Protein G Sepharose®

Supplied as 50% slurry in 20% Ethanol.

Features:
High binding capacity: Binding of IgG >20 mg human or rabbit IgG/mL Protein G Sepharose^®.
Minimal Leaching of the Ligand
Flow Rate Tested*: 2.07 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Usage: Reusable for up to 10 times without significant loss of binding capacity.

These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.

Store beads at 4°C.

The beads may be damaged above 40°C.

DO NOT FREEZE.

Wash beads 3 times with 3x bead volume of desired buffer before use.

Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.

Sepharose is a registered trademark of GE Healthcare

This product is manufactured by BioVision, an Abcam company and was previously called 6511 Protein G Sepharose. 6511-100 is the same size as the 100 ml size of ab193259.

Protein G Sepharose^® beads are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose^® beads. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G Sepharose^® is >20 mg of human or rabbit IgG per mL of wet beads. Protein G Sepharose^® beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.