MW 650.6 Da. Highly polar and water soluble lipase substrate derived from HPTS (pyranine), which forms tight micelles in aqueous solutions. Lipase activity can be established by measuring the fluorescence kinetics following the increase in emission of HPTS (hydroxypyrene trisulfonic acid). Due to its low pKa value (7.3), it is partially dissociated to form an anion that shows strong absorption (max: 460 nm) and maximum intensity at 520 nm. Fluorescence intensity is pH dependent between Ex:440-480 nm and reaches the isosbestic point at Ex: 415 nm.
MW 650.6 Da. Highly polar and water soluble lipase substrate derived from HPTS (pyranine), which forms tight micelles in aqueous solutions. Lipase activity can be established by measuring the fluorescence kinetics following the increase in emission of HPTS (hydroxypyrene trisulfonic acid). Due to its low pKa value (7.3), it is partially dissociated to form an anion that shows strong absorption (max: 460 nm) and maximum intensity at 520 nm. Fluorescence intensity is pH dependent between Ex:440-480 nm and reaches the isosbestic point at Ex: 415 nm.
Soluble in DMSO.
Soluble in water.
Highly polar and water soluble lipase substrate derived from HPTS (pyranine), which forms tight micelles in aqueous solutions. Lipase activity can be established by measuring the fluorescence kinetics following the increase in emission of HPTS (hydroxypyrene trisulfonic acid). Due to its low pKa value (7.3), it is partially dissociated to form an anion that shows strong absorption (max: 460 nm) and maximum intensity at 520 nm. Fluorescence intensity is pH dependent between Ex:440-480 nm and reaches the isosbestic point at Ex: 415 nm.
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Chemical structure of 8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, 115787-84-3
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