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AB145596

Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain

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(2 Publications)

MW 533.9 Da, Purity >98%. Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33342 (ab145596) analog.
1 Images
Chemical Structure - Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain (AB145596)
  • Chemical Structure

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Chemical Structure - Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain (AB145596)

2D chemical structure image of ab145596, Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain

Key facts

CAS number

23491-45-4

Purity

>98%

Form

Solid

form

Molecular weight

533.9 Da

Molecular formula

C<sub>2</sub><sub>5</sub>H<sub>2</sub><sub>7</sub>Cl<sub>3</sub>N<sub>6</sub>O

PubChem

5360743

Nature

Synthetic

Solubility

Soluble in water to 10 mM

Biochemical name

CID 5360743

Biological description

Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33342 (ab145596) analog.

Canonical smiles

CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl

Isomeric smiles

CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl

InChi

InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H

InChiKey

OKQAASMPIOSOEX-UHFFFAOYSA-N

IUPAC Name

4-[5-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one;trihydrochloride

Excitation/Emission

Ex: 350nm, Em: 461nm

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Biochemicals

AB145597

Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain

Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (AB145597)

  • 0
  • 0
Assay Kits

AB228550

Hoechst 33258 Staining Dye Solution

Spectrum - Hoechst 33258 Staining Dye Solution (AB228550)

  • 0
  • 0
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 2178 bisBenzimide H 33258. 2178-100 is the same size as the 100 mg size of ab145596.
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2209042119 PubMed36136978

2022

Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Fengwen Zhang,Trinity M Zang,Eva M Stevenson,Xiao Lei,Dennis C Copertino,Talia M Mota,Julie Boucau,Wilfredo F Garcia-Beltran,R Brad Jones,Paul D Bieniasz

Redox biology 4:48-59 PubMed25498967

2014

The role of the catecholic and the electrophilic moieties of caffeic acid in Nrf2/Keap1 pathway activation in ovarian carcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

R Sirota,D Gibson,R Kohen
View all publications
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