MW 364.4 Da, Purity >99%. DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.
64439-81-2
> 99%
Solid
364.4 Da
C20H16N2O5
4330531
Synthetic
MW 364.4 Da, Purity >99%. DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.
64439-81-2
> 99%
Solid
364.4 Da
C20H16N2O5
4330531
Synthetic
Soluble in DMSO to 50 mM.
(+/-)-10-Hydroxycamptothecin
DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.
CCC1(C2=C(COC1=O)C(=O)N3CC4=C(C3=C2)N=C5C=CC(=CC5=C4)O)O
InChI=1S/C20H16N2O5/c1-2-20(26)14-7-16-17-11(5-10-6-12(23)3-4-15(10)21-17)8-22(16)18(24)13(14)9-27-19(20)25/h3-7,23,26H,2,8-9H2,1H3
HAWSQZCWOQZXHI-UHFFFAOYSA-N
19-ethyl-7,19-dihydroxy-17-oxa-3,13-diazapentacyclo[11.8.0.02,11.04,9.015,20]henicosa-1(21),2(11),3,5,7,9,15(20)-heptaene-14,18-dione
Ambient - Can Ship with Ice
-20°C
-20°C
Store under desiccating conditions, The product can be stored for up to 12 months
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2D chemical structure image of ab141071, 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor
Anti-gamma H2A.X (phospho S139) antibody ab2893 staining γH2A.X in HeLa cells treated with 10-Hydroxycamptothecin (ab141071), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of 10-Hydroxycamptothecin, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141071 (10-Hydroxycamptothecin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-gamma H2A.X (phospho S139) antibody ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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