Explore our extensive range of assay kits, including metabolism assays, enzyme activity assays, and cell health and other cell-based assays. We manufacture over 40,000 ready-to-use, QC-validated biochemical and cell-based assay kits each year to save you time in in-house assay development.

Our assay kits are designed for use with colorimetric, fluorometric, and luminescent microplate readers, with samples such as plasma, urine, cell lysates, tissue extracts, and cultured cells.

Choose the right biochemical assay

Choosing the right biochemical assay is important. It affects what you measure, the quality, accuracy and reliability of your results, and whether they can be reproduced, significantly impacting the insights you gain from your research. The right biochemical assay also ensures specificity, sensitivity and compatibility with your sample type.

Likewise, the wrong biochemical assay can lead to false positives, resources being used on the wrong compounds, and a lack of reproducibility.

When choosing a biochemical assay, consider whether:

• It is biologically relevant
• It is sensitive enough to detect small changes
• It has the specificity to identify a true hit
• It matches your screening needs
• You’ll be able to mitigate any interference

All our assay kits are flexible; they can be used on multiple platforms and are adapted to automatable systems and liquid handlers, allowing you to get the most out of your high throughput screening and drug discovery.

Types of biochemical assays

Binding assays

Binding assay kits allow researchers to study and quantify the reaction between two molecules. They:

• Deliver high sensitivity and reproducibility
• Can be used with a variety of sample types, including protein-protein, protein-DNA, and receptor-ligand interactions
• Are available with fluorometric and colorimetric readers

Browse: DNA-Protein Binding Assay Kit (Colorimetric)andDNA-Protein Binding Assay Kit (Fluorometric)

Metabolism assays

Our metabolism assay kits are used to:

• Quantify the levels of metabolites, such as glycogen, glucose, and fatty acids
• Quantify the amount of enzyme activity for enzymes such as LDH and ATP synthase
• Analyze the state and throughput of metabolic processes and pathways, such as by measuring mitochondrial membrane potential and ATP levels
• Quantify enzyme activity and the levels of chemicals associated with oxidative stress, such as by measuring lipid peroxidation

Browse: DCFDA / H2DCFDA - Cellular ROS Assay Kit, Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)

Enzyme activity assays

In addition to a wide array of enzyme activity assay kits for metabolic enzymes, we also manufacture and supply assay kits for:

• Kinases
• Histone modification enzymes involved in epigenetics
• Cathepsins and caspases

and many other enzymes.

Our enzyme activity assay kits are based on substrates that, either directly after action by the enzyme or indirectly following action by a cascade of enzymes, produce a fluorometric or colorimetric readout.

Browse: Complex I Enzyme Activity Microplate Assay Kit (Colorimetric), Complex IV Rodent Enzyme Activity Microplate Assay Kit

Cell-based assays

Our cell health assays are designed to allow you to study cell viability, proliferation, cytotoxicity, and other fundamental cellular processes.

When treating cells or animals with experimental treatments, it's important to run cell health assays while measuring the levels of individual proteins and other cellular markers.

Our cell health assays include:

• Apoptosis assays
• Cytotoxicity assays and cell viability assays
• Cell proliferation assays
• Senescence assays
• Cell cycle assays
• Comet assays
• Live dead assays

Browse: Luminescent ATP Detection Assay Kit , MTT Assay Kit (Cell Proliferation)