AB142227

10% NP-40 Substitute, Detergent. 10% Solution in Water.

Name: 10% NP-40 Substitute, Detergent. 10% Solution in Water. (CAS 2315-67-5) (ab142227) | Abcam
Brand: Abcam Limited
SKU: AB142227
Price: 95 USD
Availability: InStock

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(43 Publications)

10% NP-40 Substitute (CAS: 84133-50-6)(ab142227) is a non-ionic detergent that isolates membrane-bound proteins under non-denaturing conditions. Suitable to prepare cell lysis extracts and membrane protein isolation. Molecular weight (MW): 607.

- Suitable buffer reagent for your western blot workflow
- Supplied as ready-to-use 10% solution
- Alternative to NP-40 and Triton^® X-100 detergent solutions
- Proven performance: cited in over 40 publications
- Larger volume format available upon request

Key facts

CAS number

84133-50-6

Form

Liquid

form

Molecular weight

607 kDa

Molecular formula

C35H72O11

Nature

Synthetic

Biological description

Readily biodegradable non-ionic secondary ethoxylated alcohol surfactant that is used for cell lysis and membrane protein isolation. An alternative to NP-40 and other phenoxypolyethyloxyl-type detergents.

Canonical smiles

C(C(CCCCCCC)OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO[H])CCCCCC

InChi

InChI=1S/C35H72O11/c1-3-5-7-9-11-13-35(14-12-10-8-6-4-2)46-34-33-45-32-31-44-30-29-43-28-27-42-26-25-41-24-23-40-22-21-39-20-19-38-18-17-37-16-15-36/h35-36H,3-34H2,1-2H3

InChiKey

BHUIJDGAUFWHQT-UHFFFAOYSA-N

IUPAC Name

alkyl(C11-15)-secondary alcohol poly(oxyethylene)

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 2111 NP-40 (10% in H2O). 2111-100 is the same size as the 100 ML size of ab142227.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

The product can be stored for up to 12 months

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5222 PubMed40523893

2025

Coordinated action of multiple active histone modifications shapes the zygotic genome activation in teleost embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroto S Fukushima,Hiroyuki Takeda

STAR protocols 5:103460 PubMed39576731

2024

Protocol for evaluating extracellular matrix stiffness post-decellularization of triple-negative breast cancer cells using atomic force microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Swagata Adhikari,Manorama Ghosal,Aindrila Kabiraj,Dulal Senapati,Chandrima Das

Nature genetics 56:1701-1711 PubMed38744973

2024

GAGE-seq concurrently profiles multiscale 3D genome organization and gene expression in single cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tianming Zhou,Ruochi Zhang,Deyong Jia,Raymond T Doty,Adam D Munday,Daniel Gao,Li Xin,Janis L Abkowitz,Zhijun Duan,Jian Ma

Nature structural & molecular biology 31:1341-1354 PubMed38658621

2024

Alternative splicing of CARM1 regulated by LincGET-guided paraspeckles biases the first cell fate in mammalian early embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Yiwei Zhang,Jiaze Gao,Guihai Feng,Chao Liu,Xueke Li,Pengcheng Li,Zhonghua Liu,Falong Lu,Leyun Wang,Wei Li,Qi Zhou,Yusheng Liu

Molecular cell 84:1365-1376.e7 PubMed38452764

2024

Dissection of a CTCF topological boundary uncovers principles of enhancer-oncogene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Lock Kim,Gilbert J Rahme,Viraat Y Goel,Chadi A El Farran,Anders S Hansen,Bradley E Bernstein

Frontiers in microbiology 14:1281058 PubMed38075883

2023

Soft-metal(loid)s induce protein aggregation in .

Applications

Unspecified application

Species

Unspecified reactive species

Fabián A Cornejo,Claudia Muñoz-Villagrán,Roberto A Luraschi,María P Sandoval-Díaz,Camila A Cancino,Benoit Pugin,Eduardo H Morales,Jeff S Piotrowski,Juan M Sandoval,Claudio C Vásquez,Felipe A Arenas

Molecular metabolism 79:101850 PubMed38065435

2023

Evaluation of long acting GLP1R/GCGR agonist in a DIO and biopsy-confirmed mouse model of NASH suggest a beneficial role of GLP-1/glucagon agonism in NASH patients.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Monfeuga,Jenny Norlin,Anne Bugge,Elisabeth D Gaalsgaard,Cesar A Prada-Medina,Markus Latta,Sanne S Veidal,Pia S Petersen,Michael Feigh,Dorte Holst

Current protocols 3:e919 PubMed37987152

2023

Single-Nucleus RNA-Sequencing in Brain Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Waag,Johannes Bohacek

ACS omega 8:30578-30589 PubMed37636930

2023

Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffery T Jolly,Ty C Cheatham,Jessica S Blackburn

Frontiers in physiology 13:1064476 PubMed36589435

2022

Elevated antioxidant defence in the brain of deep-diving pinnipeds.

Applications

Unspecified application

Species

Unspecified reactive species

Gerrit A Martens,Lars P Folkow,Thorsten Burmester,Cornelia Geßner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

10% NP-40 Substitute, Detergent. 10% Solution in Water.

Key facts

CAS number

Form

Molecular weight

Molecular formula

Nature

Biological description

Canonical smiles

InChi

InChiKey

IUPAC Name

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Publications (43)

Product promise