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AB155900

Xanthine/Hypoxanthine Assay Kit

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(9 Publications)

Xanthine/Hypoxanthine Assay kit (ab155900) is a product where Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (570 nm) or fluorometrically (Ex/Em = 535/587 nm).

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Xanthine/Hypoxanthine Assay Kit (AB155900)
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Lab

Functional Studies - Xanthine/Hypoxanthine Assay Kit (AB155900)

Xanthine & Hypoxanthine measured in mouse tissue lysates by fluorimetric method showing quantity (nmol) per mg of sample tested.

Functional Studies - Xanthine/Hypoxanthine Assay Kit (AB155900)
  • FuncS

Lab

Functional Studies - Xanthine/Hypoxanthine Assay Kit (AB155900)

Xanthine & Hypoxanthine measured in mouse tissue lysates by colorimetric method showing quantity (mmol) per mg of sample tested.

Functional Studies - Xanthine/Hypoxanthine Assay Kit (AB155900)
  • FuncS

Lab

Functional Studies - Xanthine/Hypoxanthine Assay Kit (AB155900)

Xanthine & Hypoxanthine measured in biological fluids by fluorimetric method showing concentrations (micromolar).

Schematic Diagram - Xanthine/Hypoxanthine Assay Kit (AB155900)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Xanthine/Hypoxanthine Assay Kit (AB155900)

Representative image of Xanthine/Hypoxanthine Assay Kit ab155900

Components shown from left to right :

- OxiRed Probe

- Xanthine Enzyme Mix

- Xanthine Standard

- Developer Solution V

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Tissue, Suspension cells, Tissue Homogenate, Cell culture supernatant, Milk, Serum, Other biological fluids, Adherent cells

Assay type

Enzyme activity

Sensitivity

= 0.4 µM

Assay time

1h

Assay Platform

Microplate reader

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Product details

Xanthine/Hypoxanthine Assay kit (ab155900) is a product where Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (570 nm) or fluorometrically (Ex/Em = 535/587 nm). Xanthine/Hypoxanthine Assay kit is rapid, simple and sensitive. This high-throughput suitable assay kit can detect Xanthine levels as low as 0.4 uM in various biological samples.

Other Notes
This product was previously called K685 Biovision Xanthine/Hypoxanthine Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Xanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for diagnostic and therapeutic monitoring.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab155900-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Xanthine Standard", "number":"AB155900-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Xanthine Enzyme Mix", "number":"AB155900-CMP04", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB155900-CMP01", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB155900-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB155900-CMP02", "productcode":"AB136652" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Xanthine and hypoxanthine are purine derivatives and key intermediates in the purine degradation pathway. They play a critical role in catabolism by converting hypoxanthine to xanthine which subsequently transforms into uric acid by xanthine oxidase. These compounds are expressed ubiquitously in tissues where purine metabolism is active. Xanthine's molecular mass is 152.11 g/mol while hypoxanthine has a molecular mass of 136.11 g/mol. Hypoxanthine is also considered a substrate for the salvage pathways in nucleotide synthesis providing an alternate route to synthesize important nucleotides.
Biological function summary

Xanthine and hypoxanthine function as central components in the cellular degradation of purines participating actively in the turnover of nucleic acids. They are not part of any specific protein complex but interact with enzymes like xanthine oxidase which facilitates the oxidation processes. These molecules play an essential role in ensuring the balanced recycling of purine nucleotides which are necessary for cellular energy transactions and genetic material synthesis.

Pathways

These molecules are integral to purine metabolism pathways and are linked with the degradation and salvage pathways. Xanthine and hypoxanthine are related through their connection with enzymes such as xanthine oxidase and hypoxanthine-guanine phosphoribosyltransferase which regulate the critical steps of converting them into uric acid and nucleotides. These interactions position them as influential players in maintaining cellular homeostasis and energy balance by recycling purines effectively.

Xanthine and hypoxanthine have significant links to conditions such as gout and Lesch-Nyhan syndrome. Gout arises from high levels of uric acid formed from xanthine leading to painful joint inflammation. Lesch-Nyhan syndrome connects with hypoxanthine due to a deficiency in hypoxanthine-guanine phosphoribosyltransferase causing an aberrant nucleotide metabolism. These relationships underline the importance of maintaining the correct functional levels of xanthine and hypoxanthine to prevent such metabolic disorders.
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Product protocols

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Target data

See full target information Xanthine
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:139 PubMed38485739

2024

Purine salvage-associated metabolites as biomarkers for early diagnosis of esophageal squamous cell carcinoma: a diagnostic model-based study.

Applications

Unspecified application

Species

Unspecified reactive species

Yawen Sun,Wenjuan Liu,Mu Su,Tao Zhang,Xia Li,Wenbin Liu,Yuping Cai,Deli Zhao,Ming Yang,Zhengjiang Zhu,Jialin Wang,Jinming Yu

Scientific reports 14:253 PubMed38167685

2024

H-NMR metabolomics analysis identifies hypoxanthine as a novel metastasis-associated metabolite in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sarra B Shakartalla,Naglaa S Ashmawy,Mohammad H Semreen,Bahgat Fayed,Zainab M Al Shareef,Manju N Jayakumar,Saleh Ibrahim,Mohamed Rahmani,Rania Hamdy,Sameh S M Soliman

Cardiovascular research 118:2847-2858 PubMed34668514

2021

Ogfod1 deletion increases cardiac beta-alanine levels and protects mice against ischaemia- reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Harris,Junhui Sun,Karen Keeran,Angel Aponte,Komudi Singh,Danielle Springer,Marjan Gucek,Mehdi Pirooznia,Matthew E Cockman,Elizabeth Murphy,Leslie M Kennedy

Kidney360 2:1793-1806 PubMed35372998

2021

Deletion of Induces Xanthinuria with Obstructive Nephropathy and Major Metabolic Disorders in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Delphine Sedda,Claire Mackowiak,Julie Pailloux,Elodie Culerier,Ana Dudas,Pauline Rontani,Nicolas Erard,Antoine Lefevre,Sylvie Mavel,Patrick Emond,Frederic Foucher,Marc Le Bert,Valerie F J Quesniaux,Michael J Mihatsch,Bernhard Ryffel,Madeleine Erard-Garcia

Mikrochimica acta 186:749 PubMed31696297

2019

Recent progress in nanomaterial-based electrochemical and optical sensors for hypoxanthine and xanthine. A review.

Applications

Unspecified application

Species

Unspecified reactive species

Muamer Dervisevic,Esma Dervisevic,Mehmet Şenel

Journal of cellular and molecular medicine 23:8025-8034 PubMed31602751

2019

Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Florent Lemaire,Séverine Sigrist,Eric Delpy,Julien Cherfan,Claude Peronet,Franck Zal,Karim Bouzakri,Michel Pinget,Elisa Maillard

PloS one 12:e0186656 PubMed29049352

2017

Exposure to intrauterine inflammation alters metabolomic profiles in the amniotic fluid, fetal and neonatal brain in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Amy G Brown,Natalia M Tulina,Guillermo O Barila,Michael S Hester,Michal A Elovitz

Journal of neuroscience research 94:1094-107 PubMed27638595

2016

Metabolic profiling reveals biochemical pathways and potential biomarkers associated with the pathogenesis of Krabbe disease.

Applications

Unspecified application

Species

Unspecified reactive species

Nadav I Weinstock,Lawrence Wrabetz,M Laura Feltri,Daesung Shin

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 35:2412-21 PubMed25967871

2015

Using Next-Generation Sequencing to Identify a Mutation in Human MCSU that is Responsible for Type II Xanthinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Yunan Zhou,Xueguang Zhang,Rui Ding,Zuoxiang Li,Quan Hong,Yan Wang,Wei Zheng,Xiaodong Geng,Meng Fan,Guangyan Cai,Xiangmei Chen,Di Wu
View all publications
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