MW 356.18 g/mol, Purity >98%.
Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.
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- Chemistry & biology 13:957-632006The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAi-Sun Tseng,Felix B Engel,Mark T KeatingPubMed 16984885
- Journal of medicinal chemistry 47:935-462004Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanagiotis Polychronopoulos,Prokopios Magiatis,Alexios-Leandros Skaltsounis,Vassilios Myrianthopoulos,Emmanuel Mikros,Aldo Tarricone,Andrea Musacchio,S Mark Roe,Laurence Pearl,Maryse Leost,Paul Greengard,Laurent MeijerPubMed 14761195
- Chemistry & biology 10:1255-662004GSK-3-selective inhibitors derived from Tyrian purple indirubins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurent Meijer,Alexios-Leandros Skaltsounis,Prokopios Magiatis,Panagiotis Polychronopoulos,Marie Knockaert,Maryse Leost,Xiaozhou P Ryan,Claudia Alin Vonica,Ali Brivanlou,Rana Dajani,Claudia Crovace,Cataldo Tarricone,Andrea Musacchio,S Mark Roe,Laurence Pearl,Paul GreengardPubMed 14700633
Key facts
- CAS number
667463-62-9
- Purity
> 98%
- Form
Solid
- Molecular weight
356.18 g/mol
- Nature
Synthetic
Recommended products
MW 356.18 g/mol, Purity >98%.
Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.
.
Key facts
- CAS number
667463-62-9
- Purity
> 98%
- Form
Solid
- Molecular weight
356.18 g/mol
- Nature
Synthetic
- Solubility
Soluble in DMSO to 25 mM.
- Biological description
Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.
- Isomeric SMILES
O/N=C1/C(=C2C(=O)NC3CC(BR)CCC/23)NC2CCCCC12
- IUPAC name
(2'Z,3'E)-6-Bromoindirubin-3'-oxime
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
It is important to note that this product is reported to be light sensitive, Store in the dark, Store under desiccating conditions
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2 product images
Chemical Structure - BIO, GSK3 inhibitor; cell-permeable (ab120891)
2D chemical structure image of ab120891, BIO, GSK3 inhibitor; cell-permeable
Immunocytochemistry/ Immunofluorescence - BIO, GSK3 inhibitor; cell-permeable (ab120891)
Anti-beta Catenin antibody [E247] - ChIP Grade ab32572 staining ßcatenin in SW480 cells treated with BIO (ab120891), by ICC/IF. Increase of ßcatenin expression correlates with increased concentration of BIO, as described in literature.
The cells were incubated at 37°C for 48h in media containing different concentrations of ab120891 (BIO) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-beta Catenin antibody [E247] - ChIP Grade ab32572 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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