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AB273895

5-(Iodoacetamido)fluorescein

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(3 Publications)

MW 515.3 g/mol, Purity >90%. Specifically and efficiently labels thiol groups on proteins. Fluorescein derivative sucessfully links to organelles, proteins and peptides at or near their physiological pH values (pH 7), including enzymes (e.g. (Na, K)-ATPase) or organelles like nucleii and nuclear matrices.
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Chemical Structure - 5-(Iodoacetamido)fluorescein (AB273895)
  • Chemical Structure

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Chemical Structure - 5-(Iodoacetamido)fluorescein (AB273895)

Chemical structure of 5-(Iodoacetamido)fluorescein, 63368-54-7

Key facts

CAS number

63368-54-7

Purity

>90%

Form

Solid

form

Molecular weight

515.3 g/mol

Molecular formula

C<sub>2</sub><sub>2</sub>H<sub>1</sub><sub>4</sub>INO<sub>6</sub>

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Specifically and efficiently labels thiol groups on proteins. Fluorescein derivative sucessfully links to organelles, proteins and peptides at or near their physiological pH values (pH 7), including enzymes (e.g. (Na, K)-ATPase) or organelles like nucleii and nuclear matrices.

IUPAC Name

N-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-2-iodoacetamide

Excitation/Emission

Ex: 490nm, Em: 520nm

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Biochemicals

AB145275

CPM, Thiol-reactive fluorescent probe

Chemical Structure - CPM, Thiol-reactive fluorescent probe (AB145275)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB24170

Anti-LAMP1 antibody - Lysosome Marker

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (AB24170)

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Primary Antibodies

AB220163

Anti-YTHDF2 antibody [EPR20318]

RabMAb|Recombinant|KO Validated

Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163)

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark|Store under desiccating conditions
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Product protocols

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 16:458-468 PubMed31819274

2019

Accurate annotation of human protein-coding small open reading frames.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas F Martinez,Qian Chu,Cynthia Donaldson,Dan Tan,Maxim N Shokhirev,Alan Saghatelian

Journal of thrombosis and haemostasis : JTH 17:1153-1159 PubMed30985986

2019

An assay to measure levels of factor Xa inhibitors in blood and plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Y Kim,Luca R Di Giuseppantonio,Chengliang Wu,James D Douketis,Peter L Gross

Methods in molecular biology (Clifton, N.J.) 1575:145-164 PubMed28255878

2017

Utilization of Selenocysteine for Site-Specific Antibody Conjugation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuling Li,Christoph Rader
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Product promise

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