Zinc Assay Kit ab102507 is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- Protoplasma 261:487-4962023Elevated luteinizing hormone receptor signaling or selenium treatment leads to comparable changes in adrenal cortex histology and androgen-AR/ZIP9 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaroslaw Wieczorek,Piotr Pawlicki,Marta Zarzycka,Laura Pardyak,Piotr Niedbala,Michal Duliban,Begum Yurdakok-Dikmen,Malgorzata Kotula-BalakPubMed 38052957
- Cancer diagnosis & prognosis 2:184-1932022Clinical Significance of Serum Zinc Levels on the Development of Sarcopenia in Cirrhotic Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKoji Murata,Tadashi Namisaki,Yuki Fujimoto,Soichi Takeda,Masahide Enomoto,Hiroaki Takaya,Yuki Tsuji,Akihiko Shibamoto,Junya Suzuki,Takahiro Kubo,Satoshi Iwai,Fumimasa Tomooka,Misako Tanaka,Miki Kaneko,Shohei Asada,Aritoshi Koizumi,Nobuyuki Yorioka,Takuya Matsuda,Takahiro Ozutsumi,Koji Ishida,Hiroyuki Ogawa,Hirotetsu Takagi,Yukihisa Fujinaga,Masanori Furukawa,Yasuhiko Sawada,Norihisa Nishimura,Koh Kitagawa,Shinya Sato,Kosuke Kaji,Takashi Inoue,Kiyoshi Asada,Hideto Kawaratani,Kei Moriya,Takemi Akahane,Akira Mitoro,Hitoshi YoshijiPubMed 35399181
- Frontiers in immunology 12:7855992022Hypozincemia in COVID-19 Patients Correlates With Stronger Antibody Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenye Xu,Yingzhi Liu,Xuan Zou,Huanle Luo,Weihua Wu,Junjie Xia,Matthew T V Chan,Shisong Fang,Yuelong Shu,William K K Wu,Lin ZhangPubMed 35058926
- Saudi journal of biological sciences 29:2958-29682022Melatonin attenuates cerebral hypoperfusion-induced hippocampal damage and memory deficits in rats by suppressing TRPM7 channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHussain Al Dera,Mohammed Alassiri,Reem Al Kahtani,Samy M Eleawa,Mohammad K AlMulla,Abdulhakeem AlamriPubMed 35531206
- Cell reports 37:1101322021Resolving the immune landscape of human prostate at a single-cell level in health and cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZewen Kelvin Tuong,Kevin W Loudon,Brendan Berry,Nathan Richoz,Julia Jones,Xiao Tan,Quan Nguyen,Anne George,Satoshi Hori,Sarah Field,Andy G Lynch,Katarzyna Kania,Paul Coupland,Anne Babbage,Richard Grenfell,Tristan Barrett,Anne Y Warren,Vincent Gnanapragasam,Charlie Massie,Menna R ClatworthyPubMed 34936871
- Metabolic brain disease 37:299-3102021Impaired ion homeostasis as a possible associate factor in mucopolysaccharidosis pathogenesis: transcriptomic, cellular and animal studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLidia Gaffke,Zuzanna Szczudło,Magdalena Podlacha,Zuzanna Cyske,Estera Rintz,Jagoda Mantej,Karolina Krzelowska,Grzegorz Węgrzyn,Karolina PierzynowskaPubMed 34928474
- Journal of cellular and molecular medicine 25:8432-84412021SLC39A5 dysfunction impairs extracellular matrix synthesis in high myopia pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Dong,Qi Tian,Tengfei Zhu,Kangli Wang,Ganting Lei,Yanling Liu,Haofeng Xiong,Lu Shen,Meng Wang,Rongjuan Zhao,Huidan Wu,Bin Li,Qiumeng Zhang,Yujun Yao,Hui Guo,Kun Xia,Lu Xia,Zhengmao HuPubMed 34302427
- Acta neuropathologica 141:725-7542021Age-associated insolubility of parkin in human midbrain is linked to redox balance and sequestration of reactive dopamine metabolites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline M Tokarew,Daniel N El-Kodsi,Nathalie A Lengacher,Travis K Fehr,Angela P Nguyen,Bojan Shutinoski,Brian O'Nuallain,Ming Jin,Jasmine M Khan,Andy C H Ng,Juan Li,Qiubo Jiang,Mei Zhang,Liqun Wang,Rajib Sengupta,Kathryn R Barber,An Tran,Doo Soon Im,Steve Callaghan,David S Park,Stephanie Zandee,Xiajun Dong,Clemens R Scherzer,Alexandre Prat,Eve C Tsai,Masashi Takanashi,Nobutaka Hattori,Jennifer A Chan,Luigi Zecca,Andrew B West,Arne Holmgren,Lawrence Puente,Gary S Shaw,Gergely Toth,John M Woulfe,Peggy Taylor,Julianna J Tomlinson,Michael G SchlossmacherPubMed 33694021
- Journal of thrombosis and haemostasis : JTH 18:2318-23282020Cationic zinc is required for factor XII recruitment and activation by stimulated platelets and for thrombus formation in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharjeel A Chaudhry,Matthew Serrata,Lindsay Tomczak,Sarah Higgins,Justine Ryu,Dylan Laprise,Keiichi Enjyoji,Roelof Bekendam,Virendar Kaushik,Robert Flaumenhaft,Pavan K BendapudiPubMed 32542960
- Cell reports 31:1077002020CTTNBP2 Controls Synaptic Expression of Zinc-Related Autism-Associated Proteins and Regulates Synapse Formation and Autism-like Behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu-Yun Shih,Bing-Yuan Hsieh,Ming-Hui Lin,Tzyy-Nan Huang,Ching-Yen Tsai,Wen-Li Pong,Sue-Ping Lee,Yi-Ping HsuehPubMed 32492416
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Assay time
15m
- Sensitivity
= 1 µg/mL
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Alternative names
Zn++, Zn2+
What's included?
Recommended products
Zinc Assay Kit ab102507 is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Zn++, Zn2+
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Assay time
15m
- Assay Platform
Microplate reader
- Sensitivity
= 1 µg/mL
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Zinc Assay Kit ab102507 is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm.
The zinc assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 μg/ml (~1-3 μM).
Zinc assay protocl summary:
- add samples and standards to wells
- add reaction mix and incubate for 10 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K387 Zinc Colorimetric Assay Kit. K387-100 is the same size as the 100 test size of ab102507.
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of 2+. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zinc a mineral essential for various biological functions has a molecular mass of 65.38 Da. It is not a protein but rather a metal ion that plays a mechanical role in many proteins and enzymes. Zinc is found throughout the human body in tissues such as the brain muscle bones kidneys and liver. It is also present in high concentrations in red and white blood cells eyes and the prostate. Zinc oxide a common form sees widespread use in industries including life sciences research for applications such as the liquid zinc assay.
- Biological function summary
Zinc acts as a cofactor in over 300 enzymatic reactions and is important for maintaining protein structure and function. It is part of zinc finger motifs helping in gene expression regulation by stabilizing the structure of these proteins. Zinc impacts cellular processes such as DNA repair RNA transcription and cellular signaling. It plays a role in immune system response wound healing and cell proliferation. As part of protein complexes it can bind to certain transcription factors affecting their activity and cellular response.
- Pathways
Zinc influences many significant biological pathways including those related to immune response and oxidative stress. It modulates the NF-kB signaling pathway important for immune system regulation. Furthermore zinc is involved in the antioxidant defense mechanism by supporting the function of enzymes like superoxide dismutase. These pathways are linked with important proteins such as metallothioneins which regulate zinc's cellular availability and detoxify heavy metals.
- Associated diseases and disorders
Zinc deficiency correlates with conditions such as immune dysfunction and growth retardation. Its imbalance is also linked to neurodegenerative disorders like Alzheimer’s disease where zinc interacts with amyloid-beta and tau proteins influencing disease progression. Zinc's interaction with proteins in these disorders suggests its levels need careful regulation to maintain health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Crawford, Aaron C et al., PLoS pathogens vol. 14,5 e1007013., Fig 2, doi:10.1371/journal.ppat.1007013Functional Studies - Zinc Assay Kit (ab102507)
Indicated strains were cultured in low zinc medium (SD0, pH ~4.7), exposed to 25 µM ZnSO4 and zinc acquisition determined at indicated time points by measuring how much zinc remained in the cell free supernatant. C. albicans wild type acquires all measurable zinc within 60 minute; zrt2Δ does not; complementation restored zinc acquisition to 68%. Experiment performed three times
Functional Studies - Zinc Assay Kit (ab102507)
Zinc measured in cell lysates showing quantity (nmol) per 1 mln of tested cells
Functional Studies - Zinc Assay Kit (ab102507)
Zinc measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Functional Studies - Zinc Assay Kit (ab102507)
Standard curve (colourimetric) : mean of duplicates (+/- SD) with background substracted
Functional Studies - Zinc Assay Kit (ab102507)
Example of Standard Curve.
Representative Standard Curve using ab102507.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com