Biotin-dextran

Key facts

Purity

>98%

Form

Solid

form

Molecular weight

73400 g/mol

Nature

Synthetic

Solubility

Soluble in DMSO

Soluble in water

Biological description

Membrane impermeant dextran conjugate containing covalently attached biotin molecules (Vitamin H) making it useful in streptavidin/avidin assay systems.

The attached biotins are commonly used haptens that can be detected by using its strong affinity for the proteins avidin (from egg white) or streptavidin. This conjugate contains approximately 12 biotin groups/dextran (based on amine assay; with approx. 1 amine/40 glucose residues). The excess amino groups are not capped, and they can be further labeled with a fluorophore, or can be fixed in place with formaldehyde, lysine or glutaraldehyde, allowing subsequent tissue processing, such as sectioning.