WST-8 Assay Kit ab65475 is an alternative version of ab65473, our most popular WST-1 Assay Kit.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of molecular sciences 24:2023Advances in the Properties of Incomptine A: Cytotoxic Activity and Downregulation of Hexokinase II in Breast Cancer Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngel Giovanni Arietta-García,Fernando Calzada,Israel Ramírez-Sánchez,Elihú Bautista,Normand García-Hernandez,Rosa María Ordoñez-RazoPubMed 37569780
- Life (Basel, Switzerland) 13:2023Sphingosine-1-phosphate Treatment Improves Cryopreservation Efficiency in Human Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeong-Ju Oh,Chan-Hee Jo,Tae-Seok Kim,Chae-Yeon Hong,Sung-Lim Lee,Young-Hoon Kang,Gyu-Jin RhoPubMed 37374070
- Molecules (Basel, Switzerland) 27:2022Understanding the Antilymphoma Activity of Donn and Its Acyclic Terpenoids: In Vivo, In Vitro, and In Silico Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJesica Ramírez-Santos,Fernando Calzada,Jessica Elena Mendieta-Wejebe,Rosa María Ordoñez-Razo,Rubria Marlen Martinez-Casares,Miguel ValdesPubMed 36296714
- iScience 25:1051352022Deficiency of peroxisomal NUDT7 stimulates lipogenesis in hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinsoo Song,In-Jeoung Baek,Sujeong Park,Jinjoo Oh,Deokha Kim,Kyung Song,Mi Kyung Kim,Hye Won Lee,Byoung Kuk Jang,Eun-Jung JinPubMed 36185359
- International journal of biological sciences 18:4203-42182022TOP1 inhibition induces bifurcated JNK/MYC signaling that dictates cancer cell sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQizhi Liu,Stacey Chung,Michael M Murata,Bingchen Han,Bowen Gao,Maoqi Zhang,Tian-Yu Lee,Evgeny Chirshev,Juli Unternaehrer,Hisashi Tanaka,Armando E Giuliano,Yukun Cui,Xiaojiang CuiPubMed 35844787
- Experimental and therapeutic medicine 23:3892022Immunostimulatory activity of soybean hull polysaccharide on macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxue Wang,Chenye Fu,Mingcong Zhang,Yuxian Zhang,Liang CaoPubMed 35495602
- Molecular cell 82:1107-1122.e72022Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Biancon,Poorval Joshi,Joshua T Zimmer,Torben Hunck,Yimeng Gao,Mark D Lessard,Edward Courchaine,Andrew E S Barentine,Martin Machyna,Valentina Botti,Ashley Qin,Rana Gbyli,Amisha Patel,Yuanbin Song,Lea Kiefer,Gabriella Viero,Nils Neuenkirchen,Haifan Lin,Joerg Bewersdorf,Matthew D Simon,Karla M Neugebauer,Toma Tebaldi,Stephanie HalenePubMed 35303483
- Molecules (Basel, Switzerland) 26:2021Antitumor Potential of Linn. An Edible and Medicinal Plant in Mexico: In Vitro, In Vivo, and Toxicological Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVerenice Merlín-Lucas,Rosa María Ordoñez-Razo,Fernando Calzada,Aida Solís,Normand García-Hernández,Elizabeth Barbosa,Miguel ValdésPubMed 34946755
- American journal of physiology. Cell physiology 320:C943-C9552021Inhibition of P110α and P110δ catalytic subunits of PI3 kinase reverses impaired arterial healing after injury in hypercholesterolemic male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPinaki Chaudhuri,Andrew H Smith,Linda M Graham,Michael A RosenbaumPubMed 33689479
- American journal of physiology. Cell physiology 320:C731-C7412021P110α and P110δ catalytic subunits of PI3 kinase regulate lysophosphatidylcholine-induced TRPC6 externalization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPinaki Chaudhuri,Andrew H Smith,Priya Putta,Linda M Graham,Michael A RosenbaumPubMed 33625929
Key facts
- Detection method
Colorimetric
- Sample types
Suspension cells, Adherent cells
- Assay type
Quantitative
- Assay time
4h
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WST-8 Assay Kit ab65475 is an alternative version of ab65473, our most popular WST-1 Assay Kit.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric
- Sample types
Suspension cells, Adherent cells
- Assay type
Quantitative
- Assay time
4h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
WST-8 Assay Kit ab65475 (previously called WST-1 Assay Kit) is an alternative version of WST-1 Assay Kit (Cell Proliferation) ab65473, our most popular WST-1 Assay Kit (which is also supplied as a ready-to-use reagent WST-1 Assay Reagent - Cell Proliferation (ready to use) ab155902). This kit uses a different WST-8 analog, and also contains a stop solution.
The WST-8 assay provides by far the easiest and most sensitive means for performing a quantitative cell proliferation assay, cell viability assay, or cytotoxicity assay in mammalian cells.
The WST-8 assay protocol is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by microplate reader by measuring the absorbance of the dye solution at 440 nm.
The WST-8 assay protocol is very simple:
- add the WST-8 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
- shake the plate to mix the contents
- analyze the amount of formazan dye produced by measuring the absorbance at 440 nm
This kit contains an optional stop solution to stop the production of formazan dye.
This product is manufactured by BioVision, an Abcam company and was previously called K302 Quick Cell Proliferation colorimetric Assay Kit Plus. K302-2500 is the same size as the 2500 test size of ab65475.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Cellular Activity - WST-1 Assay Kit (Cell Proliferation) (ab65475)
Mitochondrial dehydrogenases checked in various cell concentrations at different incubation times (2h WST for each condition)
Cellular Activity - WST-1 Assay Kit (Cell Proliferation) (ab65475)
HL60 cell proliferation measured after 24h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
Cellular Activity - WST-1 Assay Kit (Cell Proliferation) (ab65475)
HL60 cell proliferation measured after 48h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
Cellular Activity - WST-1 Assay Kit (Cell Proliferation) (ab65475)
HL60 cell proliferation measured after 72h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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