2', 7'-Dichlorofluorescin diacetate

Key facts

CAS number

4091-99-0

Form

Solid

form

Molecular weight

487.3 Da

Molecular formula

C₂₄H₁₆Cl₂O₇

PubChem

77718

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Soluble in ethanol to 10 mM

Biochemical name

Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-

Biological description

Cell-permeable ROS probe. Reduced derivative of 2',7'-dichlorofluorescein. Can detect reactive oxygen species in live cells. Detects peroxidase activity (oxidative metabolism, intracellular regulation of ROS or oxidative burst). Upon enzyme activity, the highly fluorescent dye 2',7'-dichlorofluorescein is produced (Ex/Em = 495/529 nm).

Canonical smiles

CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl

InChi

InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChiKey

PXEZTIWVRVSYOK-UHFFFAOYSA-N

IUPAC Name

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

Excitation/Emission

Ex: 495nm, Em: 529nm