WST-1 Assay Reagent - Cell Proliferation (ready to use)

Product details

WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells.

The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. The larger the number of viable cells, the higher the activity of the mitochondrial dehydrogenases, and in turn the greater the amount of formazan dye formed.

The WST-1 assay protocol is very simple:
- add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
- shake the plate to mix the contents
- analyze the amount of formazan dye produced by measuring the absorbance at 440 nm

WST-1 assay kit ab65473 is the full kit version of this reagent, which includes the Electrocoupling Reagent, in addition to the WST-1 Assay Reagent.

WST-1 assays are non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.