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AB270704

Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence)

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Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence) (ab270704) is an affinity chromatography medium designed for easy, one-step purification or immunoprecipitation of DYKDDDDK (flag) at N-terminal, C-terminal and internal locations of a fusion protein.

- Static binding capacity: >1mg DYKDDDDK fusion protein/mL medium
- Can be used to purify DYKDDDDK tag fusion proteins expressed in prokaryotes, yeast or mammalian cells
- Available in different sizes to fit your experiment needs

Key facts

Form

Liquid

form

Storage buffer

pH: 6 - 8 Preservative: 0.02% Sodium azide Constituents: 50% Agarose beads, 49.98% PBS

storage-buffer

Product details

Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence) (ab270704) is an affinity chromatography medium designed for easy, one-step purification or immunoprecipitation of DYKDDDDK (flag) at N-terminal, C-terminal and internal locations of a fusion protein. Anti-DYKDDDDK Affinity Beads are coupled with an anti-DYKDDDDK monoclonal antibody by a technique which generates a stable linkage between the antibody and the agarose beads.

Characteristics of the resin:

Item Description
Matrix 4% agarose beads
Ligand Anti-DYKDDDDK Antibody
Binding Capacity >1mg DYKDDDDK fusion protein/mL medium
Particle size (μm) 45–165
Max Pressure 0.1 MPa, 1 bar
Storage Buffer PBS buffer with 0.02% sodium azide
Storage Temperature 4°C

For the purification of DYKDDDDK-tagged fusion proteins, Anti-DYKDDDDK tag Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.

For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.

When a small amount of sample is being purified, immunoprecipitation procedure is recommended.


This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra® Anti-DYKDDDDK Affinity Beads. Expedeon product codes AF0001 (1 mL Medium), AF0005 (5 mL Medium), AF0010 (10mL Medium), AF0025 (25 mL Medium), and AF0050 (50 mL Medium) are the same as the current sizes: 1 mL, 5 mL, 10 mL, 25 mL, and 50 mL, respectively.

Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

Target data

websiteProtocolBooklet
en

Product promise

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