AM404, Anandamide transport inhibitor
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(1 Publication)
MW 395.6 Da, Purity >99%. Competitive and selective anandamide transport inhibitor (IC50 = 1 μM). Active in vivo. Does not activate CB1 receptors or inhibit anandamide hydrolysis but has been shown to activate vanilloid receptors.
View Alternative Names
(R)-limonene 6-monooxygenase, (S)-limonene 6-monooxygenase, (S)-limonene 7-monooxygenase, 1,8-cineole 2-exo-monooxygenase, 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE, 15 LOX 1, 15 lipoxygenase 1, 15-LOX, 15-PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD+], 70 kDa lysosomal alpha-glucosidase, AI838772, ALOX15, AW493413, Acid Maltase, Acid alpha glucosidase, Aglucosidase Alfa, Albendazole monooxygenase, Albendazole sulfoxidase, Alpha-glucosidase, Arachidonate 15-lipoxygenase, Arachidonate omega-6 lipoxygenase, CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1_HUMAN, CNR2_HUMAN, CNRII, CP2C9_HUMAN, CP33, CP34, CP3A4_HUMAN, CPC9, CX 5, CYP2C, CYP2C10, CYP3, CYP3A, CYP3A3, CYP3A4, CYPIIC9, CYPIIIA3, CYPIIIA4, Cannabinoid receptor 1, Cannabinoid receptor 2, Cannabinoid receptor 2 macrophage, Capsaicin receptor, Central cannabinoid receptor, Cytochrome P-450MP, Cytochrome P450 2C9, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 MP-4, Cytochrome P450 MP-8, Cytochrome P450 NF-25, Cytochrome P450 PB-1, Cytochrome P450 family 3 subfamily A polypeptide 4, Cytochrome P450 subfamily IIIA polypeptide 4, Cytochrome P450, family 2, subfamily C, polypeptide 9, Cytochrome P450-PCN1, DKFZp434K0220, FLJ11090, Glucocorticoid inducible P450, Glucosidase alpha, Glucosidase alpha acid, Glucosidase alpha acid (Pompe disease glycogen storage disease type II), HLP, Hpgd, Hydroxyprostaglandin dehydrogenase 15 (NAD), LOG15, LOX15_HUMAN, LYAG_HUMAN, Lysosomal Alpha-Glucosidase, MGC104252, MGC112732, MGC126680, MGC149605, MGC88320, Microsomal monooxygenase, NAD+ dependent 15 hydroxyprostaglandin dehydrogenase, NF 25, Nifedipine oxidase, OTRPC1, OTTHUMP00000016838, OTTHUMP00000020135, OTTHUMP00000214579, OTTHUMP00000218960, OTTHUMP00000219016, OTTHUMP00000219018, Osm-9-like TRP channel 1, P450 III steroid inducible, P450 MP, P450 PB 1, P450 PCN1, P450, family III, P450C3, P450IIC9, PGDH1, PGDH_HUMAN, PHOAR1, Prostaglandin dehydrogenase 1, Quinine 3-monooxygenase, RP24-311F12.2, S-mephenytoin 4-hydroxylase, SCAN1, SDR36C1, Short chain dehydrogenase/reductase family 36C member 1, TRPV1_HUMAN, TYDP, TYDP1_HUMAN, Taurochenodeoxycholate 6-alpha-hydroxylase, Transient receptor potential cation channel subfamily V member 1, Tyr-DNA phosphodiesterase 1, Tyrosyl-DNA phosphodiesterase 1, VR 1, Vanilloid receptor 1, Vanilloid receptor subtype 1, Xenobiotic monooxygenase, cytochrome P-450 S-mephenytoin 4-hydroxylase, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, flavoprotein-linked monooxygenase, hCB2, testis-dominant CNR2 isoform CB2
- FuncS
Unknown
Functional Studies - AM404, Anandamide transport inhibitor (AB120095)
SH-SY5Y cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and varied concentrations of AM404 (ab120095). Increased expression of c-Fos in SH-SY5Y cells correlates with an increase in AM404 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7963 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
- Chemical Structure
Lab
Chemical Structure - AM404, Anandamide transport inhibitor (AB120095)
2D chemical structure image of ab120095, AM404, Anandamide transport inhibitor
Properties and storage information
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Appropriate long-term storage conditions
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TRPV1 channels are central to detecting and regulating body temperature. They participate in inflammatory pain signaling. CB1 and CB2 receptors modulate neurotransmitter release and immune cell function. Both receptors have roles in the endocannabinoid system which includes endogenous cannabinoids like anandamide. CYP3A4 is critical for metabolizing xenobiotics and endogenous compounds affecting drug clearance rates. TDP1 helps in fixing DNA double-strand breaks working with other proteins in a complex. CYP2C9 is responsible for the oxidative metabolism of various substances including several drugs. 15-PGDH regulates prostaglandin levels and 15 Lipoxygenase 1 is involved in producing inflammatory mediators. GAA functions in lysosomes to convert glycogen to glucose.
Pathways
TRPV1 is part of the pain and sensory pathways including those associated with inflammatory responses. It interacts with proteins like calcineurin in pain signaling pathways. CB1 and CB2 receptors are integral to cannabinoid signaling pathways. They regulate the endocannabinoid system and affect pathways related to mood and appetite. CYP3A4 and CYP2C9 enzymes participate in the metabolic pathways of drug detoxification. 15-PGDH participates in arachidonic acid metabolism relating to the pathway of prostaglandin degradation. 15 Lipoxygenase 1 associated with linoleic acid metabolism contributes to leukotriene production. GAA is significant in glycogen metabolism pathways related to energy release processes.
Publications (1)
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Nature neuroscience 13:592-600 PubMed20348918
2010
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Species
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