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AB120503

BAPTA-AM, Ca2+ chelator

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(33 Publications)

MW 764.7 Da, Purity >97%. Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
2 Images
Immunocytochemistry/ Immunofluorescence - BAPTA-AM, Ca2+ chelator (AB120503)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - BAPTA-AM, Ca2+ chelator (AB120503)

ab66705 staining PAI1 in HepG2 cells treated with BAPTA-AM (ab120503), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of BAPTA-AM, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120503 (BAPTA-AM) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - BAPTA-AM, Ca2+ chelator (AB120503)
  • Chemical Structure

Lab

Chemical Structure - BAPTA-AM, Ca2+ chelator (AB120503)

2D chemical structure image of ab120503, BAPTA-AM, Ca2+ chelator

Key facts

CAS number

126150-97-8

Purity

>97%

Form

Solid

form

Molecular weight

764.7 Da

Molecular formula

C<sub>3</sub><sub>4</sub>H<sub>4</sub><sub>0</sub>N<sub>2</sub>O<sub>1</sub><sub>8</sub>

PubChem

2293

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

Bapta-AM

Biological description

Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).

Canonical smiles

CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi

InChI=1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3

InChiKey

YJIYWYAMZFVECX-UHFFFAOYSA-N

IUPAC Name

acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]anilino]acetate

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Complementary Products

Biochemicals

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Functional Studies - 2-APB, Ca2+ release modulator (AB120124)

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Biochemicals

AB120286

Thapsigargin, Ca2+-ATPase inhibitor

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Biochemicals

AB142773

Fluo-8 AM, green fluorescent calcium binding dye

Fluorescent Cell Imaging - Fluo-8 AM, green fluorescent calcium binding dye (AB142773)

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  • 1
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDP1 also known as Tyrosyl-DNA Phosphodiesterase 1 or TDP1p is an enzyme that plays an important role in DNA repair. It has a molecular mass of approximately 67 kDa and is expressed ubiquitously across various human tissues suggesting its fundamental role in maintaining genomic stability. Mechanically TDP1 acts by cleaving the phosphodiester bond between a tyrosine residue and a DNA 3' terminus which is important in resolving protein-DNA complexes that result from DNA damage.
Biological function summary

This enzyme is indispensable in the repair of topoisomerase I-mediated DNA damage participating as an important player in the DNA repair machinery. TDP1 works within the context of a complex involving other repair proteins ensuring the removal of DNA-protein cross-links that could otherwise lead to cell death if left unrepaired. This activity highlights its role in genomic stability and cell viability.

Pathways

TDP1 finds its place within the DNA single-strand break repair and the DNA damage response pathways. Its function is closely associated with other proteins such as XRCC1 which enhances the stability and efficiency of the DNA repair process and PARP1 which directs the cell's response to DNA damage. These interactions highlight TDP1's role in safeguarding genetic information by enabling precise and effective repair mechanisms.

TDP1 is notably implicated in neurodegenerative conditions such as Spinocerebellar Ataxia with Axonal Neuropathy (SCAN1) demonstrating how critical its proper function is for neuronal survival. Mutations or dysregulation in TDP1 have also been linked to heightened sensitivity to chemotherapeutic agents indicating potential challenges in cancer treatment. These disease linkages put TDP1 in association with proteins like ATM and BRCA1 which are involved in cellular responses to DNA damage and maintaining genomic integrity.
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Product protocols

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Biological research 58:3 PubMed39810241

2025

Protein palmitoylation is involved in regulating mouse sperm motility via the signals of calcium, protein tyrosine phosphorylation and reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping Xiong,Chenchen Yi,Haixia Zheng,Ya Ni,Yamei Xue,Kun Li

Biotechnology and applied biochemistry 72:104-115 PubMed39113388

2024

HPV18 E6/E7 activates Ca influx to promote the malignant progression of cervical cancer by inhibiting Ca binding protein 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Kang,Lei Qiu,Yali Duo,FengLing Bi,Zhongjie Liu,Jing Wang,Lei Zheng,Ning Zhao

Bio-protocol 14:e4959 PubMed38841288

2024

Dissecting the Mechanical Control of Mitotic Entry Using a Cell Confinement Setup.

Applications

Unspecified application

Species

Unspecified reactive species

Margarida Dantas,Débora Vareiro,Jorge G Ferreira

British journal of pharmacology 181:2725-2749 PubMed38637276

2024

Chymotrypsin activity signals to intestinal epithelium by protease-activated receptor-dependent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Guignard,Mahmoud Saifeddine,Koichiro Mihara,Majid Motahhary,Magali Savignac,Laura Guiraud,David Sagnat,Mireille Sebbag,Sokchea Khou,Corinne Rolland,Anissa Edir,Barbara Bournet,Louis Buscail,Etienne Buscail,Laurent Alric,Caroline Camare,Mouna Ambli,Nathalie Vergnolle,Morley D Hollenberg,Céline Deraison,Chrystelle Bonnart

Cell communication and signaling : CCS 22:92 PubMed38303059

2024

T-type voltage-gated channels, Na/Ca-exchanger, and calpain-2 promote photoreceptor cell death in inherited retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yan,Lan Wang,Qian-Lu Yang,Qian-Xi Yang,Xinyi He,Yujie Dong,Zhulin Hu,Mathias W Seeliger,Kangwei Jiao,François Paquet-Durand

The Journal of cell biology 222: PubMed36946995

2023

The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E L Garner,Anna Salter,Clinton K Lau,Manickam Gurusaran,Cécile M Villemant,Elizabeth P Granger,Gavin McNee,Philip G Woodman,Owen R Davies,Brian E Burke,Victoria J Allan

Molecular neurobiology 60:1580-1593 PubMed36526930

2022

Muscarinic Receptors in Developmental Axonal Competition at the Neuromuscular Junction.

Applications

Unspecified application

Species

Unspecified reactive species

Josep Tomàs,Maria A Lanuza,Manel M Santafé,Víctor Cilleros-Mañé,Laia Just-Borràs,Marta Balanyà-Segura,Aleksandra Polishchuk,Laura Nadal,Marta Tomàs,Neus Garcia

The Journal of cell biology 221: PubMed36222828

2022

Nuclear tension controls mitotic entry by regulating cyclin B1 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Margarida Dantas,Andreia Oliveira,Paulo Aguiar,Helder Maiato,Jorge G Ferreira

Cellular and molecular life sciences : CMLS 79:495 PubMed36001172

2022

Cdk5 regulates IP3R1-mediated Ca dynamics and Ca-mediated cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Saranya NavaneethaKrishnan,Vincent Law,Jungkwon Lee,Jesusa L Rosales,Ki-Young Lee

Cell reports 38:110255 PubMed35045279

2022

Presynaptic mechanisms underlying GABA-receptor-mediated inhibition of spontaneous neurotransmitter release.

Applications

Unspecified application

Species

Unspecified reactive species

Baris Alten,Natalie J Guzikowski,Zack Zurawski,Heidi E Hamm,Ege T Kavalali
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