AB176725

Zinc Assay Kit (Fluorometric)

Name: Zinc Assay Kit (Fluorometric) (ab176725) | Abcam
Brand: Abcam Limited
SKU: AB176725
Price: 495 USD
Availability: InStock

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(11 Publications)

View Alternative Names

Zn++, Zn2+

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Functional Studies - Zinc Assay Kit (Fluorometric) (AB176725)

FuncS

Supplier Data

Functional Studies - Zinc Assay Kit (Fluorometric) (AB176725)

ZnCl2 dose response curve.

ZnCl₂ dose response was measured on a 96-well black plate with Zinc Assay Kit (Fluorometric) (ab176725). As low as ~ 0.2 μM Zn²⁺ can be detected with 5 minutes incubation time (n=3).

Key facts

Detection method

Fluorescent

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

>= 13 ng/mL

Assay Platform

Microplate reader

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Product details

Zinc Assay Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn²⁺ (greater than 200~300 folds). It has high Zn²⁺ -specificity with little responses to other metals, including Ca²⁺, Mg²⁺, Mn²⁺, and Cu²⁺. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 μM (13 ng/mL).

Other Notes
This product was previously called Zinc Quantification Kit (Fluorometric).

Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176725-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Zn Detector", "number":"AB176725-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ZnCl2 Standard", "number":"AB176725-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Assay Buffer", "number":"AB176725-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc a mineral essential for various biological functions has a molecular mass of 65.38 Da. It is not a protein but rather a metal ion that plays a mechanical role in many proteins and enzymes. Zinc is found throughout the human body in tissues such as the brain muscle bones kidneys and liver. It is also present in high concentrations in red and white blood cells eyes and the prostate. Zinc oxide a common form sees widespread use in industries including life sciences research for applications such as the liquid zinc assay.

Biological function summary

Zinc acts as a cofactor in over 300 enzymatic reactions and is important for maintaining protein structure and function. It is part of zinc finger motifs helping in gene expression regulation by stabilizing the structure of these proteins. Zinc impacts cellular processes such as DNA repair RNA transcription and cellular signaling. It plays a role in immune system response wound healing and cell proliferation. As part of protein complexes it can bind to certain transcription factors affecting their activity and cellular response.

Pathways

Zinc influences many significant biological pathways including those related to immune response and oxidative stress. It modulates the NF-kB signaling pathway important for immune system regulation. Furthermore zinc is involved in the antioxidant defense mechanism by supporting the function of enzymes like superoxide dismutase. These pathways are linked with important proteins such as metallothioneins which regulate zinc's cellular availability and detoxify heavy metals.

Zinc deficiency correlates with conditions such as immune dysfunction and growth retardation. Its imbalance is also linked to neurodegenerative disorders like Alzheimer's disease where zinc interacts with amyloid-beta and tau proteins influencing disease progression. Zinc's interaction with proteins in these disorders suggests its levels need careful regulation to maintain health.

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Product protocols

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Target data

See full target information ZINC

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1272191 PubMed38116011

2023

Wound healing and Cadmium detoxification in the earthworm - a potential case for coelomocytes?

Applications

Unspecified application

Species

Unspecified reactive species

Gerhard P Aigner,Veronika Peer,Birgit Fiechtner,Cláudio Adriano Piechnik,Martina Höckner

Frontiers in pharmacology 14:1241406 PubMed37908978

2023

The antidepressant effect of short- and long-term zinc exposition is partly mediated by P2X7 receptors in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bernadett Iring-Varga,Mária Baranyi,Flóra Gölöncsér,Pál Tod,Beáta Sperlágh

Electrophoresis 44:217-245 PubMed35977346

2022

Microfluidic systems in clinical diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca H Lapizco-Encinas,Yan Victoria Zhang

Frontiers in nutrition 9:847910 PubMed35387197

2022

NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Olivares-González,David Salom,Emilio González-García,David Hervás,Natalia Mejía-Chiqui,Mar Melero,Sheyla Velasco,Bianca Tabita Muresan,Isabel Campillo,Nieves Vila-Clérigues,Eduardo López-Briz,Juan Francisco Merino-Torres,José María Millán,José Miguel Soriano Del Castillo,Regina Rodrigo

JMIR research protocols 10:e30909 PubMed34931994

2021

A Modern Flexitarian Dietary Intervention Incorporating Web-Based Nutrition Education in Healthy Young Adults: Protocol for a Randomized Controlled Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Braakhuis,Nicola Gillies,Anna Worthington,Scott Knowles,Tamlin Conner,Rajshri Roy,Toan Pham,Emma Bermingham,David Cameron-Smith

Reproductive biology 20:191-201 PubMed32245730

2020

Zinc deficient diet exacerbates the testicular and epididymal damage in type 2 diabetic rat: Studies on oxidative stress-related mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Krishna Prahlad Maremanda,Sarath Babu Srivalliputturu,Gopabandhu Jena

FASEB bioAdvances 2:49-58 PubMed32123856

2020

Active site plasticity and possible modes of chemical inhibition of the human DNA deaminase APOBEC3B.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Shi,Özlem Demir,Michael A Carpenter,Surajit Banerjee,Daniel A Harki,Rommie E Amaro,Reuben S Harris,Hideki Aihara

JOM (Warrendale, Pa. : 1989) 71:1436-1446 PubMed33731979

2019

Preclinical In-Vivo Evaluation and Screening of Zinc Based Degradable Metals for Endovascular Stents.

Applications

Unspecified application

Species

Unspecified reactive species

Roger J Guillory,Alexander A Oliver,Emma Davis,Elisha J Earley,Jaroslaw W Drelich,Jeremy Goldman

Developmental cell 46:441-455.e8 PubMed30100261

2018

PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Changfeng Li,Ying Zhang,Xing Cheng,Hua Yuan,Shan Zhu,Jiao Liu,Qirong Wen,Yangchun Xie,Jinbao Liu,Guido Kroemer,Daniel J Klionsky,Michael T Lotze,Herbert J Zeh,Rui Kang,Daolin Tang

Scientific reports 8:1329 PubMed29358714

2018

Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks.

Applications

Unspecified application

Species

Mouse

Sven Reinhardt,Nicolai Stoye,Mathias Luderer,Falk Kiefer,Ulrich Schmitt,Klaus Lieb,Kristina Endres

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Zinc Assay Kit (Fluorometric)

Functional Studies - Zinc Assay Kit (Fluorometric) (AB176725)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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