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Cobalt NTA Affinity Resin - Amintra (ab270018) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions.

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Key facts

Form
Liquid

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Cobalt NTA Affinity Resin - Amintra (ab270018) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions.

Key facts

Form
Liquid
Storage buffer

pH: 6 - 8
Constituents: Agarose beads, 20% Ethanol

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Cobalt NTA Affinity Resin - Amintra (ab270018) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Metal chelate affinity chromatography is a rapid one step purification, which removes most contaminants and can achieve purities close to homogeneity. The rapid purification protocols provided in the protocol book along with theNTA resin for affinity chromatography permit the recovery of high levels of pure recombinant protein in minutes. Large numbers of samples can be processed at the same time. Recombinant proteins purified using Cobalt NTA Affinity Resin - Amintra (ab270018) may be used in a wide range of structure and activity based laboratory procedures.

This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Cobalt NTA Affinity Resin - Amintra. Expedeon product codes ACO0010 (10 mL Medium), ACO0025 (25 mL Medium) and ACO0100 (100 mL Medium) are the same as the current sizes: 10 mL, 25 mL, and 100 mL, respectively.

Characterization of the resin

  • Supporting matrix: Highly cross-linked 6% agarose
  • Charged metal ion: Co2+
  • Bead size range: 45-165 μm
  • Recommended working pH Short term: pH 2.0-14.0 – Long term: pH 3.0-12.0
  • Typical binding capacity: > 20 mg 6x HIS-tagged recombinant protein/ml resin
  • Maximum pressure: 0.3MPa (3 bar)
  • Chemical stability: High
  • Solubility in water: Insoluble

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com