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AB142391

7-Aminoactinomycin D (7-AAD), Fluorescent DNA dye

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(3 Publications)

MW 1270.4 Da, Purity >98%. Fluorescent DNA dye for apoptosis and flow cytometry studies. Guanine-specific synthetic fluorescent analogue of actinomycin D. Intercalates DNA by binding to GC regions. Discriminates between live cells as well as early and late apoptosis in flow cytometry. Excitation/emission = 488/647 nm.
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Chemical Structure - 7-Aminoactinomycin D (7-AAD), Fluorescent DNA dye (AB142391)
  • Chemical Structure

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Chemical Structure - 7-Aminoactinomycin D (7-AAD), Fluorescent DNA dye (AB142391)

2D chemical structure image of ab142391, 7-Aminoactinomycin D (7-AAD), Fluorescent DNA dye

Key facts

CAS number

7240-37-1

Purity

>98%

Form

Solid

form

Molecular weight

1270.4 Da

Molecular formula

C<sub>6</sub><sub>2</sub>H<sub>8</sub><sub>7</sub>N<sub>1</sub><sub>3</sub>O<sub>1</sub><sub>6</sub>

PubChem

65180

Nature

Synthetic

Solubility

Soluble in DMSO to 10 mM

Biochemical name

CID 65180

Biological description

Fluorescent DNA dye for apoptosis and flow cytometry studies. Guanine-specific synthetic fluorescent analogue of actinomycin D. Intercalates DNA by binding to GC regions. Discriminates between live cells as well as early and late apoptosis in flow cytometry. Excitation/emission = 488/647 nm.

Canonical smiles

CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)C(=C5O4)C)N)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)C)N

InChi

InChI=1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)

InChiKey

YXHLJMWYDTXDHS-UHFFFAOYSA-N

IUPAC Name

2,7-diamino-4,6-dimethyl-3-oxo-1-N,9-N-bis[7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide

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Complementary Products

Assay Kits

AB228563

7-AAD Staining Solution

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Assay Kits

AB14085

Annexin V-FITC Apoptosis Staining / Detection Kit

Functional Studies - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Reagents, Controls & Accessories

AB14083

Propidium Iodide

Immunofluorescence - Propidium Iodide (AB14083)

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  • 0
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39408757

2024

Vitamin B6 Pathway Maintains Glioblastoma Cell Survival in 3D Spheroid Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Najla Yussuf Moosa,Sara Abdullah Azeem,John K Lodge,William Cheung,Shafiq Uddin Ahmed

Frontiers in immunology 8:1063 PubMed28928739

2017

Gut REG3γ-Associated Induces Anti-inflammatory Macrophages to Maintain Adipose Tissue Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yugang Huang,HouBao Qi,Zhiqian Zhang,Enlin Wang,Huan Yun,Hui Yan,Xiaomin Su,Yingquan Liu,Zenzen Tang,Yunhuan Gao,Wencong Shang,Jiang Zhou,Tianze Wang,Yongzhe Che,Yuan Zhang,Rongcun Yang

Oncotarget 8:35728-35742 PubMed28415723

2017

Auranofin radiosensitizes tumor cells through targeting thioredoxin reductase and resulting overproduction of reactive oxygen species.

Applications

Species

Mouse

Hui Wang,Soumaya Bouzakoura,Sven de Mey,Heng Jiang,Kalun Law,Inès Dufait,Cyril Corbet,Valeri Verovski,Thierry Gevaert,Olivier Feron,Dirk Van den Berge,Guy Storme,Mark De Ridder
View all publications
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