JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB102507

Zinc Assay Kit (Colorimetric)

5

(1 Review)

|

(18 Publications)

Zinc Assay Kit ab102507 is a mix-and-read assay with a single 10-min incubation at room temperature. Readout on any colorimetric (560 nm) plate reader.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
5 Images
Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)
  • FuncS

Lab

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)

Zinc measured in cell lysates showing quantity (nmol) per 1 mln of tested cells

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)
  • FuncS

Supplier Data

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)

Example of Standard Curve.

Representative Standard Curve using ab102507.

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)
  • FuncS

Unknown

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)

Indicated strains were cultured in low zinc medium (SD0, pH ~4.7), exposed to 25 µM ZnSO4 and zinc acquisition determined at indicated time points by measuring how much zinc remained in the cell free supernatant. C. albicans wild type acquires all measurable zinc within 60 minute; zrt2Δ does not; complementation restored zinc acquisition to 68%. Experiment performed three times

Crawford, Aaron C et al., PLoS pathogens vol. 14,5 e1007013., Fig 2, doi:10.1371/journal.ppat.1007013

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)
  • FuncS

Lab

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)

Standard curve (colourimetric) : mean of duplicates (+/- SD) with background substracted

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)
  • FuncS

Lab

Functional Studies - Zinc Assay Kit (Colorimetric) (AB102507)

Zinc measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Results type

Quantitative

Sensitivity

= 1 µg/mL

Assay time

15m

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB176725

Zinc Assay Kit (Fluorometric)

Functional Studies - Zinc Assay Kit (Fluorometric) (AB176725)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB83366

Iron Assay Kit (Colorimetric)

Biochemical assay - Iron Assay Kit (Colorimetric) (AB83366)

  • 4
  • 3
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
style
left-column

Product details

Zinc Assay Kit ab102507 is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm.

The zinc assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 μg/ml (~1-3 μM).

Zinc assay protocol summary
- add samples and standards to wells
- add reaction mix and incubate for 10 min
- analyze with a microplate reader

Other Notes
This product was previously called K387 Biovision Zinc Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of 2+. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102507-100Test", "assetComponentDetails": [ { "size":"1 x 16 mL", "name":"Zinc Reagent 1", "number":"AB102507-CMP01", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Zinc Standard", "number":"AB102507-CMP03", "productcode":"" }, { "size":"1 x 4 mL", "name":"Zinc Reagent 2", "number":"AB102507-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"7% TCA", "number":"AB102507-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc a mineral essential for various biological functions has a molecular mass of 65.38 Da. It is not a protein but rather a metal ion that plays a mechanical role in many proteins and enzymes. Zinc is found throughout the human body in tissues such as the brain muscle bones kidneys and liver. It is also present in high concentrations in red and white blood cells eyes and the prostate. Zinc oxide a common form sees widespread use in industries including life sciences research for applications such as the liquid zinc assay.
Biological function summary

Zinc acts as a cofactor in over 300 enzymatic reactions and is important for maintaining protein structure and function. It is part of zinc finger motifs helping in gene expression regulation by stabilizing the structure of these proteins. Zinc impacts cellular processes such as DNA repair RNA transcription and cellular signaling. It plays a role in immune system response wound healing and cell proliferation. As part of protein complexes it can bind to certain transcription factors affecting their activity and cellular response.

Pathways

Zinc influences many significant biological pathways including those related to immune response and oxidative stress. It modulates the NF-kB signaling pathway important for immune system regulation. Furthermore zinc is involved in the antioxidant defense mechanism by supporting the function of enzymes like superoxide dismutase. These pathways are linked with important proteins such as metallothioneins which regulate zinc's cellular availability and detoxify heavy metals.

Zinc deficiency correlates with conditions such as immune dysfunction and growth retardation. Its imbalance is also linked to neurodegenerative disorders like Alzheimer's disease where zinc interacts with amyloid-beta and tau proteins influencing disease progression. Zinc's interaction with proteins in these disorders suggests its levels need careful regulation to maintain health.
style
left-column

Product protocols

style
left-column

Target data

See full target information ZINC
style
left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40362419

2025

Clinical Significance of Marginal Zinc Deficiency as a Predictor of Covert Hepatic Encephalopathy in Patients with Liver Cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Matsuda,Tadashi Namisaki,Akihiko Shibamoto,Shohei Asada,Fumimasa Tomooka,Takahiro Kubo,Aritoshi Koizumi,Misako Tanaka,Satoshi Iwai,Takashi Inoue,Yuki Tsuji,Yukihisa Fujinaga,Norihisa Nishimura,Shinya Sato,Koh Kitagawa,Kosuke Kaji,Akira Mitoro,Kiyoshi Asada,Hiroaki Takaya,Ryuichi Noguchi,Takemi Akahane,Hitoshi Yoshiji

Applied and environmental microbiology 90:e0148024 PubMed39360838

2024

Dietary zinc supplementation inhibits bacterial plasmid conjugation by regulating plasmid replication () and transfer () genes.

Applications

Unspecified application

Species

Unspecified reactive species

Logan Ott,Chloe Smith,Melha Mellata

Biomedicines 12: PubMed39062181

2024

Analysis of Ferritin, Hepcidin, Zinc, C-Reactive Protein and IL-6 Levels in COVID-19 in Patients Living at Different Altitudes in Peru.

Applications

Unspecified application

Species

Unspecified reactive species

Wilmer Silva-Caso,Sungmin Kym,Alfredo Merino-Luna,Miguel Angel Aguilar-Luis,Yordi Tarazona-Castro,Hugo Carrillo-Ng,Eliezer Bonifacio-Velez de Villa,Ronald Aquino-Ortega,Juana Del Valle-Mendoza

Protoplasma 261:487-496 PubMed38052957

2023

Elevated luteinizing hormone receptor signaling or selenium treatment leads to comparable changes in adrenal cortex histology and androgen-AR/ZIP9 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jaroslaw Wieczorek,Piotr Pawlicki,Marta Zarzycka,Laura Pardyak,Piotr Niedbala,Michal Duliban,Begum Yurdakok-Dikmen,Malgorzata Kotula-Balak

Cancer diagnosis & prognosis 2:184-193 PubMed35399181

2022

Clinical Significance of Serum Zinc Levels on the Development of Sarcopenia in Cirrhotic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Murata,Tadashi Namisaki,Yuki Fujimoto,Soichi Takeda,Masahide Enomoto,Hiroaki Takaya,Yuki Tsuji,Akihiko Shibamoto,Junya Suzuki,Takahiro Kubo,Satoshi Iwai,Fumimasa Tomooka,Misako Tanaka,Miki Kaneko,Shohei Asada,Aritoshi Koizumi,Nobuyuki Yorioka,Takuya Matsuda,Takahiro Ozutsumi,Koji Ishida,Hiroyuki Ogawa,Hirotetsu Takagi,Yukihisa Fujinaga,Masanori Furukawa,Yasuhiko Sawada,Norihisa Nishimura,Koh Kitagawa,Shinya Sato,Kosuke Kaji,Takashi Inoue,Kiyoshi Asada,Hideto Kawaratani,Kei Moriya,Takemi Akahane,Akira Mitoro,Hitoshi Yoshiji

Frontiers in immunology 12:785599 PubMed35058926

2022

Hypozincemia in COVID-19 Patients Correlates With Stronger Antibody Response.

Applications

Unspecified application

Species

Unspecified reactive species

Wenye Xu,Yingzhi Liu,Xuan Zou,Huanle Luo,Weihua Wu,Junjie Xia,Matthew T V Chan,Shisong Fang,Yuelong Shu,William K K Wu,Lin Zhang

Saudi journal of biological sciences 29:2958-2968 PubMed35531206

2022

Melatonin attenuates cerebral hypoperfusion-induced hippocampal damage and memory deficits in rats by suppressing TRPM7 channels.

Applications

Unspecified application

Species

Unspecified reactive species

Hussain Al Dera,Mohammed Alassiri,Reem Al Kahtani,Samy M Eleawa,Mohammad K AlMulla,Abdulhakeem Alamri

Cell reports 37:110132 PubMed34936871

2021

Resolving the immune landscape of human prostate at a single-cell level in health and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zewen Kelvin Tuong,Kevin W Loudon,Brendan Berry,Nathan Richoz,Julia Jones,Xiao Tan,Quan Nguyen,Anne George,Satoshi Hori,Sarah Field,Andy G Lynch,Katarzyna Kania,Paul Coupland,Anne Babbage,Richard Grenfell,Tristan Barrett,Anne Y Warren,Vincent Gnanapragasam,Charlie Massie,Menna R Clatworthy

Metabolic brain disease 37:299-310 PubMed34928474

2021

Impaired ion homeostasis as a possible associate factor in mucopolysaccharidosis pathogenesis: transcriptomic, cellular and animal studies.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Gaffke,Zuzanna Szczudło,Magdalena Podlacha,Zuzanna Cyske,Estera Rintz,Jagoda Mantej,Karolina Krzelowska,Grzegorz Węgrzyn,Karolina Pierzynowska

Journal of cellular and molecular medicine 25:8432-8441 PubMed34302427

2021

SLC39A5 dysfunction impairs extracellular matrix synthesis in high myopia pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Dong,Qi Tian,Tengfei Zhu,Kangli Wang,Ganting Lei,Yanling Liu,Haofeng Xiong,Lu Shen,Meng Wang,Rongjuan Zhao,Huidan Wu,Bin Li,Qiumeng Zhang,Yujun Yao,Hui Guo,Kun Xia,Lu Xia,Zhengmao Hu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com