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AB275548

7-(Diethylamino)coumarin-3-carbonyl azide

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(2 Publications)

MW 286.29 Da, Purity >95%. Aldehyde and ketone reactive probe. Suitable for labeling proteins and other carbonyl-containing molecules. Ceramides can be derivatized using 7-(diethylamino)coumarin-3-carbonyl azide with subsequent gradient HPLC separation allowing sensitive optical quantification of individual cellular ceramides. Suitable for use in HPLC labeling of lipids and alkylcycloketones.
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Chemical Structure - 7-(Diethylamino)coumarin-3-carbonyl azide (AB275548)
  • Chemical Structure

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Chemical Structure - 7-(Diethylamino)coumarin-3-carbonyl azide (AB275548)

Chemical structure of 7-(Diethylamino)coumarin-3-carbonyl azide, 157673-16-0

Key facts

CAS number

157673-16-0

Purity

>95%

Form

Solid

form

Molecular weight

286.29 Da

Molecular formula

C<sub>1</sub><sub>4</sub>H<sub>1</sub><sub>4</sub>N<sub>4</sub>O<sub>3</sub>

PubChem

4329619

Nature

Synthetic

Solubility

Soluble in DMSO.

Biochemical name

7-(Diethylamino)coumarin-3-carbonyl azide

Biological description

Aldehyde and ketone reactive probe. Suitable for labeling proteins and other carbonyl-containing molecules. Ceramides can be derivatized using 7-(diethylamino)coumarin-3-carbonyl azide with subsequent gradient HPLC separation allowing sensitive optical quantification of individual cellular ceramides. Suitable for use in HPLC labeling of lipids and alkylcycloketones.

Canonical smiles

CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)O2)C(=O)N=[N+]=[N-]

InChi

InChI=1S/C14H14N4O3/c1-3-18(4-2)10-6-5-9-7-11(13(19)16-17-15)14(20)21-12(9)8-10/h5-8H,3-4H2,1-2H3

InChiKey

FJIQMYGHCNXCHE-UHFFFAOYSA-N

IUPAC Name

7-(diethylamino)-2-oxochromene-3-carbonyl azide

Excitation/Emission

Ex: 390nm, Em: 478nm

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark|Store under desiccating conditions

Product protocols

Publications (2)

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Biochemistry 41:14010-8 PubMed12437358

2002

Interaction of tubulin with a new fluorescent analogue of vinblastine.

Applications

Unspecified application

Species

Unspecified reactive species

Sabarni K Chatterjee,Julien Laffray,Pruthvi Patel,Rudravajhala Ravindra,Yong Qin,Martin E Kuehne,Susan L Bane

Analytical biochemistry 233:145-50 PubMed8789711

1996

Measurement of platelet-activating factor acetylhydrolase activity by quantitative high-performance liquid chromatography determination of coumarin-derivatized 1-O-alkyl-2-sn-lysoglyceryl-3-phosphorylcholine.

Applications

Unspecified application

Species

Unspecified reactive species

C Balestrieri,G Camussi,A Giovane,E L Iorio,L Quagliuolo,L Servillo
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