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AB120891

BIO, GSK3 inhibitor; cell-permeable

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(1 Publication)

MW 356.18 g/mol, Purity >98%. Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.
2 Images
Immunocytochemistry/ Immunofluorescence - BIO, GSK3 inhibitor; cell-permeable (AB120891)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - BIO, GSK3 inhibitor; cell-permeable (AB120891)

ab32572 staining ßcatenin in SW480 cells treated with BIO (ab120891), by ICC/IF. Increase of ßcatenin expression correlates with increased concentration of BIO, as described in literature.
The cells were incubated at 37°C for 48h in media containing different concentrations of ab120891 (BIO) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - BIO, GSK3 inhibitor; cell-permeable (AB120891)
  • Chemical Structure

Lab

Chemical Structure - BIO, GSK3 inhibitor; cell-permeable (AB120891)

2D chemical structure image of ab120891, BIO, GSK3 inhibitor; cell-permeable

Key facts

CAS number

667463-62-9

Purity

>98%

Form

Solid

form

Molecular weight

356.18 g/mol

Nature

Synthetic

Solubility

Soluble in DMSO to 25 mM

Biological description

Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.

Isomeric smiles

O/N=C1/C(=C2C(=O)NC3CC(BR)CCC/23)NC2CCCCC12

IUPAC Name

(2'Z,3'E)-6-Bromoindirubin-3'-oxime

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Complementary Products

Biochemicals

AB120962

SB 415286, GSK3 inhibitor

Chemical Structure - SB 415286, GSK3 inhibitor (AB120962)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Biochemicals

AB120488

Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor

Functional Studies - Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor (AB120488)

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Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

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  • 75
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
It is important to note that this product is reported to be light sensitive|Store in the dark|Store under desiccating conditions
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Product protocols

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 61:5129-5141 PubMed38167971

2024

Palmitic Acid Induces Posttranslational Modifications of Tau Protein in Alzheimer's Disease-Related Epitopes and Increases Intraneuronal Tau Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Melissa García-Cruz,Clorinda Arias
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