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AB120495

Anisomycin, Protein synthesis inhibitor

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(4 Publications)

MW 265.3 Da, Purity >98%. Translational inhibitor of protein synthesis. Highly potent activator of kinase cascades. Potently activates stress-activated protein kinases, p38, JNK MAP and other kinases.
2 Images
Immunocytochemistry/ Immunofluorescence - Anisomycin, Protein synthesis inhibitor (AB120495)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anisomycin, Protein synthesis inhibitor (AB120495)

ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - Anisomycin, Protein synthesis inhibitor (AB120495)
  • Chemical Structure

Lab

Chemical Structure - Anisomycin, Protein synthesis inhibitor (AB120495)

2D chemical structure image of ab120495, Anisomycin, Protein synthesis inhibitor

Key facts

CAS number

27958-09-4

Purity

>98%

Form

Solid

form

Source

Streptomyces griseolus

Molecular weight

265.3 Da

Molecular formula

C<sub>1</sub><sub>4</sub>H<sub>1</sub><sub>9</sub>NO<sub>4</sub>

PubChem

31549

Nature

Native

Solubility

Soluble in DMSO to 100 mM

Soluble in ethanol to 100 mM

Biochemical name

3,4-Pyrrolidinediol, 2-(p-methoxybenzyl)-, 3-acetate, (2S,3R,4R)-

Biological description

Translational inhibitor of protein synthesis. Highly potent activator of kinase cascades. Potently activates stress-activated protein kinases, p38, JNK MAP and other kinases.

Canonical smiles

CC(=O)OC1C(CNC1CC2=CC=C(C=C2)OC)O

Isomeric smiles

CC(=O)O[C@H]1[C@@H](CN[C@H]1CC2=CC=C(C=C2)OC)O

InChi

InChI=1S/C14H19NO4/c1-9(16)19-14-12(15-8-13(14)17)7-10-3-5-11(18-2)6-4-10/h3-6,12-15,17H,7-8H2,1-2H3/t12-,13+,14+/m0/s1

InChiKey

YKJYKKNCCRKFSL-BFHYXJOUSA-N

IUPAC Name

[(2S,3R,4R)-4-hydroxy-2-[(4-methoxyphenyl)methyl]pyrrolidin-3-yl] acetate

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed33781382

2021

All-trans retinoic acid induces synaptic plasticity in human cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Lenz,Pia Kruse,Amelie Eichler,Jakob Straehle,Jürgen Beck,Thomas Deller,Andreas Vlachos

Neoplasia (New York, N.Y.) 19:354-363 PubMed28319809

2017

ABT-737 Synergizes with Cisplatin Bypassing Aberration of Apoptotic Pathway in Non-small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Young Kim,Ji Ye Jung,Arum Kim,Yoon Soo Chang,Se Kyu Kim

Scientific reports 5:8733 PubMed25736434

2015

Mesenchymal stem cells alleviate airway inflammation and emphysema in COPD through down-regulation of cyclooxygenase-2 via p38 and ERK MAPK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Gu,Lin Song,Xiao-Ming Li,Di Wang,Xue-Jun Guo,Wei-Guo Xu

Science signaling 6:ra81 PubMed24023255

2013

Ajuba family proteins link JNK to Hippo signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Gongping Sun,Kenneth D Irvine
View all publications
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