MW 764.7 Da, Purity >97%. Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
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- The Journal of cell biology 222:2023The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKirsten E L Garner,Anna Salter,Clinton K Lau,Manickam Gurusaran,Cécile M Villemant,Elizabeth P Granger,Gavin McNee,Philip G Woodman,Owen R Davies,Brian E Burke,Victoria J AllanPubMed 36946995
- Molecular neurobiology 60:1580-15932022Muscarinic Receptors in Developmental Axonal Competition at the Neuromuscular Junction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosep Tomàs,Maria A Lanuza,Manel M Santafé,Víctor Cilleros-Mañé,Laia Just-Borràs,Marta Balanyà-Segura,Aleksandra Polishchuk,Laura Nadal,Marta Tomàs,Neus GarciaPubMed 36526930
- The Journal of cell biology 221:2022Nuclear tension controls mitotic entry by regulating cyclin B1 nuclear translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargarida Dantas,Andreia Oliveira,Paulo Aguiar,Helder Maiato,Jorge G FerreiraPubMed 36222828
- Cellular and molecular life sciences : CMLS 79:4952022Cdk5 regulates IP3R1-mediated Ca dynamics and Ca-mediated cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaranya NavaneethaKrishnan,Vincent Law,Jungkwon Lee,Jesusa L Rosales,Ki-Young LeePubMed 36001172
- Cell reports 38:1102552022Presynaptic mechanisms underlying GABA-receptor-mediated inhibition of spontaneous neurotransmitter release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaris Alten,Natalie J Guzikowski,Zack Zurawski,Heidi E Hamm,Ege T KavalaliPubMed 35045279
- Nature communications 12:69672021Compressive stress-mediated p38 activation required for ERα + phenotype in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPauliina M Munne,Lahja Martikainen,Iiris Räty,Kia Bertula, Nonappa,Janika Ruuska,Hanna Ala-Hongisto,Aino Peura,Babette Hollmann,Lilya Euro,Kerim Yavuz,Linda Patrikainen,Maria Salmela,Juho Pokki,Mikko Kivento,Juho Väänänen,Tomi Suomi,Liina Nevalaita,Minna Mutka,Panu Kovanen,Marjut Leidenius,Tuomo Meretoja,Katja Hukkinen,Outi Monni,Jeroen Pouwels,Biswajyoti Sahu,Johanna Mattson,Heikki Joensuu,Päivi Heikkilä,Laura L Elo,Ciara Metcalfe,Melissa R Junttila,Olli Ikkala,Juha KlefströmPubMed 34845227
- Annals of translational medicine 9:14702021Cold-inducible RNA-binding protein migrates from the nucleus to the cytoplasm under cold stress in normal human bronchial epithelial cells via TRPM8-mediated mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Ping Mao,Yan Jiao,Jian-Hua Xiang,Xin-Wei Luo,Qian He,Dan-Hua Ran,Qing Xu,Chun-Hui Lang,Ling-Xiu ChenPubMed 34734022
- Molecular systems biology 17:e104802021Multistability maintains redox homeostasis in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJo-Hsi Huang,Hannah Kc Co,Yi-Chen Lee,Chia-Chou Wu,Sheng-Hong ChenPubMed 34612597
- Molecular cell 81:3509-3525.e52021Nascent RNA scaffolds contribute to chromosome territory architecture and counter chromatin compaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin Michael Creamer,Heather Jill Kolpa,Jeanne Bentley LawrencePubMed 34320406
- Archives of toxicology 95:617-6302020Synthetic cannabinoid CP-55,940 induces apoptosis in a human skeletal muscle model via regulation of CB receptors and L-type Ca channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKen-Ichi Tomiyama,Masahiko FunadaPubMed 33174160
Key facts
- CAS number
- 126150-97-8
- Purity
- > 97%
- Form
- Solid
- Molecular weight
- 764.7 Da
- Molecular formula
- C34H40N2O18
- PubChem identifier
- 2293
- Nature
- Synthetic
Target data
Alternative names
AI838772, AW493413, FLJ11090, Hypothetical protein FLJ35932, MGC104252, MGC112732, RP24-311F12.2, SCAN1, STK33_HUMAN, Serine threonine kinase 33, Serine/threonine kinase 33, Serine/threonine-protein kinase 33, TYDP, TYDP1_HUMAN, Tyr-DNA phosphodiesterase 1, Tyrosyl-DNA phosphodiesterase 1
Recommended products
MW 764.7 Da, Purity >97%. Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
Key facts
- CAS number
- 126150-97-8
- Purity
- > 97%
- Form
- Solid
- Molecular weight
- 764.7 Da
- Molecular formula
- C34H40N2O18
- PubChem identifier
- 2293
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 100 mM.
- Biochemical name
- Bapta-AM
- Biological description
Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
- Canonical SMILES
- CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
- InChI
- InChI=1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3
- InChIKey
- YJIYWYAMZFVECX-UHFFFAOYSA-N
- IUPAC name
- acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]anilino]acetate
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TDP1 also known as Tyrosyl-DNA Phosphodiesterase 1 or TDP1p is an enzyme that plays an important role in DNA repair. It has a molecular mass of approximately 67 kDa and is expressed ubiquitously across various human tissues suggesting its fundamental role in maintaining genomic stability. Mechanically TDP1 acts by cleaving the phosphodiester bond between a tyrosine residue and a DNA 3' terminus which is important in resolving protein-DNA complexes that result from DNA damage.
- Biological function summary
This enzyme is indispensable in the repair of topoisomerase I-mediated DNA damage participating as an important player in the DNA repair machinery. TDP1 works within the context of a complex involving other repair proteins ensuring the removal of DNA-protein cross-links that could otherwise lead to cell death if left unrepaired. This activity highlights its role in genomic stability and cell viability.
- Pathways
TDP1 finds its place within the DNA single-strand break repair and the DNA damage response pathways. Its function is closely associated with other proteins such as XRCC1 which enhances the stability and efficiency of the DNA repair process and PARP1 which directs the cell's response to DNA damage. These interactions highlight TDP1's role in safeguarding genetic information by enabling precise and effective repair mechanisms.
- Associated diseases and disorders
TDP1 is notably implicated in neurodegenerative conditions such as Spinocerebellar Ataxia with Axonal Neuropathy (SCAN1) demonstrating how critical its proper function is for neuronal survival. Mutations or dysregulation in TDP1 have also been linked to heightened sensitivity to chemotherapeutic agents indicating potential challenges in cancer treatment. These disease linkages put TDP1 in association with proteins like ATM and BRCA1 which are involved in cellular responses to DNA damage and maintaining genomic integrity.
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2 product images
Chemical Structure - BAPTA-AM, Ca2+ chelator (ab120503)
2D chemical structure image of ab120503, BAPTA-AM, Ca2+ chelator
Immunocytochemistry/ Immunofluorescence - BAPTA-AM, Ca2+ chelator (ab120503)
Anti-PAI1 antibody ab66705 staining PAI1 in HepG2 cells treated with BAPTA-AM (ab120503), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of BAPTA-AM, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120503 (BAPTA-AM) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-PAI1 antibody ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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