Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm.
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- Frontiers in immunology 14:12721912023Wound healing and Cadmium detoxification in the earthworm - a potential case for coelomocytes?Applications:Unspecified applicationReactive species:Unspecified reactive speciesGerhard P Aigner,Veronika Peer,Birgit Fiechtner,Cláudio Adriano Piechnik,Martina HöcknerPubMed 38116011
- Frontiers in pharmacology 14:12414062023The antidepressant effect of short- and long-term zinc exposition is partly mediated by P2X7 receptors in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBernadett Iring-Varga,Mária Baranyi,Flóra Gölöncsér,Pál Tod,Beáta SperlághPubMed 37908978
- Electrophoresis 44:217-2452022Microfluidic systems in clinical diagnosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlanca H Lapizco-Encinas,Yan Victoria ZhangPubMed 35977346
- Frontiers in nutrition 9:8479102022NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorena Olivares-González,David Salom,Emilio González-García,David Hervás,Natalia Mejía-Chiqui,Mar Melero,Sheyla Velasco,Bianca Tabita Muresan,Isabel Campillo,Nieves Vila-Clérigues,Eduardo López-Briz,Juan Francisco Merino-Torres,José María Millán,José Miguel Soriano Del Castillo,Regina RodrigoPubMed 35387197
- JMIR research protocols 10:e309092021A Modern Flexitarian Dietary Intervention Incorporating Web-Based Nutrition Education in Healthy Young Adults: Protocol for a Randomized Controlled Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Braakhuis,Nicola Gillies,Anna Worthington,Scott Knowles,Tamlin Conner,Rajshri Roy,Toan Pham,Emma Bermingham,David Cameron-SmithPubMed 34931994
- Reproductive biology 20:191-2012020Zinc deficient diet exacerbates the testicular and epididymal damage in type 2 diabetic rat: Studies on oxidative stress-related mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrishna Prahlad Maremanda,Sarath Babu Srivalliputturu,Gopabandhu JenaPubMed 32245730
- FASEB bioAdvances 2:49-582020Active site plasticity and possible modes of chemical inhibition of the human DNA deaminase APOBEC3B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Shi,Özlem Demir,Michael A Carpenter,Surajit Banerjee,Daniel A Harki,Rommie E Amaro,Reuben S Harris,Hideki AiharaPubMed 32123856
- JOM (Warrendale, Pa. : 1989) 71:1436-14462019Preclinical In-Vivo Evaluation and Screening of Zinc Based Degradable Metals for Endovascular Stents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoger J Guillory,Alexander A Oliver,Emma Davis,Elisha J Earley,Jaroslaw W Drelich,Jeremy GoldmanPubMed 33731979
- Developmental cell 46:441-455.e82018PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangfeng Li et. al.PubMed 30100261
- Scientific reports 8:13292018Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks.Applications:Unspecified applicationReactive species:MouseSven Reinhardt,Nicolai Stoye,Mathias Luderer,Falk Kiefer,Ulrich Schmitt,Klaus Lieb,Kristina EndresPubMed 29358714
Key facts
- Detection method
Fluorescent
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids
- Assay type
Quantitative
- Reactive species
Mammals
- Sensitivity
>= 13 ng/mL
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Target data
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Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm.
Key facts
- Detection method
Fluorescent
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids
- Assay type
Quantitative
- Reactive species
Mammals
- Assay Platform
Microplate reader
- Sensitivity
>= 13 ng/mL
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+ -specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 μM (13 ng/mL).
Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zinc a mineral essential for various biological functions has a molecular mass of 65.38 Da. It is not a protein but rather a metal ion that plays a mechanical role in many proteins and enzymes. Zinc is found throughout the human body in tissues such as the brain muscle bones kidneys and liver. It is also present in high concentrations in red and white blood cells eyes and the prostate. Zinc oxide a common form sees widespread use in industries including life sciences research for applications such as the liquid zinc assay.
- Biological function summary
Zinc acts as a cofactor in over 300 enzymatic reactions and is important for maintaining protein structure and function. It is part of zinc finger motifs helping in gene expression regulation by stabilizing the structure of these proteins. Zinc impacts cellular processes such as DNA repair RNA transcription and cellular signaling. It plays a role in immune system response wound healing and cell proliferation. As part of protein complexes it can bind to certain transcription factors affecting their activity and cellular response.
- Pathways
Zinc influences many significant biological pathways including those related to immune response and oxidative stress. It modulates the NF-kB signaling pathway important for immune system regulation. Furthermore zinc is involved in the antioxidant defense mechanism by supporting the function of enzymes like superoxide dismutase. These pathways are linked with important proteins such as metallothioneins which regulate zinc's cellular availability and detoxify heavy metals.
- Associated diseases and disorders
Zinc deficiency correlates with conditions such as immune dysfunction and growth retardation. Its imbalance is also linked to neurodegenerative disorders like Alzheimer’s disease where zinc interacts with amyloid-beta and tau proteins influencing disease progression. Zinc's interaction with proteins in these disorders suggests its levels need careful regulation to maintain health.
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Functional Studies - Zinc Quantification Kit (Fluorometric) (ab176725)
ZnCl2 dose response curve.
ZnCl2 dose response was measured on a 96-well black plate with Zinc Quantification Kit (Fluorometric) (ab176725). As low as ~ 0.2 μM Zn2+ can be detected with 5 minutes incubation time (n=3).
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