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AB145256

5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate

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MW 460.4 Da, Purity >95%. Hydrophobic, cell-permeable fluorogenic non-selective esterase substrate. Becomes hydrolyzed to hydrophilic strongly fluorescent 5-Carboxyfluorescein (ab145293) intracellularly. Measures intracellular pH. 5-CFDA N-succinimidyl ester (ab145292) precursor.
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Chemical Structure - 5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate (AB145256)
  • Chemical Structure

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Chemical Structure - 5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate (AB145256)

2D chemical structure image of ab145256, 5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate

Key facts

CAS number

79955-27-4

Purity

>95%

Molecular weight

460.4 Da

Molecular formula

C<sub>2</sub><sub>5</sub>H<sub>1</sub><sub>6</sub>O<sub>9</sub>

PubChem

133314

Nature

Synthetic

Solubility

Soluble in ethanol to 10 mM

Soluble in DMSO

Biochemical name

5-Carboxyfluorescein diacetate

Biological description

Hydrophobic, cell-permeable fluorogenic non-selective esterase substrate. Becomes hydrolyzed to hydrophilic strongly fluorescent 5-Carboxyfluorescein (ab145293) intracellularly. Measures intracellular pH. 5-CFDA N-succinimidyl ester (ab145292) precursor.

Canonical smiles

CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C

InChi

InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChiKey

WPUZGNPQMIWOHE-UHFFFAOYSA-N

IUPAC Name

3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid

Excitation/Emission

Ex: 495nm, Em: 520nm

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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