Anti-HA Affinity Resin - Amintra
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(3 Publications)
- Suitable for column chromatography or batch formats
- Available in different sizes to fit your experiment needs
Product details
Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.
The HA tag is derived from the human influenza virus HA protein corresponding to amino acids 98-106 (YPYDVPDYA). The HA tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein, so it has been extensively used as a general epitope tag at the N-terminal or C-terminal of the fusion protein. Anti-HA Affinity Resin - Amintra is based on a 4% agarose beads matrix, and non-specific binding is rare. It can be used for HA fusion protein purification and immunoprecipitation.
The characteristics of the resin:
| Item | Description |
| Matrix | 4% agarose beads |
| Ligand | Anti-HA Antibody |
| Binding capacity | >1mg HA fusion protein/mL medium |
| Particle Size (μm) | 45–165 |
| Max. Pressure | 0.1 MPa, 1 bar |
| Storage buffer | 1 x PBS with 0.02% sodium azide |
| Storage Temperature | 4°C |
For the purification of HA-tagged fusion proteins, Amintra Anti-HA Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.
For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.
When a small amount of sample is being purified, immunoprecipitation procedure is recommended.
Notes:
This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Anti-HA Affinity Beads. Expedeon product codes AHA0001 (1 mL medium) and AHA0005 (5 mL medium) are the same as the current sizes: 1 mL and 5 mL, respectively.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Publications (3)
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Carcinogenesis 46: PubMed39672957
2024
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Nature 621:620-626 PubMed37344598
2023
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The Journal of cell biology 220: PubMed33284320
2020
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Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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