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AB145597

Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain

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(16 Publications)

MW 561.9 Da, Purity >98%. Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.
1 Images
Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (AB145597)
  • Chemical Structure

Lab

Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (AB145597)

2D chemical structure image of ab145597, Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain

Key facts

CAS number

875756-97-1

Purity

>98%

Form

Solid

form

Molecular weight

561.9 Da

Molecular formula

C<sub>2</sub><sub>7</sub>H<sub>3</sub><sub>1</sub>Cl<sub>3</sub>N<sub>6</sub>O

PubChem

16760503

Nature

Synthetic

Solubility

Soluble in water to 75 mM

Biochemical name

Bisbenzimide ethoxide trihydrochloride

Biological description

Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.

Canonical smiles

CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl

InChi

InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H

InChiKey

JABNPSKWVNCGMX-UHFFFAOYSA-N

IUPAC Name

2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole;trihydrochloride

Excitation/Emission

Ex: 360nm, Em: 600nm

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Complementary Products

Reagents, Controls & Accessories

AB176753

Phalloidin-iFluor 488 Reagent

Immunocytochemistry/ Immunofluorescence - Phalloidin-iFluor 488 Reagent (AB176753)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Assay Kits

AB228551

Hoechst 33342 Staining Dye Solution

Spectrum - Hoechst 33342 Staining Dye Solution (AB228551)

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 2179 bisBenzimide H 33342. 2179-100 is the same size as the 100 mg size of ab145597.
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23906 PubMed40615435

2025

Real-time assessment of circulating tumor cells refines the indication for HER2-targeted therapy in metastatic gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuaki Kimura,Koichi Suzuki,Sawako Tamaki,Iku Abe,Yuhei Endo,Kosuke Ichida,Yuta Muto,Fumiaki Watanabe,Masaaki Saito,Kazuo Takeda,Toshiki Rikiyama

Nature 634:986-994 PubMed39358506

2024

RNA mC oxidation by TET2 regulates chromatin state and leukaemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Zou,Xiaoyang Dou,Ying Li,Zijie Zhang,Juan Wang,Boyang Gao,Yu Xiao,Yiding Wang,Lijie Zhao,Chenxi Sun,Qinzhe Liu,Xianbin Yu,Hao Wang,Juyeong Hong,Qing Dai,Feng-Chun Yang,Mingjiang Xu,Chuan He

Molecular cell 83:4304-4317.e8 PubMed37949069

2023

FMRP phosphorylation modulates neuronal translation through YTHDF1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Zou,Jiangbo Wei,Yantao Chen,Yunhee Kang,Hailing Shi,Fan Yang,Zhuoyue Shi,Shijie Chen,Ying Zhou,Caraline Sepich-Poore,Xiaoxi Zhuang,Xiaoming Zhou,Hualiang Jiang,Zhexing Wen,Peng Jin,Cheng Luo,Chuan He

Nature 607:756-761 PubMed35859172

2022

Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex I.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Rodríguez-Nuevo,Ariadna Torres-Sanchez,Juan M Duran,Cristian De Guirior,Maria Angeles Martínez-Zamora,Elvan Böke

EMBO reports 23:e54049 PubMed35253958

2022

Intra-epithelial non-canonical Activin A signaling safeguards prostate progenitor quiescence.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Cambuli,Veronica Foletto,Alessandro Alaimo,Dario De Felice,Francesco Gandolfi,Maria Dilia Palumbieri,Michela Zaffagni,Sacha Genovesi,Marco Lorenzoni,Martina Celotti,Emiliana Bertossio,Giosuè Mazzero,Arianna Bertossi,Alessandra Bisio,Francesco Berardinelli,Antonio Antoccia,Marco Gaspari,Mattia Barbareschi,Michelangelo Fiorentino,Michael M Shen,Massimo Loda,Alessandro Romanel,Andrea Lunardi

Cancer letters 534:215612 PubMed35259458

2022

ETS-related gene (ERG) undermines genome stability in mouse prostate progenitors via Gsk3β dependent Nkx3.1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Lorenzoni,Dario De Felice,Giulia Beccaceci,Giorgia Di Donato,Veronica Foletto,Sacha Genovesi,Arianna Bertossi,Francesco Cambuli,Francesca Lorenzin,Aurora Savino,Lidia Avalle,Alessia Cimadamore,Rodolfo Montironi,Veronica Weber,Francesco Giuseppe Carbone,Mattia Barbareschi,Francesca Demichelis,Alessandro Romanel,Valeria Poli,Giannino Del Sal,Marianna Kruithof-de Julio,Marco Gaspari,Alessandro Alaimo,Andrea Lunardi

Anticancer research 42:407-417 PubMed34969751

2022

High-purity Isolation for Genotyping Rare Cancer Cells from Blood Using a Microfluidic Chip Cell Sorter.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Ikeda,Yasuhiro Koh,Jun Oyanagi,Shunsuke Teraoka,Masayuki Ishige,Yuu Fujimura,Kazuo Takeda,Nahomi Tokudome,Yuichi Ozawa,Hiroki Ueda,Nobuyuki Yamamoto

Veterinary microbiology 255:109017 PubMed33639390

2021

Bovine respiratory coronavirus enhances bacterial adherence by upregulating expression of cellular receptors on bovine respiratory epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Watcharapong Fahkrajang,Putu Eka Sudaryatma,Hirohisa Mekata,Saori Hamabe,Akatsuki Saito,Tamaki Okabayashi

Journal of neurointerventional surgery : PubMed33106319

2020

Endothelialized silicone aneurysm models for in vitro evaluation of flow diverters.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa McCulloch,Ashley Turcott,Gabriella Graham,Sergey Frenklakh,Kristen O'Halloran Cardinal

Veterinary microbiology 246:108748 PubMed32605748

2020

Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Putu Eka Sudaryatma,Akatsuki Saito,Hirohisa Mekata,Meiko Kubo,Watcharapong Fahkrajang,Tamaki Okabayashi
View all publications
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