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AB270603

Anti-HA Affinity Resin - Amintra

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(3 Publications)

Anti-HA Affinity Resin - Amintra (ab270603) is a ready-to-use affinity bead slurry designed for simple, rapid HA-tagged (YPYDVPDYA) recombinant protein purification or for immunoprecipitation (IP).

- Suitable for column chromatography or batch formats
- Available in different sizes to fit your experiment needs

Key facts

Form

Liquid

form

Storage buffer

pH: 6 - 8 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Agarose beads

storage-buffer

Product details

Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.

The HA tag is derived from the human influenza virus HA protein corresponding to amino acids 98-106 (YPYDVPDYA). The HA tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein, so it has been extensively used as a general epitope tag at the N-terminal or C-terminal of the fusion protein. Anti-HA Affinity Resin - Amintra is based on a 4% agarose beads matrix, and non-specific binding is rare. It can be used for HA fusion protein purification and immunoprecipitation.

The characteristics of the resin:

Item Description
Matrix 4% agarose beads
Ligand Anti-HA Antibody
Binding capacity >1mg HA fusion protein/mL medium
Particle Size (μm) 45–165
Max. Pressure 0.1 MPa, 1 bar
Storage buffer 1 x PBS with 0.02% sodium azide
Storage Temperature 4°C

For the purification of HA-tagged fusion proteins, Amintra Anti-HA Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.

For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.

When a small amount of sample is being purified, immunoprecipitation procedure is recommended.

Notes:

This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Anti-HA Affinity Beads. Expedeon product codes AHA0001 (1 mL medium) and AHA0005 (5 mL medium) are the same as the current sizes: 1 mL and 5 mL, respectively.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

Target data

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 46: PubMed39672957

2024

Testicular sex cord-stromal tumors in mice with constitutive activation of PI3K and loss of Pten.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Dinevska,Lachlan McAloney,Samuel S Widodo,Gulay Filiz,Jeremy Anderson,Sebastian Dworkin,Simon P Windley,Dagmar Wilhelm,Theo Mantamadiotis

Nature 621:620-626 PubMed37344598

2023

Structural basis of mitochondrial protein import by the TIM23 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Sue Im Sim,Yuanyuan Chen,Diane L Lynch,James C Gumbart,Eunyong Park

The Journal of cell biology 220: PubMed33284320

2020

The mitotic exit network regulates the spatiotemporal activity of Cdc42 to maintain cell size.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel M Gihana,Arthur A Cross-Najafi,Soni Lacefield
View all publications
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