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Ni- NTA Affinity Resin - Amintra (ab270549) is designed for simple, rapid His-tagged recombinant protein purification from cell lysates under native or denaturing conditions. Recover high levels of pure recombinant protein in minutes.

- Rapid one-step purification- removes most contaminants and can achieve purities close to homogeneity
- Large numbers of samples can be processed at the same time
- Can be used for recombinant proteins expressed in bacterial cells, Baculovirus vectors, mammalian cells or yeast

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Key facts

Form
Liquid

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Ni- NTA Affinity Resin - Amintra (ab270549) is designed for simple, rapid His-tagged recombinant protein purification from cell lysates under native or denaturing conditions. Recover high levels of pure recombinant protein in minutes.

- Rapid one-step purification- removes most contaminants and can achieve purities close to homogeneity
- Large numbers of samples can be processed at the same time
- Can be used for recombinant proteins expressed in bacterial cells, Baculovirus vectors, mammalian cells or yeast

Key facts

Form
Liquid
Storage buffer

pH: 6 - 8
Constituents: 20% Ethanol, 6% Agarose beads

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Ni- NTA Affinity Resin - Amintra (ab270549) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Metal chelate affinity chromatography is a rapid one-step purification, which removes most contaminants and can achieve purities close to homogeneity. The rapid purification protocols provided in this handbook for affinity chromatography permit the recovery of high levels of pure recombinant protein in minutes. Recombinant proteins purified using Ni- NTA Affinity Resin - Amintra may be used in a wide range of structure and activity-based laboratory procedures.

Immobilized Metal Affinity Chromatography (IMAC) technology was introduced by Porath et al (1975). The matrix is attached to chelating groups that immobilize transition metal ions such as Ni2+, Co2+, Cu2+, Zn2+ (Porath and Olin, 1983; Porath, 1988; Sulkowski, 1989). Certain amino acids such as histidine, tryptophan, cysteine and tyrosine can act as electron donors on the surface of the protein and bind reversibly to the transition metal ion. In the vast majority of instances, 6x histidine tag is engineered at the N or C terminus of the protein (Kd-10-13 at pH 8.0).

Ni2+ is the most widely used metal ion as most IMAC tags seem to have very high affinity for immobilized Ni2+. The simplicity of IMAC technology is extremely attractive as it lends itself to the bind, wash and elute mode of operation if the appropriate buffer formulations are selected. IMAC can often be used with samples without any pre-treatment e.g. buffer exchange step. The use of metal chelate affinity is widespread for the selective adsorption of engineered recombinant proteins and has largely superseded non-affinity methods of chromatography for purifying recombinant proteins.

Characteristics of the resin:

Supporting matrix

Highle cross-lined 6% agarose

Charged metal ion

Ni2+

Bead size range

45-165 μm

Recommended working pH

pH 2.0-12.0

Static binding capacity

>40 mg 6x His-tagged

Maximum pressure

0.3 MPa (3 bar)

Chemical stability

High

Solubility in water

Insoluble

This product is manufactured by Expedeon, an Abcam company and was previously called Amintra Ni-NTA Affinity Resin. Expedeon product codes ANN0010 (10 mL medium), ANN0025 (25 mL medium) and ANN0100 (100 mL) medium are the same as the current sizes: 10 mL, 25 mL and 100 mL respectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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