AB242285)

Example of Wound Field Surface Area.

Functional Studies (Act) - Wound Healing Assay (AB242285)
  • FuncS (Act)

Supplier Data

Functional Studies (Act) - Wound Healing Assay (AB242285)

Percent Closure of MEF/STO Cells.

STO cells were tested using ab242285. Cells were cultured 24 hours until a monolayer formed at which time the inserts were removed to begin the wound healing assay. Cells were monitored under phase contrast (not shown), DAPI labeling, and cell staining for determining percent closure (0, 50, 75, and 100%).

Schematic Diagram - Wound Healing Assay (AB242285)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Wound Healing Assay (AB242285)

Assay Principle.

Schematic Diagram - Wound Healing Assay (AB242285)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Wound Healing Assay (AB242285)

Wound Healing Assay Plate Assembly.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Adherent cells

Assay type

Cell-based

Assay Platform

Microscope

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Product details

Wound Healing Assay Kit (ab242285) contains 2 x 24-well plates each containing 12 proprietary treated plastic inserts. The inserts create a wound field with a defined gap of 0.9 mm for measuring the migratory and proliferation rates of cells. Migratory cells are able to extend protrusions and ultimately invade and close the wound field.

Cell proliferation and migration rates can be determined using manual fixing and microscopic imaging. A fixing solution is provided for stopping cells at specific time points. Cell stain and DAPI stain are also provided for viewing results with light and fluorescence microscopy.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "24Test": { "sellingSize": "24 Test", "publicAssetCode":"ab242285-24Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"24-Well Wound Healing Assay Plate", "number":"AB242285-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Fixation Solution", "number":"AB242285-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Stain Solution", "number":"AB242285-CMP02", "productcode":"" }, { "size":"1 x 30 µL", "name":"DAPI Fluorescence Stain (1000X)", "number":"AB242285-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:458 PubMed40253339

2025

Peptides based on the interface of hnRNPA2B1-transthyretin complex repress retinal angiogenesis in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yixiu Chen,Lu Ye,Shujing Cui,Jun Shao,Yu Xin

ACS omega 9:31776-31788 PubMed39072128

2024

Managing Oxidative Stress Using Vitamin C to Improve Biocompatibility of Polycaprolactone for Bone Regeneration .

Applications

Unspecified application

Species

Unspecified reactive species

Elaf Akram Abdulhameed,K G Aghila Rani,Fatima Mousa AlGhalban,Ensanya A Abou Neel,Nadia Khalifa,Khalil Abdelrazek Khalil,Marzuki Omar,Ab Rani Samsudin

Oncology 102:868-879 PubMed38442705

2024

Nesprin1 Deficiency Is Associated with Poor Prognosis of Renal Cell Carcinoma and Resistance to Sunitinib Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Fukushima,Kohei Kobatake,Kento Miura,Kenshiro Takemoto,Ryoken Yamanaka,Ryo Tasaka,Yuki Kohada,Shunsuke Miyamoto,Yohei Sekino,Hiroyuki Kitano,Keisuke Goto,Kenichiro Ikeda,Akihiro Goriki,Keisuke Hieda,Osamu Kaminuma,Nobuyuki Hinata

Pharmaceutics 16: PubMed38399220

2024

Innovative Multilayer Electrospun Patches for the Slow Release of Natural Oily Extracts as Dressings to Boost Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Noemi Fiaschini,Fiorella Carnevali,Stephen Andrew Van der Esch,Roberta Vitali,Mariateresa Mancuso,Maria Sulli,Gianfranco Diretto,Anna Negroni,Antonio Rinaldi

Antioxidants (Basel, Switzerland) 13: PubMed38397757

2024

Selenium Compounds Affect Differently the Cytoplasmic Thiol/Disulfide State in Dermic Fibroblasts and Improve Cell Migration by Interacting with the Extracellular Matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Kreindl,Sandra A Soto-Alarcón,Miltha Hidalgo,Ana L Riveros,Carolina Añazco,Rodrigo Pulgar,Omar Porras

Molecular reproduction and development 90:758-770 PubMed37548351

2023

lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in pre-eclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyun Wan,Jia Huang,Wenting Liu,Xiaoyan Su,Bei Zhao,Xianggang Wang,Lu Zhao

BioFactors (Oxford, England) 49:1158-1173 PubMed37338025

2023

SUMO2/3 promotes the progression and oxaliplatin resistance of colorectal cancer through facilitating the SUMOylation at Ku80-K307.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Feng,Jinsong He,Min Yuan,Qing Chen,Xi Zeng,Qilin Zhou,Jian Wu,Bin Han

Diabetic medicine : a journal of the British Diabetic Association 40:e15125 PubMed37186403

2023

The interface targeting hnRNPA2B1 regulates the repression of transthyretin against human retinal microvascular endothelial cells in high-glucose environment.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Tian,Lu Ye,Jun Shao,Yu Xin

Scientific reports 12:17856 PubMed36284122

2022

Co-alterations of circadian clock gene transcripts in human placenta in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Guoli Zhou,Emily Winn,Duong Nguyen,Eric P Kasten,Margaret G Petroff,Hanne M Hoffmann

Science advances 8:eabq8297 PubMed36269833

2022

loss transforms -deficient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lorea Valcarcel-Jimenez,Connor Rogerson,Cissy Yong,Christina Schmidt,Ming Yang,Monica Cremades-Rodelgo,Victoria Harle,Victoria Offord,Kim Wong,Ariane Mora,Alyson Speed,Veronica Caraffini,Maxine Gia Binh Tran,Eamonn R Maher,Grant D Stewart,Sakari Vanharanta,David J Adams,Christian Frezza
View all publications
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