AB102522

Xanthine Oxidase Activity Assay Kit

Name: Xanthine Oxidase Activity Assay Kit (ab102522) | Abcam
Brand: Abcam Limited
SKU: AB102522
Price: 500 USD
Availability: InStock

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(23 Publications)

Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

XDHA, XDH, Xanthine dehydrogenase/oxidase

2 Images

Functional Studies - Xanthine Oxidase Activity Assay Kit (AB102522)

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Functional Studies - Xanthine Oxidase Activity Assay Kit (AB102522)

Representative Standard Curve using ab102522.

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Supplier Data

Functional Studies - Xanthine Oxidase Activity Assay Kit (AB102522)

Xanthine Oxidase Positive Control.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Milk, Serum, Other biological fluids

Assay type

Enzyme activity

Sensitivity

> 10 mU/mL

Range

10 - 1000 mU/mL

Assay time

40m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.

In the xanthine oxidase assay protocol, xanthine oxidase oxidizes xanthine to hydrogen peroxide (H₂O₂) which reacts stoichiometrically with a probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured.

The kit detects 1-100 mU xanthine oxidase in 100 μl reaction volume.

Xanthine oxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader immediately and after 10-20 min

This product is manufactured by BioVision, an Abcam company and was previously called K710 Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit. K710-100 is the same size as the 100 test size of ab102522.

Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.

Biological function summary

Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.

Pathways

The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.

Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.

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Product protocols

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Target data

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

See full target information XDH

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal physiology and animal nutrition 108:1734-1746 PubMed38956882

2024

Effect of dietary supplementation of macadamia oil on the growth, immune function, physio-biochemical components and thyroid activity of growing rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima A Jaber,Samia Nasr,Hanan M A Al-Sayed,Aljohara M Al-Otaibi,Hanan S Mohamed,Haggag M Hamdy,Sameh A Abdelnour,Walid Fouad

Antioxidants (Basel, Switzerland) 12: PubMed37891894

2023

In Vitro Antioxidant and In Vivo Antigenotoxic Features of a Series of 61 Essential Oils and Quantitative Composition-Activity Relationships Modeled through Machine Learning Algorithms.

Applications

Unspecified application

Species

Unspecified reactive species

Milan Mladenović,Roberta Astolfi,Nevena Tomašević,Sanja Matić,Mijat Božović,Filippo Sapienza,Rino Ragno

Heliyon 9:e18069 PubMed37483701

2023

Evaluation of anti-hyperuricemic effects of Miq. (Keladi Candik) extracts in potassium oxonate induced rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Ferid Abdulhafiz,Arifullah Mohammed,Mohd Farhan Hanif Reduan,Zulhazman Hamzah,Zulhisyam Abdul Kari,Guillermo Téllez-Isaías

Cancer cell 41:356-372.e10 PubMed36706760

2023

Neutrophil-activating therapy for the treatment of cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ian L Linde,Tyler R Prestwood,Jingtao Qiu,Genay Pilarowski,Miles H Linde,Xiangyue Zhang,Lei Shen,Nathan E Reticker-Flynn,David Kung-Chun Chiu,Lauren Y Sheu,Simon Van Deursen,Lorna L Tolentino,Wen-Chao Song,Edgar G Engleman

BMC complementary medicine and therapies 22:278 PubMed36271349

2022

Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxing Shui,Zheyi Cai,Feng Wang,Ting Chen,Xueyuan Huang,Yun Cai,Xiuhua Mi

Scientific reports 12:14056 PubMed35982129

2022

Effects of Thai native chicken breast meat consumption on serum uric acid level, biochemical parameters, and antioxidant activities in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Prapassorn Potue,Petcharat Chiangsaen,Putcharawipa Maneesai,Juthamas Khamseekaew,Poungrat Pakdeechote,Vibuntita Chankitisakul,Wuttigrai Boonkum,Natthaya Duanghaklang,Monchai Duangjinda

Antioxidants (Basel, Switzerland) 10: PubMed34829529

2021

Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Gonzaléz-Candia,Pamela V Arias,Simón A Aguilar,Esteban G Figueroa,Roberto V Reyes,Germán Ebensperger,Aníbal J Llanos,Emilio A Herrera

Clinical and translational science 14:1431-1443 PubMed33931953

2021

Oxypurinol pharmacokinetics and pharmacodynamics in healthy volunteers: Influence of BCRP Q141K polymorphism and patient characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Vora,Deanna J Brackman,Ling Zou,Maria Garcia-Cremades,Marina Sirota,Radojka M Savic,Kathleen M Giacomini

Cancer discovery 11:500-519 PubMed33028621

2020

The Hepatic Microenvironment Uniquely Protects Leukemia Cells through Induction of Growth and Survival Pathways Mediated by LIPG.

Applications

Unspecified application

Species

Unspecified reactive species

Haobin Ye,Mohammad Minhajuddin,Anna Krug,Shanshan Pei,Chih-Hsing Chou,Rachel Culp-Hill,Jessica Ponder,Erik De Bloois,Björn Schniedewind,Maria L Amaya,Anagha Inguva,Brett M Stevens,Daniel A Pollyea,Uwe Christians,H Leighton Grimes,Angelo D'Alessandro,Craig T Jordan

Aging 11:12177-12201 PubMed31841118

2019

Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Zilu,Huang Qian,Wu Haibin,Ge Chenxu,Lou Deshuai,Li Qiang,Hu Linfeng,Tan Jun,Xu Minxuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Xanthine Oxidase Activity Assay Kit

Functional Studies - Xanthine Oxidase Activity Assay Kit (AB102522)

Functional Studies - Xanthine Oxidase Activity Assay Kit (AB102522)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise