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AB145258

5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label

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5(6)-FITC (CAS: 27072-45-3)(ab145258) is a amine reactive label. Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA. 5(6)-FITC DA (ab145463) precursor. 5-FITC (ab145325) and 6-FITC (ab146082) isomer mixture. MW 389.38, Purity >90%.

- Ex: 492 nm, Em: 518 nm
- Available in different sizes to fit your experimental needs. Larger volume format available upon request.
1 Images
Chemical Structure - 5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label (AB145258)
  • Chemical Structure

Lab

Chemical Structure - 5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label (AB145258)

2D chemical structure image of ab145258, 5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label

Key facts

CAS number

27072-45-3

Purity

>90%

Form

Powder

form

Molecular weight

389.38 g/mol

Molecular formula

C<sub>2</sub><sub>1</sub>H<sub>1</sub><sub>1</sub>NO<sub>5</sub>S

Nature

Synthetic

Solubility

Soluble in ethanol to 50 mM

Soluble in DMSO to 10 mM

Biological description

Green fluorescent, amine-reactive label. Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA. 5(6)-FITC DA (ab145463) precursor. 5-FITC (ab145325) and 6-FITC (ab146082) isomer mixture.

InChi

InChI=1S/C20H12O5.C2H3NS/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;1-3-2-4/h1-10,21-22H;1H3

InChiKey

QRQQVJDEAPQYJW-UHFFFAOYSA-N

IUPAC Name

Fluorescein 5(6)-isothiocyanate

Excitation/Emission

Ex: 492nm, Em: 518nm

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Product promise

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