By metabolic pathway

Metabolism assay kits can be selected based on the specific metabolic pathway or process you want to investigate. Choosing pathway-specific assays enables more targeted analysis and clearer interpretation of metabolic function.

Glycolysis and gluconeogenesis

Analyze key intermediates and enzymes involved in glucose metabolism to understand energy production, metabolic flux, and cellular responses to environmental changes.

Mitochondrial respiration

Assess mitochondrial function through measurements such as membrane potential, enzyme activity, and metabolite levels. These assays are critical for studying cellular energy production, apoptosis, and metabolic disorders.

Fatty acid and lipid metabolism

Quantify fatty acid oxidation, lipid synthesis, and eicosanoid signaling to investigate metabolic regulation, inflammation, and energy storage processes.

Oxidative stress and redox biology

Measure reactive oxygen species (ROS), antioxidant capacity, and redox balance to understand cellular stress responses and disease mechanisms.

Selecting assays by metabolic pathway helps ensure alignment between your experimental question and the biological process being studied.

By research area

Metabolism assay kits are widely used across multiple research and disease areas, where metabolic changes play a key role in cellular function and pathology.

Cancer research

Study metabolic reprogramming in tumor cells, including altered glycolysis (Warburg effect), mitochondrial function, and lipid metabolism.

Neurodegeneration

Investigate mitochondrial dysfunction, oxidative stress, and metabolic imbalances associated with conditions such as Alzheimer’s and Parkinson’s disease.

Diabetes and metabolic disorders

Analyze glucose metabolism, insulin signaling, and lipid regulation to better understand metabolic disease progression and therapeutic response.

Cardiovascular research

Assess energy metabolism, oxidative stress, and lipid pathways involved in heart function and vascular health.

Toxicology and drug discovery

Evaluate how compounds impact metabolic pathways, enabling safety assessment, mechanism-of-action studies, and compound screening.

Aligning assay selection with your application area helps ensure biologically relevant insights and more meaningful experimental outcomes.

Metabolism is a complex process that lies at the core of biology. Metabolic changes can lead to a huge range of outcomes in different disease areas, from cancer or neurodegeneration to obesity and diabetes. Our assay kits enable you to analyze the pathways of metabolism in-depth in live cells, lysates, and biofluids with easy analysis on your plate reader. With 20 years of experience in manufacturing metabolism assay kits and over 14,000 citations in scientific publications, we can help you understand your cell or disease model, or how your target or compound affects your metabolic pathway/phenotype of interest.

Benefits of our metabolism assays

• Easy-to-use with a simple step-by-step procedure included with each kit,  most kits do not require any wash steps .

• Consistent:  manufactured with strict QC criteria to ensure reproducible results and reliable long-term supply.

• Convenient: designed to deliver results with existing lab equipment, such as standard colorimetric and fluorometric plate readers, and with a range of sample types.

• Choice: a broad range of assays and reagents across the key analytes and targets within the most important metabolic pathways.

• Support assay scale-up: batch sizes up to 500 kits and individual assay components available for purchase to enable assay customization.

Explore our comprehensive range of metabolism assay kits that include mitochondrial assays, glycolysis assays, fatty acid assays, oxidative stress assays, and more to accelerate your metabolic research.

Mitochondrial assays

Measure mitochondrial health and function through changes in membrane potential. Our mitochondrial assay kits provide a variety of methods to assess mitochondrial function and to understand the function of mitochondria.

Glycolysis assays, gluconeogenesis, and glycogen

Detect and quantify key glycolysis intermediates for insights into energy production and metabolic pathways.

Fatty acid assays, lipids, and eicosanoid metabolism

Analyze the breakdown and synthesis of fatty acids in biological samples to gain insight into lipid metabolism.

Oxidative stress assays

Assess the balance between antioxidants and reactive oxygen species to study oxidative stress in various disease states.

Amino acid and nucleotide synthesis/metabolism

Find validated amino acid and nucleotide synthesis assay kits for glutamate, hypoxanthine, and purine metabolism. Compatible with high-throughput workflows.

Urea Cycle

Explore urea cycle assay kits for liver function and amino acid catabolism studies. Includes kits for quantifying ammonia, urea, citrulline, and arginine.