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AB143463

BCECF-AM, intracellular ratiometric pH indicator

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(3 Publications)

MW 556.48 g/mol, Purity >98%. Acetoxymethyl ester of BCECF. Intracellular ratiometric pH indicator. Converts to fluorescent BCECF (ab120632) via the action of intracellular esterases. Ratio of fluorescence intensities can be converted to pH between 4 and 9. Excitation: 500 nm (high pH), 450 nm (low pH). Emission: 531 nm.
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Chemical Structure - BCECF-AM, intracellular ratiometric pH indicator (AB143463)
  • Chemical Structure

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Chemical Structure - BCECF-AM, intracellular ratiometric pH indicator (AB143463)

2D chemical structure image of ab143463, BCECF-AM, intracellular ratiometric pH indicator

Key facts

CAS number

117464-70-7

Purity

>98%

Form

Solid

form

Molecular weight

556.48 g/mol

Molecular formula

C<sub>3</sub><sub>0</sub>H<sub>2</sub><sub>0</sub>O<sub>1</sub><sub>1</sub>

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Acetoxymethyl ester of BCECF. Intracellular ratiometric pH indicator. Converts to fluorescent BCECF (ab120632) via the action of intracellular esterases. Ratio of fluorescence intensities can be converted to pH between 4 and 9. Excitation: 500 nm (high pH), 450 nm (low pH). Emission: 531 nm.

IUPAC Name

(2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-carboxyfluorescein) acetoxymethyl ester

Excitation/Emission

Ex: 450nm, Em: 531nm

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions
Handling instructions

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

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Refer to SDS for further information.

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Product protocols

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:284 PubMed35317437

2022

pH regulates the lumen diameter of tissue-engineered capillaries.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Wang,Jing Li,Yongqian Bian,Congying Zhao,Jinqing Li,Xueyong Li

International journal of molecular sciences 22: PubMed33804289

2021

Screening of 5- and 6-Substituted Amiloride Libraries Identifies Dual-uPA/NHE1 Active and Single Target-Selective Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Buckley,Ashna Kumar,Ashraf Aboelela,Richard S Bujaroski,Xiuju Li,Hiwa Majed,Larry Fliegel,Marie Ranson,Michael J Kelso

Journal of cellular physiology 236:1515-1528 PubMed32841374

2020

Characterization of intracellular buffering power in human induced pluripotent stem cells and the loss of pluripotency is delayed by acidic stimulation and increase of NHE1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Shiao-Pieng Lee,Shih-Chi Chao,Mei-Fang Chou,Shu-Fu Huang,Niann-Tzyy Dai,Gwo-Jang Wu,Chien-Sung Tsai,Shih-Hurng Loh,Yi-Ting Tsai
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