JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275920

2',7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG)

Be the first to review this product! Submit a review

|

(4 Publications)

MW 725.48 g/mol, Purity >98%. Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores. Absorption prior to enzyme hydrolysis is 290 nm.
1 Images
Chemical Structure - 2',7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG) (AB275920)
  • Chemical Structure

Lab

Chemical Structure - 2',7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG) (AB275920)

Chemical structure of 2',7'-Dichlorofluorescein di-beta-D-galactopyranoside (DCFDG)

Key facts

CAS number

17817-20-8

Purity

>98%

Form

Solid

form

Molecular weight

725.48 g/mol

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores. Absorption prior to enzyme hydrolysis is 290 nm.

Excitation/Emission

Ex: 495nm, Em: 529nm

style
left-column
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark|Store under desiccating conditions
style
left-column
style
left-column
style
left-column

Product protocols

style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 2:100340 PubMed33659904

2021

Analysis of lysosomal hydrolase trafficking and activity in human iPSC-derived neuronal models.

Applications

Unspecified application

Species

Unspecified reactive species

Leah K Cuddy,Joseph R Mazzulli

Analytical biochemistry 334:227-33 PubMed15494128

2004

Measurement of lysosomal glucocerebrosidase activity in mouse liver using a fluorescence-activated cell sorter assay.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Watt Chan,James Waire,Betsy Simons,Ken Karey,John Fung,Diane Copeland,Laura Andrews

Analytical biochemistry 247:268-71 PubMed9177687

1997

A flow cytometric assay for lysosomal glucocerebrosidase.

Applications

Unspecified application

Species

Unspecified reactive species

H H van Es,M Veldwijk,M Havenga,D Valerio

Biochemistry international 24:1135-44 PubMed1781792

1991

4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases.

Applications

Unspecified application

Species

Unspecified reactive species

E A Karpova, Voznyi YaV,T V Dudukina,I V Tsvetkova
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Biochemicals

AB275371

Resorufin maltotriose

Chemical Structure - Resorufin maltotriose (AB275371)

  • 0
  • 0
Primary Antibodies

AB267377

Anti-Wilms Tumor Protein antibody [EPR23963-116]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-Wilms Tumor Protein antibody [EPR23963-116] (AB267377)

  • 0
  • 0
Primary Antibodies

AB273052

Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free (AB273052)

  • 0
  • 0
Primary Antibodies

AB12723

Anti-Kaiso antibody [6F / 6F8]

Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (AB12723)

  • 5
  • 5

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com