XTT Assay Kit ab232856 provides an easy to use tool for use with cell cultures to study changes in the number of cells and their metabolic activity. Readout on any colorimetric (450 nm) plate reader.
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- Reproductive sciences (Thousand Oaks, Calif.) 31:2293-23082024Does the miR-105-1-Kisspeptin Axis Promote Ovarian Cell Functions?Applications:Unspecified applicationReactive species:Unspecified reactive speciesZuzana Fabová,Barbora Loncová,Abdel Halim Harrath,Alexander V SirotkinPubMed 38632222
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- Journal of applied toxicology : JAT 42:570-5872021Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Barbasz,Agnieszka Czyżowska,Natalia Piergies,Magdalena OćwiejaPubMed 34558088
- Journal of applied toxicology : JAT 42:334-3412021The cell membrane as the barrier in the defense against nanoxenobiotics: Zinc oxide nanoparticles interactions with native and model membrane of melanoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Czyżowska,Anna Barbasz,Elżbieta Rudolphi-Szydło,Barbara DybaPubMed 34235764
- Communications biology 4:7012021Placental secretome characterization identifies candidates for pregnancy complications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTina Napso,Xiaohui Zhao,Marta Ibañez Lligoña,Ionel Sandovici,Richard G Kay,Amy L George,Fiona M Gribble,Frank Reimann,Claire L Meek,Russell S Hamilton,Amanda N Sferruzzi-PerriPubMed 34103657
- Molecules (Basel, Switzerland) 26:2021Effects of 3FTx Protein Fraction from Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Dyba,Elżbieta Rudolphi-Szydło,Anna Barbasz,Agnieszka Czyżowska,Konrad Kamil Hus,Vladimír Petrilla,Monika Petrillová,Jaroslav Legáth,Aleksandra BocianPubMed 33918763
- Scientific reports 11:9732021Combined effect of arginine and fluoride on the growth of Lactobacillus rhamnosus GG.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed Nadeem Bijle et. al.PubMed 33441658
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
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XTT Assay Kit ab232856 provides an easy to use tool for use with cell cultures to study changes in the number of cells and their metabolic activity. Readout on any colorimetric (450 nm) plate reader.
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
XTT Assay Kit ab232856 provides an easy to use tool for use with cell cultures to study changes in the number of cells and their metabolic activity. This provides insights into cell viability, cell proliferation, and cytotoxicity.
XTT assay principle
The XTT assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator. Reduction of XTT during the assay produces a water-soluble orange-colored formazan product which dissolves directly into the culture medium, eliminating the need for an additional solubilization step (as is required in MTT assay kit MTT Assay Kit (Cell Proliferation) ab211091, but not in MTS assay kit MTS Assay Kit (Cell Proliferation) (Colorimetric) ab197010). The formazan product can be detected using an absorbance-based microplate reader (450 nm).
As the reagents used in the XTT and their products are non-toxic and the assay does not involve cell lysis, the XTT assay can be used to measure multiple timepoints or for continual measurement.
XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) is a tetrazolium-based compound like the reagents used in the MTT assay and the MTS assay.
XTT assay protocol summary
- add XTT mixture to cell culture wells
- mix for 1 min
- incubate for 2-4 hrs
- mix for 1 min
- analyze with a plate reader
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Functional Studies - XTT Assay Kit (ab232856)
A typical cell titration experiment using HL-60 target cells and XTT assay kit ab232856.
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