ATP-gamma-S is a kinase substrate and inhibitor of ATP-dependent enzyme systems. Hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. P2 purinergic receptor agonist.
- Cited in over 30 publications
- Purity > 80%
- Soluble in water to 10 mM
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23023682023Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaiyuan Liang,Chunguang Yang,Ruijiang Zeng,Yingqiu Song,Jianxi Wang,Wei Xiong,Binyuan Yan,Xin JinPubMed 37949681
- International journal of molecular sciences 24:2023Fyn-Mediated Paxillin Tyrosine 31 Phosphorylation Regulates Migration and Invasion of Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhang,Huanyu Zheng,Ming Xu,Noriko Maeda,Ryouichi Tsunedomi,Hiroko Kishi,Hiroaki Nagano,Sei KobayashiPubMed 37958964
- aBIOTECH 4:185-2012023DNA methylation-dependent epigenetic regulation of virulence in plants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Ya Chen,Chen Zhu,Jian-Hua Zhao,Ting Liu,Feng Gao,Ying-Chao Zhang,Cheng-Guo DuanPubMed 37970467
- International journal of molecular sciences 24:2023OsMKK6 Regulates Disease Resistance in Rice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuirui Jiang,Shichen Zhou,Xiaowen Da,Peng Yan,Kai Wang,Jiming Xu,Xiaorong MoPubMed 37628859
- Cell death & disease 14:4692023Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiufeng Chu,Xiang Xiao,Guangchuan Wang,Ahmed Uosef,Xiaohua Lou,Preston Arnold,Yixuan Wang,Gangcheng Kong,Mou Wen,Laurie J Minze,Xian C LiPubMed 37495617
- Nature communications 14:28592023ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangling Xiao,Jie Shi,Chuan He,Xia Bu,Yishuang Sun,Minling Gao,Bolin Xiang,Wenjun Xiong,Panpan Dai,Qi Mao,Xixin Xing,Yingmeng Yao,Haisheng Yu,Gaoshan Xu,Siqi Li,Yan Ren,Baoxiang Chen,Congqing Jiang,Geng Meng,Yu-Ru Lee,Wenyi Wei,Gordon J Freeman,Conghua Xie,Jinfang ZhangPubMed 37208329
- Molecular & cellular proteomics : MCP 22:1005222023Chemical Genetic Identification of PKC Epsilon Substrates in Mouse Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael P Dugan,Laura B Ferguson,Nicholas T Hertz,Robert J Chalkley,Alma L Burlingame,Kevan M Shokat,Peter J Parker,Robert O MessingPubMed 36863607
- Journal of lipid research 63:1002792022ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsli D Yildirim,Mevlut Citir,Asli E Dogan,Zehra Veli,Zehra Yildirim,Ozlem Tufanli,Alexis Traynor-Kaplan,Carsten Schultz,Ebru ErbayPubMed 36100091
- mBio 13:e00971222022Ubiquitination of SARS-CoV-2 NSP6 and ORF7a Facilitates NF-κB Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHironori Nishitsuji,Satoko Iwahori,Mariko Ohmori,Kunitada Shimotohno,Takayuki MurataPubMed 35856559
- Journal of virology 96:e00107222022Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Chiao Yu,Pei-Chen Lin,Cho-Han Chiang,Shu-Tang Jen,Yen-Ling Lai,Shih-Chin Hsu,Lee-Chiang Lo,Jing-Jer Lin,Nei-Li Chan,Ming-Jiun YuPubMed 35293767
Key facts
- CAS number
- 93839-89-5
- Purity
- > 80%
- Form
- Solid
- Molecular weight
- 547.1 Da
- Molecular formula
- C10H12Li4N5O12P3S
- PubChem identifier
- 5311341
- Nature
- Synthetic
Target data
Additional Targets
ATP gamma S
Alternative names
ATP g S, Adenosine 5' (3 thiotriphosphate)tetralithium salt, Ado 5' PPP[S]
Recommended products
ATP-gamma-S is a kinase substrate and inhibitor of ATP-dependent enzyme systems. Hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. P2 purinergic receptor agonist.
- Cited in over 30 publications
- Purity > 80%
- Soluble in water to 10 mM
Key facts
- CAS number
- 93839-89-5
- Purity
- > 80%
- Form
- Solid
- Molecular weight
- 547.1 Da
- Molecular formula
- C10H12Li4N5O12P3S
- PubChem identifier
- 5311341
- Nature
- Synthetic
- Solubility
Soluble in water to 10 mM.
- Biochemical name
- Adenosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid, tetralithium salt
- Biological description
Substrate and inhibitor of ATP-dependent enzyme systems. Hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. P2 purinergic receptor agonist.
- Canonical SMILES
- [Li+].[Li+].[Li+].[Li+].C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)([O-])OP(=O)([O-])OP(=S)([O-])[O-])O)O)N
- Isomeric SMILES
- [Li+].[Li+].[Li+].[Li+].C1=NC(=C2C(=N1)N(C=N2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)([O-])OP(=O)([O-])OP(=S)([O-])[O-])O)O)N
- InChI
- InChI=1S/C10H16N5O12P3S.4Li/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(25-10)1-24-28(18,19)26-29(20,21)27-30(22,23)31;;;;/h2-4,6-7,10,16-17H,1H2,(H,18,19)(H,20,21)(H2,11,12,13)(H2,22,23,31);;;;/q;4*+1/p-4/t4-,6-,7-,10-;;;;/m1..../s1
- InChIKey
- DWQFDOIBOYDYKH-KWIZKVQNSA-J
- IUPAC name
- tetralithium;[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl] dioxidophosphinothioyl phosphate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Notes
Kinase reaction reagent to be used with Anti-Thiophosphate ester antibody [51-8] ab92570 rabbit monoclonal to thiophosphate ester for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).
After the kinase of interest has accepted ATP-γ-S, p-Nitrobenzyl mesylate (p-Nitrobenzyl mesylate, Alkylation reagent ab138910) can be used to alkylate the thiophospholyation site on the substrates. A thiophosphate ester rabbit monoclonal antibody (Anti-Thiophosphate ester antibody [51-8] ab92570) is introduced to identify the tagged substrates.
For western blot analysis, we used ATPγS or A*TPγS analogs at a concentration of 1 mM. For kinetic measurements, ATPγS or A*TPγS analog concentration varied from 0.1 μM to 250 μM. See Allen JJ et al. Nat Methods 4:511-6 (2007); Supp. Material.
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Chemical Structure - ATP-gamma-S, Kinase substrate (ab138911)
2D chemical structure image of ab138911, ATP-gamma-S, Kinase substrate
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