Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Antioxidants (Basel, Switzerland) 12:2023In Vitro Antioxidant and In Vivo Antigenotoxic Features of a Series of 61 Essential Oils and Quantitative Composition-Activity Relationships Modeled through Machine Learning Algorithms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilan Mladenović,Roberta Astolfi,Nevena Tomašević,Sanja Matić,Mijat Božović,Filippo Sapienza,Rino RagnoPubMed 37891894
- Heliyon 9:e180692023Evaluation of anti-hyperuricemic effects of Miq. (Keladi Candik) extracts in potassium oxonate induced rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFerid Abdulhafiz,Arifullah Mohammed,Mohd Farhan Hanif Reduan,Zulhazman Hamzah,Zulhisyam Abdul Kari,Guillermo Téllez-IsaíasPubMed 37483701
- Cancer cell 41:356-372.e102023Neutrophil-activating therapy for the treatment of cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan L Linde,Tyler R Prestwood,Jingtao Qiu,Genay Pilarowski,Miles H Linde,Xiangyue Zhang,Lei Shen,Nathan E Reticker-Flynn,David Kung-Chun Chiu,Lauren Y Sheu,Simon Van Deursen,Lorna L Tolentino,Wen-Chao Song,Edgar G EnglemanPubMed 36706760
- BMC complementary medicine and therapies 22:2782022Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangxing Shui,Zheyi Cai,Feng Wang,Ting Chen,Xueyuan Huang,Yun Cai,Xiuhua MiPubMed 36271349
- Scientific reports 12:140562022Effects of Thai native chicken breast meat consumption on serum uric acid level, biochemical parameters, and antioxidant activities in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrapassorn Potue,Petcharat Chiangsaen,Putcharawipa Maneesai,Juthamas Khamseekaew,Poungrat Pakdeechote,Vibuntita Chankitisakul,Wuttigrai Boonkum,Natthaya Duanghaklang,Monchai DuangjindaPubMed 35982129
- Antioxidants (Basel, Switzerland) 10:2021Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlejandro Gonzaléz-Candia,Pamela V Arias,Simón A Aguilar,Esteban G Figueroa,Roberto V Reyes,Germán Ebensperger,Aníbal J Llanos,Emilio A HerreraPubMed 34829529
- Clinical and translational science 14:1431-14432021Oxypurinol pharmacokinetics and pharmacodynamics in healthy volunteers: Influence of BCRP Q141K polymorphism and patient characteristics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Vora,Deanna J Brackman,Ling Zou,Maria Garcia-Cremades,Marina Sirota,Radojka M Savic,Kathleen M GiacominiPubMed 33931953
- Cancer discovery 11:500-5192020The Hepatic Microenvironment Uniquely Protects Leukemia Cells through Induction of Growth and Survival Pathways Mediated by LIPG.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaobin Ye,Mohammad Minhajuddin,Anna Krug,Shanshan Pei,Chih-Hsing Chou,Rachel Culp-Hill,Jessica Ponder,Erik De Bloois,Björn Schniedewind,Maria L Amaya,Anagha Inguva,Brett M Stevens,Daniel A Pollyea,Uwe Christians,H Leighton Grimes,Angelo D'Alessandro,Craig T JordanPubMed 33028621
- Aging 11:12177-122012019Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi Zilu,Huang Qian,Wu Haibin,Ge Chenxu,Lou Deshuai,Li Qiang,Hu Linfeng,Tan Jun,Xu MinxuanPubMed 31841118
- Journal of pineal research 68:e126132019Melatonin long-lasting beneficial effects on pulmonary vascular reactivity and redox balance in chronic hypoxic ovine neonates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlejandro Gonzaléz-Candia,Alejandro A Candia,Esteban G Figueroa,Elisenda Feixes,Cristopher Gonzalez-Candia,Simón A Aguilar,Germán Ebensperger,Roberto V Reyes,Aníbal J Llanos,Emilio A HerreraPubMed 31583753
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Milk, Serum, Other biological fluids
- Assay type
Enzyme activity
- Range
10 - 1000 mU/mL
- Assay time
40m
- Sensitivity
> 10 mU/mL
Target data
Function
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
Alternative names
Xanthine dehydrogenase/oxidase, XDH, XDHA
What's included?
Recommended products
Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Xanthine dehydrogenase/oxidase, XDH, XDHA
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Milk, Serum, Other biological fluids
- Assay type
Enzyme activity
- Range
10 - 1000 mU/mL
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 10 mU/mL
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.
In the xanthine oxidase assay protocol, xanthine oxidase oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with a probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured.
The kit detects 1-100 mU xanthine oxidase in 100 μl reaction volume.
Xanthine oxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader immediately and after 10-20 min
This product is manufactured by BioVision, an Abcam company and was previously called K710 Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit. K710-100 is the same size as the 100 test size of ab102522.
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.
- Biological function summary
Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.
- Pathways
The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.
- Associated diseases and disorders
Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.
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2 product images
Functional Studies - Xanthine Oxidase Activity Assay Kit (ab102522)
Representative Standard Curve using ab102522.
Functional Studies - Xanthine Oxidase Activity Assay Kit (ab102522)
Xanthine Oxidase Positive Control.
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