Anisomycin, Protein synthesis inhibitor

2 product images

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  Chemical Structure - Anisomycin, Protein synthesis inhibitor (ab120495)

  2D chemical structure image of ab120495, Anisomycin, Protein synthesis inhibitor

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  Immunocytochemistry/ Immunofluorescence - Anisomycin, Protein synthesis inhibitor (ab120495)

  Anti-Tubulin antibody [YOL1/34] - Loading Control ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
  The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-Tubulin antibody [YOL1/34] - Loading Control ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.