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MW 561.9 Da, Purity >98%. Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.

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Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (AB145597), expandable thumbnail

Publications

Key facts

CAS number
875756-97-1
Purity
> 98%
Form
Solid
Molecular weight
561.9 Da
Molecular formula
C27H31Cl3N6O
PubChem identifier
16760503
Nature
Synthetic
Excitation/Emission
Ex: 360nm, Em: 600nm

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MW 561.9 Da, Purity >98%. Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.

Key facts

Purity
> 98%
PubChem identifier
16760503
Solubility

Soluble in water to 75 mM.

Biochemical name
Bisbenzimide ethoxide trihydrochloride
Biological description

Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33258 (ab145596) analog.

Canonical SMILES
CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl
InChI
InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
InChIKey
JABNPSKWVNCGMX-UHFFFAOYSA-N
IUPAC name
2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole;trihydrochloride
Excitation/Emission
Ex: 360nm, Em: 600nm

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions, The product can be stored for up to 12 months

Notes

This product is manufactured by BioVision, an Abcam company and was previously called 2179 bisBenzimide H 33342. 2179-100 is the same size as the 100 mg size of ab145597.

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1 product image

  • Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (ab145597), expandable thumbnail

    Chemical Structure - Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (ab145597)

    2D chemical structure image of ab145597, Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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