Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence)
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- Static binding capacity: >1mg DYKDDDDK fusion protein/mL medium
- Can be used to purify DYKDDDDK tag fusion proteins expressed in prokaryotes, yeast or mammalian cells
- Available in different sizes to fit your experiment needs
Product details
Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence) (ab270704) is an affinity chromatography medium designed for easy, one-step purification or immunoprecipitation of DYKDDDDK (flag) at N-terminal, C-terminal and internal locations of a fusion protein. Anti-DYKDDDDK Affinity Beads are coupled with an anti-DYKDDDDK monoclonal antibody by a technique which generates a stable linkage between the antibody and the agarose beads.
Characteristics of the resin:
| Item | Description |
| Matrix | 4% agarose beads |
| Ligand | Anti-DYKDDDDK Antibody |
| Binding Capacity | >1mg DYKDDDDK fusion protein/mL medium |
| Particle size (μm) | 45–165 |
| Max Pressure | 0.1 MPa, 1 bar |
| Storage Buffer | PBS buffer with 0.02% sodium azide |
| Storage Temperature | 4°C |
For the purification of DYKDDDDK-tagged fusion proteins, Anti-DYKDDDDK tag Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.
For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.
When a small amount of sample is being purified, immunoprecipitation procedure is recommended.
This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra® Anti-DYKDDDDK Affinity Beads. Expedeon product codes AF0001 (1 mL Medium), AF0005 (5 mL Medium), AF0010 (10mL Medium), AF0025 (25 mL Medium), and AF0050 (50 mL Medium) are the same as the current sizes: 1 mL, 5 mL, 10 mL, 25 mL, and 50 mL, respectively.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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